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  • Wiley-Blackwell  (3)
  • 1
    Electronic Resource
    Electronic Resource
    Peptide mapping of polypeptides separated by two-dimensional electrophoresis: Protease digestion directly on the two-dimensional gel followed by electrophoresis in reverse direction (1988)
    Weinheim : Wiley-Blackwell
    Electrophoresis 9 (1988), S. 339-343 
    Details
    ISSN: 0173-0835
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: A method is described which allows to reveal simultaneously the proteolytic patterns of numerous polypeptides separated by two-dimensional electrophoresis. After two-dimensional electrophoresis, the gels were dipped successively in buffers for preequilibration, protease digestion, and reequilibration. They were then returned to the electrophoresis tank, and electrophoresis was continued for a short time. After silver staining, digestion products appeared, lined up behind the original polypeptide spots. The method allows proteolytic patterns of numerous polypeptides to be viasualized simply and quickly. Among proteins of wheat leaves, 31 groups of related polypeptides were found according to the similarity of their proteolytic patterns.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/elps.1150090710
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  • 2
    Electronic Resource
    Electronic Resource
    Subcellular localization of some wheat polypeptides revealed by two-dimensional electrophoresis (1986)
    Weinheim : Wiley-Blackwell
    Electrophoresis 7 (1986), S. 476-479 
    Details
    ISSN: 0173-0835
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Cell fractionation experiments were undertaken in order to assign some of the spots revealed by two-dimensional gel electrophoresis of seven-day-old wheat etiolated seedlings to plastids or mitochondria. Among the 766 spots of these two-dimensional gels, 251 have been identified: 139 in etioplastic stroma, 139 in chloroplastic stroma, 59 in mitochondria and 23 in chloroplastic membranes. Among them, 83 are common to etioplastic and chloroplastic stroma fractions but only 11 spots are common to mitochondria and plastids sub-fractions.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/elps.1150071009
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  • 3
    Electronic Resource
    Electronic Resource
    Extraction of plant proteins for two-dimensional electrophoresis (1988)
    Weinheim : Wiley-Blackwell
    Electrophoresis 9 (1988), S. 712-718 
    Details
    ISSN: 0173-0835
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Three different extraction procedures for two-dimensional electrophoresis of plant proteins are compared: (i) extraction of soluble proteins with a nondenaturing Trisbuffer, (ii) denaturing extraction in presence of sodium dodecyl sulfate at elevated temperature allowing the solubilization of membrane proteins in addition to a recovery of soluble proteins, and (iii) a trichloroacetic acid-acetone procedure allowing the direct precipitation of total proteins.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/elps.1150091106
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