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  • 1
    Electronic Resource
    Electronic Resource
    ESR Study of Nitroxide Radicals Generated from Triaz-2-en-1-ols (1996)
    New York, NY : Wiley-Blackwell
    Helvetica Chimica Acta 79 (1996), S. 663-669 
    Details
    ISSN: 0018-019X
    Keywords: Chemistry ; Organic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The triazenols 4-R1—C6H4—N=N—N(OH)—R2 (1), oxidized with t-BuO⋅2 radicals, produced nitroxide radicals R1—C6H4—N(O⋅)—N=N(R2) +O- (5). The suggested radical structure was confirmed by 15N-labeling. The reaction of triazenols 1 with PbO2 proceeded under N2 elimination, in which case nitroxides R1—C6H4—N(R2)—O⋅(2) were observed as the final radical products. The intermediate R1—C6H4⋅ radicals were identified by spin-trapping.
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/hlca.19960790310
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  • 2
    Electronic Resource
    Electronic Resource
    Autocrine and paracrine growth stimulation of cells derived from human skin (1983)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 117 (1983), S. 235-240 
    Details
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: A sensitive serum-free culture system was used to demonstrate that cells derived from normal human skin release soluble mediators which can modulate keratinocyte, melanocyte, and fibroblast growth in vitro. In M199 supplemented with epidermal growth factor, hydrocortisone, insulin, transferrin, triidothyronine, bovine serum albumin, and an extract of bovine hypothalamus, keratinocytes underwent approximately three to five cumulative population doublings (CPD) over a 7-day period. Addition of 20% keratinocyte-conditioned medium more than doubled keratinocyte growth and increased fibroblast growth 25-40% above controls. Dermal fibroblasts maintained in the same serum-free hormone-supplemented medium (SFHSM) alone underwent approximately 4.5-5.5 CPD over a 7-day period; 20% fibroblast-conditioned medium increased fibroblast growth 50-80% and increased keratinocyte growth 30-50%. Melanocytes maintained in the same SFHSM underwent approximately 1 CPD over a 14-day period and 2 CPD if the medium was supplemented with 2% fetal bovine serum (FBS). Addition of 20% melanocyte-conditioned medium more than doubled melanocyte growth in either SFHSM or in medium containing 2% FBS, but decreased both keratinocyte and fibroblast growth up to 30-60%. None of the conditioned media altered cellular morphology. These data provide the first demonstration of mutual growth modulation by cell types normally contiguous in vivo and expand existing evidence for autocrine and paracrine growth regulation by normal human cells.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcp.1041170215
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  • 3
    Electronic Resource
    Electronic Resource
    Growth characteristics of human epidermal melanocytes in pure culture with special reference to genetic differences (1988)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 135 (1988), S. 262-268 
    Details
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Human melanocyte cultures were established using disaggregated epidermal cell suspensions derived from foreskins and plated onto culture dishes in medium containing 2% fetal bovine serum, growth factors, hormones, and melanocyte growth factor (MGF) extracted from bovine hypothalamus (Wilkins et al., J. Cell. Physiol., 122: 350, 1985). After 2 days in culture the cells were transferred to serum-free medium to eliminate keratinocyte and fibroblast growth. Melanocytes grew preferentially and pure melanocyte populations could be harvested after 12-16 days in vitro. Melanocytes were later subcultured in the presence of 1% FBS. Pure melanocyte cultures were characterized by light and electron microscopic criteria, as well as by cytochemical demonstration of the melanocytes specific enzyme, tyrosinase. At the ultrastructural level, cultured melanocytes derived from black (negroid) neonatal skin (B-M) had numerous mature rodshaped stage IV melanosomes, while white (caucasoid) skin-derived melanocytes (W-M) in culture contained no mature melanosomes. Growth rate, cell yield, and in vitro lifespan for B-M were more than twice that for W-M in pure melanocyte cultures in the presence of MGF. Our results suggest that MGF-dependent growth of B-M differs from that of W-M.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcp.1041350213
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  • 4
    Electronic Resource
    Electronic Resource
    Darstellung und einige Eigenschaften von Kupfer(I)-Kupfer(II)-Selenocyanaten (1975)
    Weinheim : Wiley-Blackwell
    Zeitschrift für anorganische Chemie 417 (1975), S. 154-160 
    Details
    ISSN: 0044-2313
    Keywords: Chemistry ; Inorganic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Description / Table of Contents: Preparation and Some Characteristics of Copper(I)-Copper(II) SelenocyanatesFrom the system Cu2+-H2O-en-SeCN-in dependence on the conditions two new complexes have been prepared: Cu3en2(SeCN)(CN)3· H2O(I) and Cu3en2(SeCN)2(CN)2· H2O(II). They were studied by the methods of infra-red and EPR spectra as well as by those of X-ray diffraction. It has been found that Cu2+ is coordinated in these complexes with two molecules of ethylene diamine and one molecule water. The anion ligands have a bridging function and coordinate the copper(I).The EPR spectra show that the two complexes exhibit around the atom Cu2+ have different ligand field symmetry. For complex (I) it is a typically axial spectrum with two values of the gfactor g⊥—2.18 and g⊥—2.05 while complex (II) has an asymmetrical isotropic spectrum with one g-factor value only g⊥—2.07.
