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  • 1
    Electronic Resource
    Electronic Resource
    Viscoelastic properties of demineralized human dentin measured in water with atomic force microscope (AFM)-based indentation (1998)
    Hoboken, NJ : Wiley-Blackwell
    Journal of Biomedical Materials Research 40 (1998), S. 539-544 
    Details
    ISSN: 0021-9304
    Keywords: atomic force microscope (AFM) ; dentin ; collagen ; viscoelasticity ; mechanical properties ; elastic modulus ; Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine , Technology
    Notes: Using an atomic force microscope (AFM) with an attachment specifically designed for indentation, we measured the mechanical properties of demineralized human dentin under three conditions: in water, in air after desiccation, and in water after rehydration. The static elastic modulus (Ehr = 134 kPa) and viscoelastic responses (τε = 5.1 s and τσ = 6.6 s) of the hydrated, demineralized collagen scaffolding were determined from the standard linear solid model of viscoelasticity. No significant variation of these properties was observed with location. On desiccation, the samples showed considerably larger elastic moduli (2 GPa), and a hardness value of 0.2 GPa was measured. Upon rehydration the elastic modulus decreased but did not fully recover to the value prior to dehydration (381 kPa). © 1998 John Wiley & Sons, Inc. J Biomed Mater Res, 40, 539-544, 1998.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
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    Paper (German National Licenses)
  • 2
    Electronic Resource
    Electronic Resource
    Atomic force microscopy of conditioning agents on dentin (1995)
    Hoboken, NJ : Wiley-Blackwell
    Journal of Biomedical Materials Research 29 (1995), S. 1381-1387 
    Details
    ISSN: 0021-9304
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine , Technology
    Notes: Dentin conditioners provide a microporous surface for penetration by bonding agents. This study used an atomic force microscope (AFM) to examine the initial steps in the conditioning process of dentin using three demineralizing agents, 0.5 M EDTA, and dilute solutions of phosphoric (3mM, 6mM) and citric (5 mM) acids, in order to establish the relationships between demineralization and changes in surface morphology. Polished dentin disks had a 10-nm-thick gold pattern applied which served as a height reference. Samples(n = 3/agent) were examined at baseline and at 2-s intervals for up to 120 s for each agent. EDTA (0.5 M) was used as received; other conditioners were diluted to slow the rates of demineralization for detailed study. The surfaces of the peritubular and intertubular regions were altered differently. Initially subsidence rates were equal and linear, but after a 100-nm depth change the intertubular rates decreased. For phosphoric acid and citric acid, the movement of the intertubular surface was uniform and the surfaces remained smooth. However, the intertubular surfaces were rough for the EDTA treatment. The surface subsidence reached a plateau after a depth change of about 0.5μm, which resulted from a limit to the contraction of the demineralized and hydrated collagen scaffold. © 1995 John Wiley & Sons, Inc.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jbm.820291109
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  • 3
    Electronic Resource
    Electronic Resource
    Effect of citric acid concentration on dentin demineralization, dehydration, and rehydration: Atomic force microscopy study (1998)
    Hoboken, NJ : Wiley-Blackwell
    Journal of Biomedical Materials Research 42 (1998), S. 500-507 
    Details
    ISSN: 0021-9304
    Keywords: dentin ; demineralization ; shrinkage ; rehydration ; atomic force microscopy ; Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine , Technology
    Notes: Most current dentin bonding procedures use acid etchants to partially demineralize the dentin structure and provide pathways for resin infiltration. This study determined the recession rates of peritubular dentin and intertubular dentin as a function of pH during demineralization in citric acid solutions (0.0005-2.5M) and the effects of dehydration and rehydration on the partially demineralized dentin. Polished dentin disks were prepared with an internal reference layer and were studied at specific intervals for citric acid etching between pH 1 and 3.4 in an atomic force microscope. Peritubular dentin etched rapidly and linearly with time until it could no longer be measured. The intertubular surface began etching at nearly the same rate, but then recession slowed for all concentrations and stabilized after recession of less than 1 μm for all but the pH 1 solution. The decrease in recession was attributed to the limitation of contraction of the demineralized collagen scaffold as long as it remained hydrated. Dehydration following etching resulted in significant collapse of the surface, changes in roughness, and a slight decrease in tubule diameter for samples etched for 30 min. Measurements could not be made of the collapse for low pH samples, because shrinkage stresses disrupted the integrity of the reference layer. On rehydration, the dehydrated surfaces underwent an expansion up to the level seen after etching and tubule diameters returned to the etched values. These results indicate that the collapse of demineralized matrix is almost totally recoverable on rehydration. © 1998 John Wiley & Sons, Inc. J Biomed Mater Res, 42, 500-507, 1998.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
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