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  • Wiley-Blackwell  (10)
  • 1
    Electronic Resource
    Electronic Resource
    Hoboken, NJ : Wiley-Blackwell
    AIChE Journal 39 (1993), S. 1421-1430 
    ISSN: 0001-1541
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The influence of shear thinning and viscoelasticity on the power required for the mixing of viscous liquids using six different helical ribbon agitators has been investigated. Four Newtonian and 12 non-Newtonian fluids prepared using several polymers dissolved in varying concentrations in different solvents cover a wide range of rheological properties. By a careful choice of test media, the specific and combined effects of shear thinning and viscoelasticity on the power requirement have been examined. Simple models are proposed to predict the effective shear rate in the tank from the knowledge of the torque or power number. The effective shear rate predictions compared with the effective shear rate estimated using the scheme of Metzner and Otto (1957) show that they slightly depend on the shear thinning properties. Fluid's elasticity increases appreciably the power requirement, and departures from the generalized Newtonian power curve in the laminar regime are observed at smaller Reynolds numbers for viscoelastic fluids. Bottom wall resistance of the mixing vessel makes a negligible contribution to the power consumption.
    Additional Material: 14 Ill.
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  • 2
    ISSN: 1040-0397
    Keywords: Rotating disk electrode voltammetry/anodic stripping voltammetry ; Nafion-coated thin mercury film electrode ; Chemical speciation of lead and cadmium complexes ; River surface waters ; Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Rotating disk electrode voltammetry (RDEV) in conjunction with anodic stripping voltammetry (ASV) with a thin mercury film electrode (TMFE) or with a Nafion-coated thin mercury film electrode (NCTMFE) has been evaluated for the direct determination (i.e., without preconcentration) of lead and cadmium speciation in aqueous solutions containing dissolved organic matter. The RDEV technique has been used to estimate dissociation rate constants and diffusion coefficients; pseudopolarograms have indicated the identity of the metal complexes undergoing electrode reductions at the corresponding plateaus of the pseudopolarograms in a number of model solutions containing Cd or Pb and known amounts of fulvic acid. It has been found that the effect of dissolved organic material is measurable when the concentrations of fulvic acid are equal to or less than 20 μg/mL (which is below the concentration of naturally occurring dissolved organic carbon in most freshwaters). The effective electrode surface area has been found to be considerably less than the geometric surface area, and if this fact is not explicitly taken into consideration, the result would be an erroneous estimation of dissociation rate constants and diffusion coefficients. Pseudopolarograms of aqueous samples containing Cd or Pb and fulvic acid and samples of Rideau river surface water clearly show that NCTMFEs effectively block the transport of large inert metal-FA complexes to the electrode surface, resulting in interference-free determination, and giving well-defined pseudopolarograms, and yet do not hinder the transport of small metal-FA complexes to the electrode surface. It is concluded that RDEV/ASV offers promise of being a valuable technique for determining chemical speciation of lead and cadmium in river surface waters.
    Additional Material: 12 Ill.
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  • 3
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Applied Organometallic Chemistry 12 (1998), S. 201-220 
    ISSN: 0268-2605
    Keywords: MOCVD ; molybdenum oxycarbide ; chromium oxycarbide ; coating ; kinetics ; microstructure ; Chemistry ; Industrial Chemistry and Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: A review of the coating kinetics of molybdenum and chromium oxycarbides and a study on the properties of deposited films are reported. Molybdenum carbonyl, chromium carbonyl and their mixture were used to prepare coatings at temperatures between 170 and 450 °C on SS304 or SiC substrates by a process of metal-organic chemical vapor deposition (MOCVD). The processing parameters of the coatings, such as the evaporation of precursors, coating pressure and temperature, are discussed along with the coating rates, compositions and other microstructural information, so to reveal the kinetics of the coating process on two different substrates. In addition, the chemical composition, crystalline phases and microstructure of the coatings obtained in various conditions are presented with the evidence interpreted by various electron-microscopic and spectroscopic techniques, including XRD, EDS, EPMA and XPS. The dependence of some properties, such as hardness, adhesive strength and corrosive resistance of the coatings on SS 304, on their composition and the deposited phases, i.e. as a function of coating temperature and pressure, is reviewed. © 1998 John Wiley & Sons, Ltd.
    Additional Material: 14 Ill.
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  • 4
    Electronic Resource
    Electronic Resource
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 12 (1989), S. 258-260 
    ISSN: 0935-6304
    Keywords: High performance affinity chromatography ; Fast affinity chromatography ; Heparin ; Bile salt stimulated lipase (BSSL) ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Additional Material: 3 Ill.