    Notes: Aus dem System Cu2+-H2O-en-SeCN-wurden in Abhängigkeit von den Bedingungen zwei neue Komplexe hergestellt: Cu3en2(SeCN)(CN)3. H2O(I) und Cu3en2 (SeCN)2(CN)2. H2O(II). Ihre Untersuchung erfolgte mittels der Infrarot- und EPR-Spektroskopie sowie der Röntgendifraktion. Cu2+ liegt in diesen Komplexen durch zwei Äthylendiamin- und eine Wassermolekel koordiniert vor. Die Anionliganden haben eine Brückenfunktion und binden das einwertige Kupfer. Die EPR-Spektren zeigen, daß die Ligandenfeldsymmetrie um das Cu2+- Atom bei den beiden Komplexen nicht gleich ist. Komplex (I) hat ein typisch axiales Spektrum mit zwei g-Faktorwerten g⊥—2,18 und g⊥—2,05, während Komplex (II) ein asymmetrisches, isotropes Spektrum aufweist, mit einem Wert von g⊥—2,07.
    Additional Material: 2 Tab.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/zaac.19754170212
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  • 5
    Electronic Resource
    Electronic Resource
    Spektrale Untersuchungen von Cyanidkomplexen mit ein- und zwiwertigem Kupfer (1975)
    Weinheim : Wiley-Blackwell
    Zeitschrift für anorganische Chemie 417 (1975), S. 229-234 
    Details
    ISSN: 0044-2313
    Keywords: Chemistry ; Inorganic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Description / Table of Contents: Spectral Properties of Cyanocopper(I)-Copper(II) CompoundsThe spectral properties of the compounds: Cu 3 (CN) 4 (NH 3 ) 3, Cu 5 (CN) 6 (NH 3 ) 4, Cu 3 (CN) 4 en 2 H 2 O, Cu 4 (CN) 6 en 2 H 2 O, Cu 5 (CN) 6 en 2 and Cu 3 (CN) 4 (H 2 O) 5 were studied. On the basis of the results of spectral informations in relation with the results of X-ray analysis of the first three compounds, bond posibilities of the CN group and the way of coordination at copper(I) and copper(II) in the investigated compounds of unknown structure are discussed.
    Notes: Die spektralen Eigenschaften der Komplexe Cu 3 (CN) 4 (NH 3) 3, Cu 5 (CN) 6 (NH 3) 4, Cu 3 (CN) 4 en 2 H 2 O, Cu 4 (CN) 6 en 2 H 2 O, Cu 5 (CN) 6 en 2 und Cu 3 (CN) 4 (H 2 O) 5 werden untersucht. Durch Vergleich der Spektren mit den Ergebnissen einer Röntgen-Strukturanalyse der ersten drei Komplexe wird die Bindungsmöglichkeit der Cyanidgruppe und die Art der Koordination des ein- und zweiwertigen Kupfers in den untersuchten Komplexen unbekannter Struktur diskutiert.
    Additional Material: 3 Tab.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/zaac.19754170307
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