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Some water-insoluble papain derivatives (1966)

Silman, Israel H. ; Albu-Weissenberg, Martin ; Katchalski, Ephraim

New York : Wiley-Blackwell

Biopolymers 4 (1966), S. 441-448

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ISSN: 0006-3525

Keywords: Chemistry ; Polymer and Materials Science

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Chemistry and Pharmacology

Notes: A description is given of the preparation and properties of four water-insoluble papain derivatives by three different techniques: (a) covalent binding to a water-insoluble diazoimitn salt derived from a copolymer of p-aminophenylalanine and leucine; (b) binding to insoluble preparations of collagen via bisdiazobenzidine-2,2′-disulfonic acid; (c) in-solubilization by crosslinking the enzyme itself with bisdiazobenzidine. By all three methods it, was possible to obtain stable water-insoluble papain derivatives retaining much of the activity of the native enzyme on low and high molecular weight substrates.

Additional Material: 2 Ill.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1002/bip.1966.360040405

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