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  • Wiley-Blackwell  (199)
  • 1
    Electronic Resource
    Electronic Resource
    Recent advances in research on fibronectin and other cell attachment proteins (1985)
    New York, N.Y. : Wiley-Blackwell
    Journal of Cellular Biochemistry 28 (1985), S. 79-97 
    Details
    ISSN: 0730-2312
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Fibronectin and other cell attachment proteins provide molecular models for beginning to unravel the complex interactions of the cell surface with the extracellular matrix. This area has been reviewed in considerable detail previously [1-10]. Our brief review will therefore be selective rather than comprehensive, and it will focus on some recent generalizations about this class of proteins, as well as on recent advances in the molecular analysis of the functions of these proteins and their receptors. we shall also present various popular or provocative hypotheses and speculations about future work in the field.
    Additional Material: 11 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcb.240280202
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  • 2
    Electronic Resource
    Electronic Resource
    Characterization of a membrane-associated glycoprotein complex implicated in cell adhesion to fibronectin (1985)
    New York, N.Y. : Wiley-Blackwell
    Journal of Cellular Biochemistry 28 (1985), S. 307-318 
    Details
    ISSN: 0730-2312
    Keywords: glycoprotein complex ; cell adhesion ; fibronectin ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: We have characterized a 140-kDa glycoprotein complex purified by a monoclonal antibody and implicated in cell adhesion to the extracellular molecule fibronectin. Three major polypeptide components were purified by monoclonal antibody JG22E, which had apparent molecular weights of 155,000 (band 1), 135,000 (band 2), and 120,000 (band 3). In two-dimensional gel electrophoresis, each subunit migrated as either a broad band or a series of spots at acidic isoelectric points. After treatment with neuraminidase, the spots became focused around pH 6.2 (band 1), pH 5.6 (band 2), and pH 5.3 (band 3). These three major bands were compared by two-dimensional peptide mapping in a series of pairwise combinations and were found to be distinct proteins. In sucrose gradients, these proteins co-migrated as a complex sedimenting at approximately 8.4 S either before or after affinity purification, whereas separated subunits migrated at 4.7 to 5.8 S. Amino acid analysis revealed no detectable hydroxyproline and a composition characterized by a substantial number of cysteine residues compared to the average protein. Our results suggest that a noncovalent complex of structurally distinct glycoproteins is involved in adhesive interactions of fibronectin with cells.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcb.240280409
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  • 3
    Electronic Resource
    Electronic Resource
    Wavelength and depth of ribbing in roll coating and its elimination (1993)
    Hoboken, NJ : Wiley-Blackwell
    AIChE Journal 39 (1993), S. 935-945 
    Details
    ISSN: 0001-1541
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Experiment has been made on ribbing induced on a film coated over a roll surface by a two-roll system: one roll rotated with a speed and the other fixed or rotated with relatively different speeds. The two rolls have the same diameters and are placed parallel with each other by keeping a gap. Test fluids are Newtonian (glycerin/water) and viscoelastic [polyacrylamide (Separan AP30)/glycerin/water] solutions. Wavelength and depth of ribbing are measured by a newly developed technique utilizing an image of a straight bar placed above the roll and reflected on the ribbing film surface. Nondimensionalized wavelength is correlated with capillary number for Newtonian liquids, but higher for dilute Separan solutions and lower for nondilute Separan solutions than for Newtonian liquids. The depth of ribbing increases for dilute Separan solutions with nearly the same value for nondilute Separan solutions as those for Newtonian liquid. This behavior observed for the ribbing seems to have some relation to the existence of a line of vortices that occurs visibly for nondilute Separan solutions in the entrance region of the gap between the rolls. Increase in the ratio of the velocities of two rolls has an effect of increasing both the wavelength and depth of ribbing for glycerine and nondilute Separan solutions, but it has the effect of decreasing them for dilute Separan solutions. Addition of Separan in glycerine solution promotes the generation of ribbing.A string spanned near over the gap to touch the surface of the liquid pool between the gap has the effect of eliminating the ribbing. This is successful in various combinations between the speeds of the two rolls and the diameters of the string.
