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  • 1
    Electronic Resource
    Electronic Resource
    New York, NY : Wiley-Blackwell
    Gamete Research 18 (1987), S. 97-107 
    ISSN: 0148-7280
    Keywords: protein synthesis ; oocyte ; human ; electrophoresis ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: As a first step toward understanding control of gene expression in early human development, an analysis of protein synthesis and amino-acid transport in unfertilized mature oocytes was initiated. Qualitative patterns of protein synthesis were examined in individual oocytes cultured in medium containing radiolabeled methionine. No differences in synthetic pattern of proteins, resolved by one-dimensional electrophoresis and fluorography, were observed in oocytes analyzed from times varying from 12 to 52 hr following collection by laparoscopy. Contamination by follicular or corona radiata cells was readily distinguished on the basis of increased relative synthesis of a polypeptide with Mr = 44,000, a dominant product of synthesis in follicular cells. Based on the specific activity of the methionine precursor, the absolute rate of synthesis was measured to be about 50 pg/oocytc/hr, a value higher than in mouse unfertilized eggs. No difference in protein synthetic rate was observed in oocytes analyzed at 12 hr postcollection versus later times up to 50 hr postcollection. Competition of methionine uptake by leucine, efflux of radiolabeled methionine from preloaded oocytes into medium containing methionine and uptake of methionine in medium with low sodium ion concentration was observed. These findings are consistent with the presence of an L (leucine-preferring) system for neutral amino acid transport, similar to that in mouse and rabbit eggs. Total protein was measured to be about 150 ng/oocyte, a value five times that of the mouse. These studies provide basic data for further analysis of oocytes and perhaps preimplantation stage embryos in the future.
    Additional Material: 1 Ill.
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  • 2
    Electronic Resource
    Electronic Resource
    New York, NY : Wiley-Blackwell
    Gamete Research 15 (1986), S. 1-12 
    ISSN: 0148-7280
    Keywords: methionine uptake ; methionine efflux ; kinetic parameters ; trans-stimulation ; trans-inhibition ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: The mouse egg is an ideal model for the kinetic study of neutral amino acid exchange transport in development in that it possesses strong exchange-mediated uptake/efflux with little other efflux. Exchange transport was examined by preloading eggs in unlabeled or labeled amino acid and then measuring subsequent amino acid fluxes. The existence of two L-like (leucine-preferring) systems, a low-affinity exchange system, and a high-affinity weakly exchanging system is suggested by the differences between preloaded and control eggs in uptake specificity and in the kinetic parameters of methionine uptake (respectively, Km = 220 and 69 μM, Vmax = 98 and 32 fmol/min/egg). The Vmax of exchange-mediated efflux of methionine is similar to that for uptake into preloaded eggs, but the Km is about an order of magnitude higher. Preloading in alanine and serine (preferred substrates, along with cysteine, of system ASC) decreased the subsequent uptake of radiolabeled methionine, suggesting some ASC activity in the unfertilized mouse egg. The plateau in accumulation of leucine and phenylalanine, characteristic of simple exchange (mediated by system L), is not shared by methionine, suggesting the existence of another system (possibly an A, or alanine-preferring, system) for the transport of methionine. Amino acid transport in the mouse egg is more complex than previously thought, the egg possessing four interacting systems for the transport of the neutral amino acids.
    Additional Material: 4 Ill.
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  • 3
    Electronic Resource
    Electronic Resource
    Weinheim : Wiley-Blackwell
    Liebigs Annalen 196 (1879), S. 32-57 
    ISSN: 0075-4617
    Keywords: Chemistry ; Organic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Weinheim : Wiley-Blackwell
    Liebigs Annalen 203 (1880), S. 118-137 
    ISSN: 0075-4617
    Keywords: Chemistry ; Organic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Weinheim : Wiley-Blackwell
    Liebigs Annalen 207 (1881), S. 320-347 
    ISSN: 0075-4617
    Keywords: Chemistry ; Organic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Weinheim : Wiley-Blackwell
    Liebigs Annalen 207 (1881), S. 348-360 
    ISSN: 0075-4617
    Keywords: Chemistry ; Organic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    Weinheim : Wiley-Blackwell
    Liebigs Annalen 207 (1881), S. 361-368 
    ISSN: 0075-4617
    Keywords: Chemistry ; Organic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    Weinheim : Wiley-Blackwell
    Chemie Ingenieur Technik - CIT 42 (1970), S. 1441-1444 
    ISSN: 0009-286X
    Keywords: Chemistry ; Industrial Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Additional Material: 8 Ill.
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  • 9
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Applied Polymer Science 29 (1984), S. 2493-2500 
    ISSN: 0021-8995
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: In this work we have studied the stabilizing effect of several commercially available antioxidants on the free-radical photooxidation of butadiene rubber. Two kinds of stabilizers were investigated; hindered phenols: 2,4-di-t-butyl-p-hydroxytoluene, octadecyl-3-(3,5-di-t-butyl-4-hydroxyphenyl)-propionate and 2,4-bis(octylthio)-6-(4-hydroxy-3,5-di-t-butylanilino)-1,3,5-triazine; and amine stabilizers: N-isopropyl-N′-phenyl-p-phenylenediamine and N-(1,3-dimethylbutyl)-N′-phenyl-p-phenylenediamine. Their stabilizing effect is mostly produced by scavenging the peroxy radicals formed during the initial steps of the photodegradation process. We also tested tri(nonylphenyl)phosphite associated with the phenoltriazine stabilizer. Of the additives studied in this work, octadecyl-3-(3,5-di-t-butyl-4-hydroxyphenyl)propionate was the most effective photostabilizer for butadiene rubber.
    Additional Material: 4 Ill.
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  • 10
    Electronic Resource
    Electronic Resource
    Weinheim : Wiley-Blackwell
    Liebigs Annalen 216 (1883), S. 232-240 
    ISSN: 0075-4617
    Keywords: Chemistry ; Organic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
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