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  • 1
    Electronic Resource
    Electronic Resource
    The size of turbulent eddies close to a wall (1988)
    Hoboken, NJ : Wiley-Blackwell
    AIChE Journal 34 (1988), S. 938-945 
    Details
    ISSN: 0001-1541
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Coherent flow-oriented structures, with a dimensionless spacing of λ+ ≈ 100, observed close to a wall in turbulent flows are believed to control the production of turbulence. A 2 1/2 D computer model developed by Nikolaides is used to explain the dimensionless spacing of these structures. The calculations suggest that the net production of turbulence in the viscous wall region is sensitive to changes in λ+ in the neighborhood of λ+ ≈ 100. They indicate that the net production is negative for λ+ 〈 85 and that for λ+ = 93 the net production is large enough to supply the turbulent energy dissipated in the outer flow.
    Additional Material: 10 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/aic.690340606
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  • 2
    Electronic Resource
    Electronic Resource
    Turbulence-producing eddies in the viscous wall region (1989)
    Hoboken, NJ : Wiley-Blackwell
    AIChE Journal 35 (1989), S. 1962-1974 
    Details
    ISSN: 0001-1541
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A computer simulation of turbulent flow in a channel is used to detect flow patterns related to the production of Reynolds stress. It is found that quadrant 2 and quadrant 4 events possess a streaky structure in the viscous wall region and that these events can be best understood by examining the velocity field in the y-z plane. Large turbulence production in the viscous wall region is found to occur in updrafts and downdrafts associated with closed eddies. These eddies, on average, have a spanwise dimension of 50 wall units and a streamwise dimension of 400-450 wall units. They are often seen to originate from small attached eddies at the wall.
    Additional Material: 18 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/aic.690351207
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  • 3
    Electronic Resource
    Electronic Resource
    Cyclic voltammetry of polypyrroledodecylbenzenesulfonate layers (1995)
    Weinheim : Wiley-Blackwell
    Electroanalysis 7 (1995), S. 287-289 
    Details
    ISSN: 1040-0397
    Keywords: Polypyrrole ; Dodecylbenzenesulfonate ; Cyclic voltammetry ; Modified electrode ; Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Polypyrrole was deposited from solutions of NaClO4 and NaDBS, where DBS is dodecylbenzenesulfonate, in aqueous solution and characterized by voltammetry. It is found that the absence of the so-called ‘double layer charging current’ during cyclic voltammetry of the layers formed from solutions with surfactant is not because of the order in morphology of the layer, but irreversible incorporation of the surfactant anion.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/elan.1140070317
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  • 4
    Electronic Resource
    Electronic Resource
    Nuclear protein redistribution in heat-shocked cells (1993)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 154 (1993), S. 402-409 
    Details
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: An increase was observed in the total protein mass of nuclei isolated from Chinese hamster ovary cells heated at 45°C or 45.5°C. An increase in the fractional recovery of DNA polymerase α and β, and of DNA topoisomerase activity coincided with this increase in the protein mass of nuclei from heated cells. Nuclear protein mass which was soluble in 2.0 M NaCl decreased 0.5 fold, while DNA-associated and nuclear matrix-associated protein mass increased 2.2 and 3.4 fold, respectively. The results indicate that the increase in nuclear protein mass observed in nuclei from heated cells is due in part to an increased binding, or precipitation, of nuclear proteins onto the cell's DNA and nuclear matrix. © 1993 Wiley-Liss, Inc.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcp.1041540224
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  • 5
    Electronic Resource
    Electronic Resource
    Understanding the dynamics of cellular responsiveness to modifications of metabolic substrates in perifusion (1994)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 160 (1994), S. 10-16 
    Details
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: A novel microperifusion system with capabilities for continuous, real-time, potentiometric monitoring of extracellular hydrogen ion concentration has been used to define the response of HeLa cells to abrupt changes in extracellular energy sources or introduction of an inhibitor of glycolysis. Glycolytic inhibition, induced by removal of glucose or introduction of iodoacetate, each led to a rapid, continuous decrease in acid release. The response to iodoacetate took longer than removal of glucose, perhaps due to the time required for binding and activation. Once inhibition began, however, the rate of change was greater than following glucose removal. Conversely, recovery time following iodoacetate inhibition was much slower than with glucose removal. Unlike the response to short-term glucose depletion, a second pulse of iodoacetate resulted in a faster response followed by an even longer recovery time. The response to switching between glucose and glutamine began almost without evident delay. The response patterns revealed that HeLa cells prefer glutamine to glucose, but, in the presence of both energy sources, some glucose continues to be used. In summary, these results indicate that continuous, real-time monitoring of the kinetics of hydrogen-ion release can be used to gain new insights into the dynamics of cellular response to perturbations of extracellular energy sources. © 1994 Wiley-Liss, Inc.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcp.1041600103
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  • 6
    Electronic Resource
    Electronic Resource
    Inhibition of replicon cluster ligation into chromosomal DNA at elevated temperatures (1990)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 142 (1990), S. 365-371 
    Details
