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  • 1
    Electronic Resource
    Electronic Resource
    New York, NY : Wiley-Blackwell
    Journal of Electron Microscopy Technique 4 (1986), S. 371-379 
    ISSN: 0741-0581
    Keywords: Scanning electron microscopy ; Low temperature ; Microanalysis ; Duodenum ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Natural Sciences in General
    Notes: Low temperature scanning electron microscopy of mammalian tissue allows microanalysis data and morphological results to be considered together, without the risk of elemental diffusion introduced by liquid fixatives and processing agents. The technique, however, is time consuming and there are many areas where errors are possible and standardization of technique is advisable. The present paper describes some of the problems inherent in handling frozen tissue and comparison is made between microanalytical measurements from luminal contents and those from villous epithelium.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    New York, NY : Wiley-Blackwell
    Journal of Electron Microscopy Technique 5 (1987), S. 65-74 
    ISSN: 0741-0581
    Keywords: Scanning electron microscopy ; Low temperature ; Microanalysis ; Duodenum ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Natural Sciences in General
    Notes: Microanalysis data obtained using low temperature scanning electron microscopy of frozen hydrated etched mouse duodenum gives information about peak/background ratios of various elements in different cellular compartments. The present paper describes ways in which the data can be analyzed to minimize artifactual variations and to make clear where there are genuine differences in peak/background ratios when readings from different animals are compared. Using these methods, it is shown that in some areas of villous epithelium there are measurable differences in the ratios of sodium, calcium, and sulphur.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
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