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  • 1
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Applied Polymer Science 10 (1966), S. 1673-1685 
    ISSN: 0021-8995
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Thermal diffusivity, thermal conductivity, and specific heat per unit volume were measured for the following polymers: poly(ethylene terephthalate), polytetrafluoroethylene, polycarbonate, polypropylene, and three poly(vinyl chloride) samples plasticized to different levels. First- and second-order transitions can be identified and located by the following features in the thermal property-temperature curves: discontinuities, sharp inflections, broad inflections, sharp maxima, broad maxima, and change in linear slope. The results for poly(vinyl chloride) indicate the possibility of the use of plasticizer to control the thermal insulation properties of polymers, both for steady-state and unsteady-state conduction.
    Additional Material: 16 Ill.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Weinheim : Wiley-Blackwell
    Electrophoresis 10 (1989), S. 283-290 
    ISSN: 0173-0835
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: The migration of a series of supercoiled plasmids ranging in size from 4 to 91 kilobases (kb) has been analyzed by orthogonal-field-alternation gel electrophoresis (OFAGE). These circular DNAs enter OFAGE gels and are resolved over the same region of the gel as linear DNAs from 260 to 2200 kb. Furthermore, a distinct triphasic migration pattern was observed for the supercoiled DNAs. The migration of plasmids between 6 and 20, and 60 and 91 kb is inversely proportional to size, whereas the mobilities of plasmids between 20 and 60 kb increase with size. Unlike linear DNA molecules, the relative mobilities of these plasmids are constant over a broad range of pulse times, from 10 to 120 s. Electrophoresis of supercoiled, relaxed, and nicked open circular forms as well as topoisomers of small plasmids shows that the extent of supercoiling has a dramatic effect on plasmid migration on OFAGE. Several practical applications for exploiting the different migration properties of circular and linear DNA molecules on OFAGE are presented.
    Additional Material: 7 Ill.
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  • 3
    Electronic Resource
    Electronic Resource
    Hoboken, NJ : Wiley-Blackwell
    Journal of Biomedical Materials Research 40 (1998), S. 282-290 
    ISSN: 0021-9304
    Keywords: dental implants ; bone formation ; cell differentiation ; osteointegration ; dental material ; bone substitutes ; Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine , Technology
    Notes: A proposed in vitro system is described where chick osteoblasts are cultured on the flat surfaces of dense, nonporous HA disks to facilitate the study of bone formation at the cell-HA interface. During early bone formation cell-coated HA disks were retrieved, fixed with buffered 2% glutaraldehyde, and embedded in epon/araldite. The underlying HA disks were demineralized in diluted acid, and the intact cell-HA interfaces were re-embedded and thin sectioned for routine transmission electron microscopy. Morphologic studies indicated that osteoblasts proliferated and formed nodules of cells on the surfaces of HA disks. With increasing time in culture, they deposited orthogonally packed collagen fibrils between the cell layers that were enveloped by electron-dense mineralized globules. Eventually, small spicules of mineralized HA formed along collagen fibrils. An electron-dense layer about 50 nm thick was observed on the surface of the HA disks. Biochemical studies indicated that cell proliferation, as judged by 3H-thymidine uptake, increased rapidly during the first 3 days, reached a maximum around 6 days, and then declined by 12 days in culture. AP activity and collagen synthesis, as determined by 3H-hydroxyproline formation, increased as cellular proliferation declined. Mineralization, as judged by 45Ca uptake and spicule formation, occurred, as expected, following the increase in AP activity and deposition of densely packed collagen fibrils. Thus, all morphological and biochemical parameters studied indicate that the proposed in vitro system is reproducible and can facilitate the study of the osteointegration of HA-coated implants. © 1998 John Wiley & Sons, Inc. J Biomed Mater Res, 40, 282-290, 1998.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
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