    Additional Material: 21 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/aic.690390603
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  • 4
    Electronic Resource
    Electronic Resource
    Similarity rule between heat transfer and pressure drop of porous materials (1992)
    Hoboken, NJ : Wiley-Blackwell
    AIChE Journal 38 (1992), S. 1840-1842 
    Details
    ISSN: 0001-1541
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/aic.690381115
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  • 5
    Electronic Resource
    Electronic Resource
    Stimulation of in vitro motility of Chlamydomonas axonemes by inhibition of cAMP-dependent phosphorylation (1987)
    New York, NY : Wiley-Blackwell
    Cell Motility and the Cytoskeleton 8 (1987), S. 302-311 
    Details
    ISSN: 0886-1544
    Keywords: flagella ; cAMP ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: When demembranted axonemes of Chlamydomonas were reactivated with Mg ATP, the proportion of motile axonemes was significantly increased by the preence of either phosphodiesterase (PDE) or protein inhibitor of cAMP-dependent kinase (PKI). The effect of PDE was cancelled by the addition of cAMP. These findings strongly suggest that the axoneme samples have endogenous cAMP, which can reduce the proportion of motile axonemes via phosphorylation. This inhibitory effect of cAMP on Chlamydomonas axonemes is opposite to its stimulatory effect on the axonemal motility in other organisms so far reported. PKI or PDE activated the motility motility either in the absence of Ca2+, when the axonemes beat with an asymmetric waveform, or in 10-5M Ca2+, when the axonemes beat with a symmetric waveform. This cAMP-dependent regulation of motility was observed with the axonemes from which detergent-soluble material had been removed, indicating that the proteins responsible for the regulation still remained in the axonemes. Preliminary in vitro phosphorylation stdies have implicated two polypetides as candidates for the target protein of cAMP-dependent protein kinase: one with a molecular weight of 270 kD and the other with a much larger molecular weight.
    Additional Material: 8 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/cm.970080403
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  • 6
    Electronic Resource
    Electronic Resource
    Stereoselective pharmacokinetics of [14C]-labeled KE-298, a new anti-rheumatic drug, in rats (1996)
    New York, NY [u.a.] : Wiley-Blackwell
    Chirality 8 (1996), S. 258-263 
    Details
    ISSN: 0899-0042
    Keywords: stereoselectivity ; radioactivity ; distribution ; hepatocytes ; metabolism ; protein binding ; analbuminemic rats ; Chemistry ; Organic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The stereoselective pharmacokinetics of two enantiomers of [14C]-labeled KE-298 [2-acetylthiomethyl-4-(4-methylphenyl)-4-oxobutanonic acid] were investigated in rats. The blood levels of radioactivity after the oral administration of (+)-(S)-[14C]KE-298 were higher than that for (-)-(R)-[14C]KE-298; the AUC of the former was approximately twice that of the latter. No significant stereoselectivity was observed in absorption rate. The tissue/plasma level ratios at 30 min after oral administration of (-)-(R)-[14C]KE-298 in the liver and kidney, the major metabolic and/or excretory organs, were 2 to 3 times higher than those for (+)-(S)-[14C]KE-298. Neither was evidence of stereoselectivity found in the excretion of radioactivity. During incubation with isolated rat hepatocytes in vitro, the metabolic rates of KE-298 enantiomers were not significantly different. Plasma protein binding 30 min after the oral administration of (+)-(S)-[14C]KE-298 and (-)-(R)-[14C]KE-298 was 99.3% and 97.0%, respectively. Comparing the unbound fraction, (-)-(R)-[14C]KE-298 was approximately 4 times higher than (+)-(S)-[14C]KE-298. In order to make clear the relationship between stereoselective pharmacokinetics and protein binding for [14C]KE-298, the comparative pharmacokinetics of (+)-(S)-[14C]KE-298 and (-)-(R)-[14CC]KE-298 were investigated in analbuminemic rats. In these animals, no evidence of stereoselectivity was found for either blood level-time profiles or plasma protein binding. These results revealed that the stereoselective pharmacokinetics of KE-298 in rats might be due to enantiomeric differences in binding to plasma albumin. © 1996 Wiley-Liss, Inc.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    Network formation and swelling behavior of photosensitive poly(vinyl alcohol) gels prepared by photogenerated crosslinkingPoster presented at the “International Symposium on Advanced Network-Polymers, Kansai University” (ISANKU), at Kansai University, Suita, Osaka, Japan, on December 5-6, 1995. (1996)
    Weinheim : Wiley-Blackwell
    Angewandte Makromolekulare Chemie 240 (1996), S. 231-239 
    Details
    ISSN: 0003-3146
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Physics
    Notes: Poly (vinyl alcohol) with pendent styrylpyridinium groups (SbQ) is insolubilized by photoirradiation. An association takes place in SbQ groups. The association of polymer chains becomes marked with increasing the number of SbQ groups. Mainly intermolecular crosslinks were formed. Transparent and homogeneous macrogels consisting of several intermolecular crosslinks are obtained.The proportion of the free water to the bound water in PVA-SbQ gels was 3.3-2.9 despite of the large change in conversion of photodimerization of SbQ groups, x=0.27-0.58. The water uptake after swelling of the gels in water increased 6-27 times compared to the original weight at pH=7. The higher the degree of photocrosslinking, the lower was the degree of swelling. The water diffusion coefficients, D, were (2.2-5.8) × 10-5 cm2 S-1 for a 88% saponified PVA with 1 . 3 mol% SbQ groups. The volume of the gel increased discontinuously about 10-fold for the 99% saponified PVA with 0 . 096 mol% SbQ and 51% water (49% acetone). The acetone concentration at the transition decreased with increasing the degree of saponification of the PVA.
