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  • 1
    Electronic Resource
    Electronic Resource
    New York, N.Y. : Wiley-Blackwell
    Journal of Supramolecular Structure 11 (1979), S. 327-338 
    ISSN: 0091-7419
    Keywords: AcChR-enriched membranes ; pyrenesulfonyl azide ; fluorescent probes ; photolabeling ; Life Sciences ; Molecular Cell Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Acetylcholine receptor (AcChR) enriched membrane fragments from Torpedo californica electroplax were labeled by in situ photogenerated nitrenes from a hydrophobic fluorescent probe, pyrene-1-sulfonyl azide. Preferential photolabeling of membrane proteins, mainly AcChR, has been achieved and there is a pronounced exposure of the 48,000 and 55,000 molecular weight subunits of AcChR to the lipid environment of the membrane core.Covalent attachment of the photogenerated fluorescence probe does not perturb the α-neurotoxins' binding properties of membrane-bound AcChR or the desensitization kinetics induced by prolonged exposures to cholinergic agonists. Non-covalent photoproducts can be conveniently removed from labeled membrane preparations by exchange into lipid vesicles prepared from electroplax membrane lipids. Fluorescence features of model pyrene sulfonyl amide derivatives, such as fine vibrational structure of emission spectra or fluorescence lifetimes, are highly sensitive to the solvent milieu. The covalently bound probe shows similar fluorescence properties in situ. PySA photoproducts have great potential to spectroscopically monitor neurotransmitter induced events on selected AcChR subunits exposed to the hydrophobic environment of membranes.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Weinheim : Wiley-Blackwell
    Propellants, Explosives, Pyrotechnics 12 (1987), S. 164-166 
    ISSN: 0721-3115
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Afin d'améliorer les délais de réponse et la productivité, une méthode d'analyse autre que la méthode Dewarda était recherchée pour doser I'azote nitrique des nitrocelluloses, avec des succès divers mais sans obtenir la précision demandée. Les auteurs ont étudié I'influence des paramètres régissant le dosage par potentiometrie de l'azote nitrique par le sulfate ferreux, tels que la température, la stabilité des réactifs, les concentrations, I'agitation. Cette étude a permis de concevoir un prototype de dosage entitrèment automatisé, permettant d'obtenir un temps de réponse de l'ordre de 20 minutes et une précision comparable à celle de la méthode Dewarda. Elle s'applique à la nitrocellulose. aux esters nitriques et à la nitroguanidine.
    Additional Material: 3 Tab.
    Type of Medium: Electronic Resource
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