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  • 1
    Electronic Resource
    Electronic Resource
    Assessment of virus production and chloramphenicol acetyl transferase expression by insect cells in serum-free and serum-supplemented media using a temperature-sensitive baculovirus (1991)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 38 (1991), S. 1091-1099 
    Details
    ISSN: 0006-3592
    Keywords: chloramphenicol acetyl transferase ; baculovirus ; Spodoptera frugiperda ; serum-free medium ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Spodoptera frugiperda insect cells were grown in Sf-900 serum-free medium and two kinds of serum-supplemented media (IPL -41 and Grace's). The specific growth rates of uninfected cells were found to be 0.024, 0.35, and 0.034 h-1 respectively, at 33°C. The IPL -41 medium supported to highest maximum cell density (10.6 × 106 cells/mL) compared to 3.5 × 106 and 8.7 × 106 cells/mL with the Grace's and serum-free media, respectively. In temperature shifdown experiments with a temperature-sensitive baculo-virus (acts10YM1CAT), virus titer and chloramphenicol acetyl transferase (CAT) expression were highest in the IPL -41 (5.1 × 107 PFU/mL and 20000 U/mL). Use of Grace's medium gave higher virus titers than the serum-free medium (4.4 × 106 vs 4.1 × 105 PFU/mL) as well as higher CAT titers (7050 vs 1980 U/mL). Interestingly, in the three media used, the highest virus and CAT titers were obtained at MOI (multiplicity of infection) of 0.02 At MOI of 2.0 virtually no increase in virus of CAT titer was observed. This result is contrary to those obtained at constant-temperature (27°C) infection and cell culture, in which higher virus titers and recombinant protein expression and obtained at higher MOI.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260380918
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  • 2
    Electronic Resource
    Electronic Resource
    EXAFS and FTIR studies on the binding of deoxycholic acid with copper and zinc ions (1995)
    New York, NY [u.a.] : Wiley-Blackwell
    Biospectroscopy 1 (1995), S. 291-296 
    Details
    ISSN: 1075-4261
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: Deoxycholic acid is an important physiological bile acid. The binding of bile salts with metal ions has received more attention due to its clinical significance. In this study, a series of copper and zinc deoxycholate complexes were synthesized. EXAFS and FTIR spectroscopic techniques were used for characterization. The results indicated that both the carboxyl and hydroxyl groups of deoxycholic acid are binding to the Cu (or Zn) ion to form a new complex. There are six oxygen atoms surrounding the metal ion with the average bond lengths of Cu-O and Zn-O are 1.95 and 2.06 Å respectively. © 1995 John Wiley & Sons, Inc.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bspy.350010407
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  • 3
    Electronic Resource
    Electronic Resource
    Mono- and dilayer analogues of crystalline atomic hydrogen (1990)
    New York, NY : Wiley-Blackwell
    International Journal of Quantum Chemistry 38 (1990), S. 873-879 
    Details
    ISSN: 0020-7608
    Keywords: Computational Chemistry and Molecular Modeling ; Atomic, Molecular and Optical Physics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: As well as being of interest in their own right, many of the essential features of the lattice properties of crystalline atomic hydrogen should be interpretable in terms of the lattice properties of one- or two-layer hydrogen films. Here we present optimized equilibrium lattice parameters for monatomic hydrogen one- and two-layer films in several Bravais lattices as determined by all electron, full potential localdensity-approximation calculations.