    Additional Material: 10 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/apmc.1996.052400122
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  • 8
    Electronic Resource
    Electronic Resource
    Spontaneous molecular orientation of polyimides induced by thermal imidization. I. Uniaxial stretching of polyamic acid film (1994)
    Bognor Regis [u.a.] : Wiley-Blackwell
    Journal of Polymer Science Part B: Polymer Physics 32 (1994), S. 1299-1303 
    Details
    ISSN: 0887-6266
    Keywords: polyimides ; spontaneous molecular orientation ; uniaxial drawing ; absorption dichroism ; perylenebisimide dye ; Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Physics
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/polb.1994.090320716
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  • 9
    Electronic Resource
    Electronic Resource
    Unique fluorescence behavior of perylenetetracarboxydiimides in polyimide systems (1998)
    Bognor Regis [u.a.] : Wiley-Blackwell
    Journal of Polymer Science Part B: Polymer Physics 36 (1998), S. 827-840 
    Details
    ISSN: 0887-6266
    Keywords: polyimides ; imidization ; perylenetetracarboxydiimide ; electron transfer ; fluorescence quenching ; polyimide blends ; miscibility ; Physics ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Physics
    Notes: Perylenetetracarboxydiimide (PEDI) molecularly dispersed in polyamic acid (PAA) and polyimide (PI) films has unique fluorescence properties. An originally strong fluorescence of PEDI is efficiently quenched in the PAA films. The systematic variation of the chain structure of the PAA matrices revealed that the aromatic amide groups in the PAA chains function as a quencher. When a PAA derived from 3,4,3′4′-biphenyltetracarboxylic dianhydride (BPDA) and p-phenylenediamine (PDA), BPDA/PDA, was used as a matrix polymer, the fluorescence of the dye dispersed in the film increased abruptly as imidization of the matrix proceeds. But annealing at temperatures higher than 320°C in the step-heating process caused a gradual decrease in the fluorescence intensity. The decreased intensity results from the dye-PDA units interactions intensified by the denser molecular packing of the matrix polymer chains. PEDI shows significant dependence of the fluorescence intensity on the chain structure of the PI matrices. In the various PI films containing a fixed diamine component, the dye fluorescence intensity reduces linearly with an increase in the intramolecular charge transfer ability of the PI matrices. From the result, we propose a fluorescence quenching mechanism through multistep electron transfer processes. The BPDA/PDA polyimide matrix leads to a strong PEDI fluorescence whereas the pyromellitic dianhydride (PMDA)-based PI matrices do not. For the blends composed of these PIs, the fluorescence of PEDI bound into the main chains provides a valuable indicator of the miscibility on the molecular level. © 1998 John Wiley & Sons, Inc. J Polym Sci B: Polym Phys 36: 827-840, 1998
    Additional Material: 16 Ill.
    Type of Medium: Electronic Resource
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  • 10
    Electronic Resource
    Electronic Resource
    Self-organized microstructure growth (1995)
    Weinheim : Wiley-Blackwell
    Chemical Vapor Deposition 1 (1995), S. 81-88 
    Details
    ISSN: 0948-1907
    Keywords: Quantum dots ; Quantum wires ; Self-organization ; MOVPE ; Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: We present evidence of the new phenomenon of the direct growth of microstructures by metal-organic vapor-phase epitaxy (MOVPE) on high-index GaAs substrates. One- and zero-dimensional self-faceting by step bunching on GaAs (n11)A substrates produces quantum-wire- and dot-like arrays on GaAs(311)A and GaAs (211)A substrates. The lateral periodicity of self-faceting is controlled by the layer thickness and growth temperature and is directly correlated with the red shift of the luminescence of GaAs/AlGaAs heterostructures. On GaAs (n11)B substrates, well-ordered quantum-dot arrays are formed in a new self-organizing growth mode found in the MOVPE of a sequence of AlGaAs and strained InGaAs films. The InGaAs film spontaneously interacts with AlGaAs buffer layers to form ordered arrays of disk-shaped InGaAs quantum dots buried within AlGaAs microcrystals due to lateral mass transport. The size and distance of the disks can be controlled independently by the In composition and the InGaAs layer thickness. Similar structures are also formed on InP (311)B substrates in the GaInAs/AlInAs and GaInAs/InP material systems. Lateral confinement of carriers in the disks is confirmed by the photoluminescence linewidth which, at room temperature, is as narrow as 13 meV due to reduced thermal broadening.
    Additional Material: 12 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/cvde.19950010305
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