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  • 1
    Electronic Resource
    Electronic Resource
    Physical properties of high-pressure ethylene vinylacetate copolymers and their saponified derivatives (1983)
    Stamford, Conn. [u.a.] : Wiley-Blackwell
    Polymer Engineering and Science 23 (1983), S. 306-317 
    Details
    ISSN: 0032-3888
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: The physical properties of high-pressure ethylene vinylacetate (EVA) copolymers having an average melt index of 8.5 g/10 min and a mol% of VA less than 20 are studied. A comparison is made with the properties of their saponified derivatives, the ethylene vinylalcohol (EVAl) co- and ethylene vinylalcohol vinylacetate (EVAlVA) terpolymers. A melt-index effect is noted. Density, thermal, and mechanical properties of EVA copolymers are determined by the degree of crystallinity, which depends on the mol% VA and on the degree of alkyl shortchain branches. EVAlVA terpolymer properties depend on the residual mol% VA. EVAl copolymers proved to have some properties similar to low-density polyethylene. The hydrogen-bonding effect via hydroxyl groups was negligible for this level of vinylalcohol incorporation.
    Additional Material: 27 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/pen.760230604
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  • 2
    Electronic Resource
    Electronic Resource
    N-Lithiomethyl-N,N′N″,N″-tetramethyldiethylene-triamine: The First Alkyllithium Compound which is Monomeric in Hydrocarbons (1992)
    Weinheim : Wiley-Blackwell
    Angewandte Chemie International Edition in English 31 (1992), S. 633-635 
    Details
    ISSN: 0570-0833
    Keywords: Chemistry ; General Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/anie.199206331
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  • 3
    Electronic Resource
    Electronic Resource
    Biological energy transformations in tissue homogenatesThis work was aided by a grant from the Jonathan Bowman Fund for Cancer Research. (1945)
    Philadelphia : Wiley-Blackwell
    Journal of Cellular and Comparative Physiology 26 (1945), S. 87-100 
    Details
    ISSN: 0095-9898
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcp.1030260204
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  • 4
    Electronic Resource
    Electronic Resource
    Hormonal regulation of amino acid transport and cAMP production in monolayer cultures of rat hepatocytes (1980)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 103 (1980), S. 159-168 
    Details
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The effects of insulin, glucagon or Dexamethasone (DEX) and of glucagon with insulin or DEX were examined on the uptake of 2-amino [1-14C]isobutyric acid (AIB) and N-Methyl-2-amino [1-14C]isobutyric acid (NMe AIB) in monolayer cultures of rat hepatocytes. Insulin and glucagon stimulated the uptake of both the amino acids and DEX inhibited it, showing that all three of these hormones regulate the A system (the sodium-dependent system that permits the transport of NMe AIB) for amino acid transport in these cultures. Experiments investigating the transport of aminocyclopentane-1-carboxylic acid, 1- [carboxyl-14C] in the presence of excess AIB or in the absence of sodium showed that insulin had no effect on the activity of the L system (the sodium-independent system that prefers leucine). Experiments on the uptake of AIB in the presence of excess NMe AIB showed insulin had no effect on the transport activity of the ASC system (the sodium-dependent system that does not transport NEe AIB). Insulin concentrations ranging from 0.1 nM to 100 nM did not antagonize the stimulatory effect of optimum or suboptimum concentrations of glucagon on the uptake of either AIB or NMe AIB. Similarly, glucagon did not antagonize the stimulatory effect of optimum or suboptimum concentrations of insulin on the uptake of both the amino acids. The combined effect of insulin and glucagon was additive on the rate as well as the cumulative uptake of both AIB and NMe AIB. DEX alone inhibited the transport of both AIB and NMe AIB by about 25%, while glucagon caused a 2-3-fold increase; however, the addition of glucagon to cultures containing DEX caused a 7-8-fold increase in the uptake of both AIB and NMe AIB when compared to cultures containing DEX alone. The effect of insulin on the levels of cAMP was also investigated. Insulin had no effect on the cAMP levels in cultures treated or untreated with optimum or suboptimum concentrations of glucagon.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcp.1041030120
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  • 5
    Electronic Resource
    Electronic Resource
    Effects of hormones and amino acid depletion on the kinetic parameters of amino acid uptake in monolayer cultures of rat hepatocytes (1982)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 112 (1982), S. 67-75 