    Additional Material: 4 Tab.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/qua.560382485
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  • 4
    Electronic Resource
    Electronic Resource
    Electronic stopping power for protons in an LiF monolayer (1993)
    New York, NY : Wiley-Blackwell
    International Journal of Quantum Chemistry 48 (1993), S. 219-226 
    Details
    ISSN: 0020-7608
    Keywords: Computational Chemistry and Molecular Modeling ; Atomic, Molecular and Optical Physics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: In our previous work, the stopping properties of metallic and covalent films were investigated. Here we consider an ionically bound film. The energy loss of a proton in an LiF monolayer (LiF-1L) is calculated in orbital fashion, based on kinetic theory. The required momentum density and mean excitation energy are obtained from the local density approximation and local plasma approximation respectively. For comparison, the LiF molecule is treated by use of a large intermolecular distance in the film. We find the stopping cross section of the LiF molecule to be only slightly larger than that for the LiF-1L. The Bragg rule (additivity of stopping for the corresponding atoms) is not valid for the ionically bound molecule nor the corresponding extended system, but may be valid if additivity of stopping of atomic ions is assumed. © 1993 John Wiley & Sons, Inc.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/qua.560480824
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  • 5
    Electronic Resource
    Electronic Resource
    Fundamental solutions and numerical methods for flow problemsThis invited paper is an extended, and refereed version of one presented at the Fourth International Symposium on Finite Elements in Flow Problems held in Tokyo, Japan, 26-29 July 1982. (1984)
    Chichester : Wiley-Blackwell
    International Journal for Numerical Methods in Fluids 4 (1984), S. 185-201 
    Details
    ISSN: 0271-2091
    Keywords: Potential Flows ; Navier-Stokes Problems ; Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: An approach for the numerical solution of flow problems based on the concept of fundamental solutions of differential equations is described. This approach uses the finite element methodology but does not rely on the concept of variational principle or that of residuals. The approach is shown to be well-suited for many types of flow problems. Various applications of this approach are discussed in this paper, with particular emphasis placed on the solution of potential flows and viscous flows containing appreciable regions of separation.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/fld.1650040207
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  • 6
    Electronic Resource
    Electronic Resource
    Fatigue behavior of quasi-isotropic carbon fiber/PEEK laminates under tension-tension loading (1995)
    Brookfield, Conn. : Wiley-Blackwell
    Polymer Composites 16 (1995), S. 215-223 
    Details
    ISSN: 0272-8397
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Fatigue behavior of carbon fiber reinforced poly(etheretherketone)(PEEK) laminates was investigated. The static tensile measurement, tension-tension fatigue loading tests, and residual tensile strength measurement of the [0/45/90/-45]2s AS-4/PEEK laminates were performed at various levels of stress amplitudes. The influences of stress amplitude on the fatigue life and the residual tensile strength were investigated. The experimental results for fatigue life and residual tensile strength under different stress amplitudes are analyzed by the median rank method. The S-N curves at various survival probabilities are also presented by the pooled Weibull distribution function. Furthermore, a residual strength degradation model is used to predict the residual strength for the composites subjected to a number of fatigue cycles and to simulate the effects of the stress amplitude on the fatigue life. The agreement between experiment and theory is good.
    Additional Material: 17 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/pc.750160305
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  • 7
    Electronic Resource
    Electronic Resource
    Surface composition of machined leaded brass (1994)
    Chichester [u.a.] : Wiley-Blackwell
    Surface and Interface Analysis 22 (1994), S. 323-326 
    Details
    ISSN: 0142-2421
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Physics
    Notes: ESCA and RBS were used to obtain quantitative information on the thickness and nature of the surface layers formed on machined brasses containing 1-3% lead. The results show that the brass surface is strongly enriched in lead. The amount of this element accumulated on the surface increases with increasing bulk concentration. Lead appears to be present as islands partially covering the brass surface and having variable thicknesses up to several hundred Å. The outermost layer of the islands is mainly a Pb hydroxycarbonate, under which PbO and Pb metal can be detected. Between the islands, the brass surface is covered by a layer of zinc oxide covering the Cu—Zn metallic phase. The accumulation of Pb at the surface is attributable to a smearing effect caused by the machining cool.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/sia.740220170
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  • 8
    Electronic Resource
    Electronic Resource
    The influence of normal flow boundary conditions on spurious modes in finite element solutions to the shallow water equations (1994)