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  • 5
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 128 (1986), S. 475-484 
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The ability of heparin or that of hexuronyl hexosaminoglygan sulfate (HHS-4) to protect basic or acidic fibroblast growth factor (FGF) from acid or heat inactivation has been analyzed. Both freshly prepared basic and acidic FGF stimulate the growth of baby hamster kidney (BHK-21) cells exposed to medium supplemented with transferrin and insulin. Freshly prepared basic FGF was 10 fold more potent than acidic FGF. The addition of heparin to the medium decreased the potency of basic FGF while it potentiated that of acidic FGF. Upon storage of FGF at -80°C, a decline in potency of both basic and acidic FGF was observed. Heparin, when added to the medium, potentiated their activities, which became similar to that of freshly prepared basic FGF. In order to test whether heparin could protect basic or acidic FGF from inactivation, both mitogens were exposed to acid conditions (1% trifluoroacetic acid, pH 1.08, 2 h) or heat (65°C, 5 min) which inactivate basic or acidic FGF. When exposed to such treatment in the presence of heparin or HHS-4, basic and acidic FGF retained their potency. The effect of heparin and HHS-4 on the bioactivity of basic and acidic FGF is truly of a protective nature, since they had no effect when added after inactivation of the mitogens. Potentiation of the bioactivity of the protected mitogens or of the inactivated one could only be observed when cells were exposed to high heparin or HHS-4 concentrations. This indicates that heparin and HHS-4, in addition to protecting FGF from inactivation, also acts at another locus, as yet unidentified.
    Additional Material: 10 Ill.
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  • 6
    Electronic Resource
    Electronic Resource
    Chichester [u.a.] : Wiley-Blackwell
    International Journal for Numerical Methods in Engineering 39 (1996), S. 1115-1136 
    ISSN: 0029-5981
    Keywords: particle tracking ; Lagrangian-Eulerian finite element methods ; Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mathematics , Technology
    Notes: This paper presents a multi-dimensional particle tracking technique for applying the Lagrangian-Eulerian finite element method to solve transport equations in transient-state simulations. In the Lagrangian- Eulerian approach, the advection term is handled in the Lagrangian step so that the associated numerical errors can be considerably reduced. It is important to have an adequate particle tracking technique for computing advection accurately in the Lagrangian step. The particle tracking technique presented here is designed to trace fictitious particles in the real-world flow field where the flow velocity is either measured or computed at a limited number of locations. The technique, named ‘in-element’ particle tracking, traces fictitious particles on an element-by-element basis. Given a velocity field, a fictitious particle is traced one element by one element until either a boundary is encountered or the available time is completely consumed. For the tracking within an element, the element is divided into a desired number of subelements with the interpolated velocity computed at all nodes of the subelements. A fictitious particle, thus, is traced one subelement by one subelement within the element. The desired number of subelements can be determined based on the complexity of the flow field being considered. The more complicated the flow field is, the more subelements are needed to achieve accurate particle tracking results. A single-velocity approach can be used to efficiently perform particle tracking in a smooth flow field, while an average-velocity approach can be employed to increase the tracking accuracy for more complex flow fields.
    Additional Material: 15 Ill.
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  • 7
    Electronic Resource
    Electronic Resource
    Chichester [u.a.] : Wiley-Blackwell
    International Journal for Numerical Methods in Engineering 39 (1996), S. 987-1016 
    ISSN: 0029-5981
    Keywords: Lagrangian-Eulerian methods ; advection-diffusion transport equations ; adaptive local zooming ; peak/valley capturing ; slave point ; Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mathematics , Technology
    Notes: A Lagrangian-Eulerian method with adaptively local ZOOMing and Peak/valley Capturing approach (LEZOOMPC), consisting of advection-diffusion decoupling, backward particle tracking, forward particle tracking, adaptively local zooming, peak/valley capturing and slave point utilization, is presented to solve two-dimensional advection-diffusion transport equations. This approach and the associated computer code, 2DLEZOOMPC, were developed to circumvent the difficulties associated with the EPCOF scheme, developed earlier by the authors, when it was extended from a one-dimensional space to a multi- dimensional space. In EPCOF, all the nodes, including global nodes and fine-grid nodes, of the previous time are forward tracked for both determining rough elements and exactly capturing peaks and valleys. After kicking off those unnecessary nodes, a subset of the forward-tracked nodes are activated to preserve the shape of spatial distribution of the quantity of interest (e.g. concentration in the solute transport). The accurate results of applying EPCOF to solving two one-dimensional bench-mark problems under a variety of conditions have shown the capability of this scheme to eliminate all types of numerical errors associated with the advection term and to keep the maximum computational error to be within the prescribed error tolerance. However, difficulties arose when the EPCOF scheme was extended to a multi-dimensional space mainly due to the geometric difference between a one-dimensional space and a multi-dimensional space. To avoid these geometric difficulties, we modified the EPCOF scheme and named the modified scheme LEZOOMPC. LEZOOMPC uses regularly local zooming for rough elements and peak/valley capturing within subelements to resolve the problems of triangulation and boundary source as well as to preserve the shape of concentration distribution. In addition, LEZOOMPC employs the concept of slave points to deal with the compatibility problem associated with the diffusion zooming in a multi-dimensional space. As a result, not only is the geometrical problem resolved, but also the spirit of EPCOF is retained. Application of 2DLEZOOMPC to solving three two-dimensional bench-mark problems indicates it yields extremely accurate results for all the test cases. 2DLEZOOMPC could solve advection-diffusion transport problems accurately to within any prescribed error tolerance by using mesh Peclet numbers ranging from 0 to ∞ and very large time-step sizes as well as coarse global grid sizes. The size of time-step is related to both the diffusion coefficients and mesh sizes. Hence, it is limited only by the diffusion solver. Extension of this approach to a three-dimensional space will contain only implementation complexity but neither conceptual nor implementation difficulties. Details of the three-dimensional computer code, 3DLEZOOMPC, is to be presented in the companion paper.
