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  • 1
    Electronic Resource
    Electronic Resource
    Radiation-induced decomposition and enzymatic hydrolysis of cellulose (1978)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 20 (1978), S. 1309-1315 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260200817
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  • 2
    Electronic Resource
    Electronic Resource
    Immobilization of Streptomyces phaerochromogenes by radiation-induced polymerization of glass-forming monomers (1979)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 21 (1979), S. 679-688 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Immobilization of Streptomyces phaerochromogenes was radiation induced polymerization of 2-hydroxylate at low temperatures. Radiation damage of the enzyme could be avoided by choosing irradiation at low temperatures. The enzymatic activity of immobilized cells increased remarkably with a decrease in the irradiation temperature of about -24°C. In constrast to the case of cell-free enzyme immobilization, the most characteristic case was that in these immobilized cells, the enzymatic activity did not decrease with repeated use even in the composite obtained at much lower monomer concentrations. Another characteristic of immobilized cells was the increase in enzymatic activity in the initial stage of repeated use, which could be attributed to the swelling effect of the polymer matrix, thereby increasing the enzymatic activity to whole cells.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260210412
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  • 3
    Electronic Resource
    Electronic Resource
    Enzyme immobilization by radiation-induced polymerization of hydrophobic glass-forming monomers at low temperatures (1979)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 21 (1979), S. 863-873 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Enzyme immobilization was studied by means of radiation-induced polymerization of hydrophobic glass-forming monomers at low temperatures. The polymerized hydrophobic composite was generally obtained in microspheric form. Enzymatic activity showed little decrease with repeated use in these systems. The particle size of the microsphere increased with increasing monomer concentration, and activity yield had a maximum at an optimum monomer concentration. Immobilization by copolymerization of hydrophilic and hydrophobic comonomers was also investigated and a maximum activity yield was found at a certain monomer concentration. A model scheme for immobilization at low temperatures was proposed and discussed.
    Additional Material: 8 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260210509
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  • 4
    Electronic Resource
    Electronic Resource
    Enzyme immobilization by radiation-induced polymerization of 2-hydroxyethyl methacrylate at low temperatures (1979)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 21 (1979), S. 847-861 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Enzyme immobilization by radiation-induced polymerization of hydrophilic glass forming monomers, such as 2-hydroxyethyl methacrylate, was studied. Enzyme radiation damage could be sufficiently retarded at low temperatures. The immobilized enzyme activity yield was markedly higher at low temperature than at higher temperature polymerization. At low temperatures the polymerized composite had a porous structure owing to ice crystallization which depends on the monomer concentration. It was deduced that the enzyme was partially trapped on the polymer surface, partially isolated in the pore, and partially occluded inside the polymer matrix. A decrease in activity caused by enzyme leakage was observed with repeated use in enzyme reactions where the composites had a large porosity. The activity yield showed a maximum at certain optimum porosities, i.e., at optimum monomer concentrations. Continuous enzyme reaction was preferably carried out using immobilized enzyme columns.
    Additional Material: 11 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260210508
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  • 5
    Electronic Resource
    Electronic Resource
    Enhancement of cellulase production by immobilization of Trichoderma reesei cells (1989)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 33 (1989), S. 1358-1362 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260331021
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  • 6
    Electronic Resource
    Electronic Resource
    Immobilization of microbial cells in membrane form by radiation-induced cast-polymerization (1983)
    Weinheim : Wiley-Blackwell
    Angewandte Makromolekulare Chemie 115 (1983), S. 75-86 
    Details
    ISSN: 0003-3146
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Physics
    Description / Table of Contents: Es wird über ein Verfahren zur Immobilisierung von Zellen mittels Strahlungs-Guß-Polymerisation berichtet. Die Immobilisierungsparameter, die die Enzymaktivität der immobilisierten Zellmembranen beeinflussen, wurden in absatzweisen Enzymreaktionen untersucht. Es handelte sich dabei um folgende Parameter: Monomerkonzentration, Membrandicke und Konzentration von Zusätzen. Die Michaelis-Konstante von immobilisierten Zellmembranen hängt von der Membrandicke und der Konzentration der Zusatzstoffe ab. Die Wärme- und pH-Stabilität der immobilisierten Zellmembaranen wurde untersucht.
    Notes: A method of cell immobilization by radiation-induced cast-polymerization is reported. Immobilization parameters which influence the enzymatic activity of immobilized cell membranes have been studied in batch enzyme reactions. These parameters are monomer concentration, membrane thickness and additive concentration. The Michaelis constant of immobilized cell membranes depends on the membrane thickness and additive concentration. The heat and pH stability of immobilized cell membranes was studied.
    Additional Material: 11 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/apmc.1983.051150107
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  • 7
    Electronic Resource
    Electronic Resource
    Synthesis and polymerization of (S)-2-methacryloyloxymethyl-1-(4-nitrophenyl)pyrrolidine (1989)
    Basel : Wiley-Blackwell
    Die Makromolekulare Chemie, Rapid Communications 10 (1989), S. 517-520 
    Details
    ISSN: 0173-2803
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Physics
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/marc.1989.030101003
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  • 8
    Electronic Resource
    Electronic Resource
    A new biodegradable polydepsipeptide consisting of (L-alanyl)n-L-lactyl sequences (n = 0, 1, 2, and 3) (1990)
    Basel : Wiley-Blackwell
    Die Makromolekulare Chemie, Rapid Communications 11 (1990), S. 337-343 
    Details
    ISSN: 0173-2803
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Physics
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/marc.1990.030110706
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  • 9
    Electronic Resource
    Electronic Resource
    Radiation polymerization of acrolein at low temperatures (1985)
    New York : Wiley-Blackwell
    Journal of Polymer Science: Polymer Chemistry Edition 23 (1985), S. 131-137 
    Details
    ISSN: 0360-6376
    Keywords: Physics ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Radiation polymerization of acrolein in the presence of the additives such as 2-hydroxyethyl methacrylate, acrylic acid, and water at low temperatures (0-78°C) was studied. The polyacrolein resulting from the polymerization gave a small particlelike microsphere (0.5-5 μm in diameter). The rate of the polymerization reaction of acrolein appeared to be accelerated by the addition of a small amount of 2-hydroxyethyl methacrylate or acrylic acid. The particle size of the polyacrolein particle varied with the condition of the polymerization and increased or decreased with an increase in the addition concentration of 2-hydroxyethyl methacrylate or acrylic acid.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/pol.1985.170230114
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  • 10
    Electronic Resource
    Electronic Resource
    Immobilization of microbial cells on cellulose-polymer surfaces by radiation polymerization (1983)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Applied Polymer Science 28 (1983), S. 3759-3765 
    Details
    ISSN: 0021-8995
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Streptomyces phaeochromogenes cells were immobilized on cellulose-polymer surfaces by radiation polymerization using hydrophilic monomers and paper. The enzyme activity of immobilized cell sheets was higher than that of immobilized cell composites obtained by the usual radiation polymerization technique. The enzyme activity of the sheets was affected by monomer concentration, the thickness of paper, and the degree of polymerization of paper. The copolymerization of hydroxyethyl methacrylate and methoxytetraethyleneglycol methacrylate in the sheets led to a further increase of the enzyme activity due to the increase of the hydrophilicity of the polymer matrix. The Michaelis constant of the sheets from low monomer concentration was close to that of intact cells.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/app.1983.070281216
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