    Chichester : Wiley-Blackwell
    International Journal for Numerical Methods in Fluids 18 (1994), S. 1021-1060 
    Details
    ISSN: 0271-2091
    Keywords: Finite elements ; Shallow water equations ; Boundary conditions ; Dispersion analysis ; Spurious modes ; Wave equation ; Primitive equations ; Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Finite element solutions of the primitive equation (PE) form of the shallow water equations are notorious for the severe spurious 2Δx modes which appear. Wave equation (WE) solutions do not exhibit these numerical modes. In this paper we show that the severe spurious modes in PE solutions are strongly influenced by essential normal flow boundary conditions in the coupled continuity-momentum system of equations. This is demonstrated through numerical examples that avoid the use of essential normal flow boundary conditions either by specifying elevation values over the entire boundary or by implementing natural flow boundary conditions in the weak weighted residual form of the continuity equation. Results from a series of convergence tests show that PE solutions are of nearly the same quality as WE solutions when spurious modes are suppressed by alternative specification of the boundary conditions. Network intercomparisons indicate that varying nodal support does not excite spurious modes in a solution, although it does enhance the spurious modes introduced when an essential normal flow boundary condition is used.Dispersion analysis of discrete equations for interior and boundary nodes offers an explanation of the observed solution behaviour. For certain PE algorithms a mixed situation can arise where the boundary nodes exhibit a monotonic (noise-free) dispersion relationship and the interior nodes exhibit a folded (noisy) dispersion relationship. We have found that the mixed situation occurs when all boundary nodes are specified elevation nodes (which are enforced as essential conditions in the continuity equation) or when specified flow boundary nodes are treated as natural boundary conditions in the continuity equation. In either case the effect is to generate a solution that is essentially free of noise. Apparently, the monotonic dispersion behaviour at the boundaries suppresses the otherwise noisy behaviour caused by the folded dispersion relation on the interior.
    Additional Material: 18 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/fld.1650181103
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  • 9
    Electronic Resource
    Electronic Resource
    Acrylic latices stabilized by polymerizable non-ionic surfactant (1996)
    New York, NY [u.a.] : Wiley-Blackwell
    Polymer International 40 (1996), S. 129-139 
    Details
    ISSN: 0959-8103
    Keywords: polymerizable non-ionic surfactant ; acrylic latices ; particle size ; bridging flocculation ; colloidal stability ; Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: The final latex particle size is controlled by the concentration of polymerizable non-ionic surfactant NE-40 in the emulsion copolymerization of methyl methacrylate (MMA) and butyl acrylate (BA). The particle size decreases with increasing NE-40 concentration and increases with increasing persulphate initiator concentration. The dependence of particle size on the initiator concentration does not follow conventional Smith-Ewart theory, which is attributed to the bridging flocculation process during the particle nucleation period. The differences in the particle nucleation and growth stages and colloidal stability observed in the NE-40 and nonyl phenol-40 mol ethylene oxide adduct (NP-40) stabilized systems can be attributed to the different distribution patterns of surfactant molecules in the particles. Experimental data also indicate that the particle size decreases with increasing electrolyte concentration, or agitation speed. The total scrap, presumably caused by the bridging flocculation process, increases rapidly with increase in the NaCl concentration The amount of large flocs formed during polymerization is generally greater for the run operated at higher agitation speed. As expected, the latex products stabilized by non-ionic surfactants show excellent stability toward added sodium salt.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
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  • 10
    Electronic Resource
    Electronic Resource
    Incompressibility without tears - HOW to avoid restrictions of mixed formulation (1991)
    Chichester [u.a.] : Wiley-Blackwell
    International Journal for Numerical Methods in Engineering 32 (1991), S. 1189-1203 
    Details
    ISSN: 0029-5981
    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mathematics , Technology
    Notes: The severe restrictions of the Babuška-Brezzi stability criteria permit only a limited number of interpolations for velocities (displacements) and pressure to be used for incompressible behaviour. These restrictions preclude the use of many useful elements. We show in this paper how such difficulties can be side-stepped by seeking the steady state solution through the use of various time marching schemes. This permits a simple iterative approach to incompressible (or nearly incompressible) problems of fluid mechanics or solid mechanics and provides a stimulus for the use of such procedures in metal forming flow, etc.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/nme.1620320603
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