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The rate limiting enzymatic step for DNA replication in HeLa cells incubated at 43.5°C v/as the ligation of clusters of replicons into the cell's genome. At 43.5° the reciprocal slope for inhibition of DNA chain (replicon) initiation, or of the ligation of replicon clusters into the genome, was 18 or 7 min, respectively. The failure of replicon clusters to be ligated into chromosomal DNA was not a consequence of the failure of histone proteins to be deposited onto replicating DNA, or of chromatin replicated at 43.5°C to be organized into fully condensed chro-matin. In addition it was not due to the failure of fully active topoisomerase II to be deposited at a normal frequency along replicating chromatin DNA. The failure of replicon clusters to be ligated into the genome resulted in the persistence of single, but not double, DNA strand breaks in the cell's genome 24 hours after cell heating.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcp.1041420220
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  • 7
    Electronic Resource
    Electronic Resource
    Preparation of diphenyl picrylhydrazyl (1955)
    Hoboken, NJ : Wiley-Blackwell
    Journal of Polymer Science 18 (1955), S. 141-143 
    Details
    ISSN: 0022-3832
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Physics
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/pol.1955.120188712
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  • 8
    Electronic Resource
    Electronic Resource
    Anti-integrin antibodies induce type IV collagenase expression in keratinocytes (1993)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 157 (1993), S. 190-200 
    Details
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: During wound healing, pericellular proteolysis is thought to be essential for the detachment of keratinocytes from basement membrane and in their migration into the wound bed. We have characterized integrin-type cell adhesion/migration receptors in human mucosal keratinocytes and examined their function in the regulation of type IV collagenase gene expression. Two major integrins of the β1 class, α2β1 and αβ1, were found to function as collagen and fibronectin receptors, respectively. Antibodies against β1 and α3 integrin subunits were found to stimulate the expression of the 92 kDa type IV collagenase severalfold in a dosedependent manner. Keratinocytes expressed also the 72 kDa type IV collagenase, the synthesis of which remained, however, unchanged in keratinocytes treated with anti-integrin antibodies. Stimulation of 92 kDa enzyme was found to be caused directly by antibody binding to integrins, since Fab-fragments of anti-β1 antibodies alone were able to induce collagenase expression in the absence of secondary, clustering antibodies. Antibodies against α2β1 integrin caused no stimulation. Keratinocytes seeded on different substrata (plastic, collagen, fibronectin, laminin, or vitronectin) showed equal induction of type IV collagenase expression. Expression of 92 kDa type IV collagenase could not be induced by peptides (GRGDS, GRGES), proteins (fibronectin, laminin, fibrinogen., albumin), or antibodies to fibronectin. We suggest that proteolytic processes around keratinocytes can be regulated by extracellular factors signalling through integrin-type receptors. © 1993 Wiley-Liss, Inc.
    Additional Material: 8 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcp.1041570125
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  • 9
    Electronic Resource
    Electronic Resource
    Large-scale computer simulation of fully developed turbulent channel flow with heat transfer (1991)
    Chichester : Wiley-Blackwell
    International Journal for Numerical Methods in Fluids 13 (1991), S. 999-1028 
    Details
    ISSN: 0271-2091
    Keywords: Turbulence ; Channel ; Heat transfer ; Spectral ; Numerical ; Simulation ; Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Recently, with the advent of supercomputers, there has been considerable interest in the use of direct numerical simulation to obtain information about turbulent shear flow at low Reynolds number. This paper presents a pseudospectral technique to solve the full three-dimensional time-dependent Navier-Stokes and advection-diffusion equations without the use of subgrid-scale modelling. The technique has not been previously used for fully developed turbulent channel flow simulation and is based on methods applied in other contexts. The emphasis of this paper is to provide a reasonably detailed account of how the simulation is done rather than to present new calculations of turbulence. The details of an algorithm for turbulent channel flow simulation and the grid and time step sizes needed to integrate through transient behaviour to steady state turbulence have not been published before and are presented here.Results from a Cray-2 simulation of fully developed turbulent flow in a channel with heat transfer are presented along with a critical comparison between experiment and computation. The first- and second-order moments agree well with experimental measurements; the agreement is poor for higher-order moments such as the skewness and flatness near the walls of the channel. Detailed information given about the effects of spatial grid resolution on a computed results is important for estimating the size of the computation required to study various aspects of a turbulent flow.
    Additional Material: 16 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/fld.1650130805
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  • 10
    Electronic Resource
    Electronic Resource
    The conformation of malformin A (1982)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Computational Chemistry 3 (1982), S. 89-94 
    Details
    ISSN: 0192-8651
    Keywords: Computational Chemistry and Molecular Modeling ; Biochemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Computer Science
    Notes: The conformation of the cyclic pentapeptide malformin A has been deduced by systematically generating possible models for the molecule, and minimizing the conformation energy of each. Only one of the low-energy solutions is fully consistent with the reported CD and NMR spectra, and this is the proposed model. The disulfide ring linking adjacent cystine residues is highly strained, as has been predicted from the S—S vibration frequency. The conformation of the backbone, but not that of the disulfide ring, is similar to a model previously proposed.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcc.540030114
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