    Details
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Uptake of 2-aminoisobutyric acid (AIB) at concentrations of 0.1 mM to 30 mM was examined in sodium-containing and sodium-free media in hepatocytes pretreated without hormones (control), with hormones, or with amino acid depletion. Results show that 1-minute but not 4-minute rates can be taken as initial rates for the total or sodium-dependent transport of AIB. The data for the 1-minute sodium-dependent transport of AIB were analyzed by a comptuer program and also by Eadie-Hofstee and Lineweaver-Burk plots, and a single saturable system was found. In the control cultures, the saturable system had a Km of 1-2 mM AIB and a Vmax of 1.2 nmoles AIB/mg protein/minute. There was an increase in the Vmax of two to three-fold after pretreating the cultures with insulin or amino acid depletion, three to four-fold with glucagon, and six to seven fold with glucagon + dexamethasone.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcp.1041120111
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  • 6
    Electronic Resource
    Electronic Resource
    The diverse effects of 5′-bromodeoxyuridine on enzyme activities in cultured H35 hepatoma cells (1977)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 91 (1977), S. 271-287 
    Details
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Reuber (H35) hepatoma cells were grown in medium containing 10 -5M bromodeoxyuridine (BrdU), which was incorporated into their DNA. Cell growth rate was unaffected by BrdU for the first two generations, after which it was reduced by about 50%. The effect of BrdU incorporation on the activities of several enzymes with rapid turnover rates was examined to test the hypothesis that the synthesis of such enzymes will be preferentially inhibited by BrdU. Tyrosine amino-transferase (TAT) activity decreased by 70% within two generations whereas thymidine kinase activity remained at control values. PEP carboxykinase activity was unchanged during the first generation in BrdU-containing medium but, during the second, its activity increased by at least 30%. Ornithine decarboxylase levels decreased by about 50% only after two generations in the presence of BrdU. There appeared to be no simple relationship between turnover rates and the effect of BrdU on enzyme activity. Incorporation of BrdU was found to inhibit the induction of both TAT and PEP carboxykinase by dexamethasone and to enhance the inhibition of cell growth by this steroid. These results are discussed with respect to possible mechanisms of gene expression and development in both normal and neoplastic cells.
    Additional Material: 11 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcp.1040910212
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  • 7
    Electronic Resource
    Electronic Resource
    Hormonal regulation of amino acid transport and gluconeogenesis in primary cultures of adult rat liver parenchymal cells (1976)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 89 (1976), S. 641-646 
    Details
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Amino acid transport was studied in primary cultures of parenchymal cells isolated from adult rat liver by a collagenase perfusion technique and maintained as a monolayer in a serum-free culture medium. These cells carried out gluconeogenesis from three carbon precursors (alanine, pyruvate, and lactate) in response to glucagon addition. Amino acid transport was assayed by measuring the uptake of the nonmetabolizable amino acid, α-aminoisobutyric acid (AIB). Addition of insulin or glucagon to cultured rat liver parenchymal cells resulted in an increased influx of AIB which was reflected in a higher initial rate of AIB transport. The glucocorticoid, dexamethasone, when added alone to cultures did not affect AIB transport. However, prior or simultaneous addition of dexamethasone to glucagon-treated cells caused a strong potentiation of the glucagon induction of AIB transport. Kinetic analysis of the effects of insulin and glucagon demonstrated that insulin increased the Vmax for transport without changing the Km while glucagon primarily decreased the Km for AIB transport. The effect of dexamethasone was to increase the Vmax of the low Km system.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcp.1040890421
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  • 8
    Electronic Resource
    Electronic Resource
    Summary of 1965 biology research conference (1965)
    Philadelphia : Wiley-Blackwell
    Journal of Cellular and Comparative Physiology 66 (1965), S. 175-182 
    Details
    ISSN: 0095-9898
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcp.1030660416
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