    Additional Material: 17 Ill.
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  • 8
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 110 (1982), S. 72-80 
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Experimental conditions have been defined that allow bovine corneal endothelial (BCE) cells to grow in the complete absence of serum. Low density BCE cell cultures maintained on extracellular matrix (ECM)-coated dishes and plated in the total absence of serum proliferate actively when exposed to a synthetic medium supplemented with high density lipoprotein (HDL 500 μg protein/ml), transferrin (10 μg/ml), insulin (5 μg/ml), and fibroblast (FGP) or epidermal growth factor (EGF) added at concentrations of 100 or 50 ng/ml, respectively. Omission of any of these components results in a lower growth rate and/or final cell density of the cultures. BCE cell cultures plated on plastic dishes and exposed to the same synthetic medium grow very poorly. The longevity of BCE cultures maintained on plastic versus ECM and exposed to serum-free versus serum-containing medium has been studied. The use of ECM-coated dishes extended the life span of BCE cultures maintained in serum-supplemented medium to over 120 generations, as compared to less than 20 generations for cultures maintained on plastic. Likewise, BCE cells maintained on ECM and exposed to a synthetic medium supplemented with optimal concentrations of HDL, transferrin, insulin, and FGF underwent 85 generations, whereas control cultures maintained on plastic could not be passaged. The enhancing effect of ECM on BCE cell growth and culture longevity clearly illustrates the importance of the cell substrate in the control of proliferation of these cells.
    Additional Material: 5 Ill.
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  • 9
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 114 (1983), S. 267-278 
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Liposomes made by sonication of egg yolk phosphatidyl choline support the proliferation of low-density bovine vascular and corneal endothelial cells, and vascular smooth muscle cells maintained on basement laminacoated dishes and exposed to a defined medium supplemented with transferrin. The optimal growth-promoting effect of phosphatidyl choline was observed at concentrations of 25 μg/ml for low-density cultures of vascular smooth muscle cells, and 100 μg/ml for vascular and corneal endothelial cells. The growth rate and final cell density of vascular endothelial cells exposed to a synthetic medium supplemented with transferrin and either high-density lipoproteins or phosphatidyl choline has been compared. Although cultures exposed to phosphatidyl choline reached a final cell density similar to that of cultures exposed to high-density lipoproteins, they had a longer average doubling time (17 h vs. 12 h) during their logarithmic growth phase and a shorter lifespan (17 generations vs. 30 generations). Similar observations were made in the case of vascular smooth muscle cells or bovine corneal endothelial cells maintained in medium supplemented with transferrin, fibroblast growth factor (FGF) or epidermal growth factor (EGF), and insulin and exposed to either high-density lipoproteins or phosphatidyl choline. Since phosphatidyl choline can, for the most part, replace highdensity lipoproteins in supporting the proliferation of various cell types, it is likely that the growth stimulating signal conveyed by high-density lipoproteins is associated with its polar lipid fraction, which is composed mostly of phosphatidyl cholines.
    Additional Material: 9 Ill.
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  • 10
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Bovine adrenal and brain cortex and corpus luteum-derived capillary endothelial cells have been established in culture, taking advantage of their ability to proliferate at clonal density when maintained on extracellular matrix (ECM) coated dishes in the presence of serum supplemented medium. All three cell types formed at confluency a monolayer of small, tightly packed, contact inhibited cells that express factor VIII related antigen. Their proliferative response to basic and acidic FGF when cells were maintained on plastic and exposed to serum supplemented medium was similar to that previously reported for endothelial cells derived from large vessels, with acidic FGF being 30-fold less potent than basic FGF. Their requirement for high density lipoproteins and transferrin in order to proliferate actively when maintained on ECM-coated dishes and exposed to serum-free conditions was also similar to that previously reported for endothelial cells derived from large vessels. Heparin strongly reduced the proliferative response of capillary endothelial cells to either basic or acidic FGF, as well as their response to serum alone, regardless of whether cells were maintained on plastic or on ECM-coated dishes. The present data indicate that bovine endothelial cells derived from large or small vessels are indistinguishable in so far as their response to growth factors, plasma factors, and substrata are concerned.
    Additional Material: 11 Ill.
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