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  • 1
    Electronic Resource
    Electronic Resource
    Utilization of hydrophobic interaction, for the formation of an enzyme reactor bed (1975)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 17 (1975), S. 613-616 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Additional Material: 1 Tab.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260170413
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  • 2
    Electronic Resource
    Electronic Resource
    Reversible immobilization of enzymes to hydrophobic agarose gels (1976)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 18 (1976), S. 433-438 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260180313
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  • 3
    Electronic Resource
    Electronic Resource
    Immobilization of enzymes based on hydrophobic interaction. I. Preparation and properties of a β-amylase adsorbate (1976)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 18 (1976), S. 1573-1588 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Sweet potato β-amylase (α-1,4 glucan maltohydrolase, EC 3.2.1.2) was immobilized through adsorption onto an agarose gel to which nonpolar side chains had been introduced via ether bridges.The adsorbent showed evidence of saturation at an enzyme content of 35 mg per milliliter of packed gel. The adsorption was rapid and yielded a product whose operational stability depended on the initial content of β-amylase. Activity leakage was low. The relative activity of immobilized enzyme was inversely related to the amount of enzyme adsorbed to a given gel volume, having a maximal value of around 50% at low enzyme contents.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260181107
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  • 4
    Electronic Resource
    Electronic Resource
    Immobilization of enzymes based on hydrophobic interaction. III. Adsorbent substituent density and its impact on the immobilization of β-amylase (1976)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 18 (1976), S. 1605-1614 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Hexyl-groups have been introduced into crosslinked Sepharose 6B, yielding gels with degrees of substitution which range from 0.02 to 0.70 mol hexyl-side chain per mole galactose residue.The gels were exposed to β-amylase in solution, and the resulting adsorbates indicated a monotonic increase in adsorption capacity with an increasing hexyl-content. Adsorbate activity, by contrast, displayed a maximum for a carrier gel with a hexyl-galactose ratio of 0.51. Adsorbates based on gels with different hexyl-content were used in column reactors for continuous maltose production from a soluble starch substrate.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260181109
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  • 5
    Electronic Resource
    Electronic Resource
    Immobilization of enzymes based on hydrophobic interaction. II. Preparation and properties of an amyloglucosidase adsorbate (1976)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 18 (1976), S. 1589-1604 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Amyloglucosidase from Aspergillus niger (α-1,4 and 1,6 glucan glucohydrolase, EC 3.2.1.3) was immobilized through adsorption onto a hexyl-Sepharose, containing 0.51 mol hexyl-group per mole of galactose.The adsorption limit of the carrier with respect to this enzyme was about 17 mg per gram wet conjugate. The retention of activity upon immobilization was high, varying from essentially full activity at low enzyme content down to 68% at the adsorption limit.The immobilized preparation, as well as the soluble enzyme, showed apparent zero order kinetics within 60% of the substrate's conversion limit. Product inhibition of the soluble enzyme showed a k1 of 5 · 10-2M.In the presence of 3M NaCl, adsorbates were formed more rapidly and with a higher yield of immobilized protein, but with lower specific activity. Conjugates resulting from adsorption of amyloglucosidase in identical concentrations, but at different salt contents, showed comparable activities and operational stabilities. Continuous operation for three months reduced conjugate activity to 40%.The thermal stability of the adsorbate was inferior to that of the soluble enzyme, but was noticeably enhanced in the presence of substrate.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260181108
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  • 6
    Electronic Resource
    Electronic Resource
    Species differences in the chirality of the carbonyl reduction of [14C] fenofibrate in laboratory animals and humans (1989)
    New York, NY [u.a.] : Wiley-Blackwell
    Chirality 1 (1989), S. 197-201 
    Details
    ISSN: 0899-0042
    Keywords: fenofibrate ; chiral reduction ; species differences ; prochiral to chiral transformation ; Chemistry ; Organic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The prochiral carbonyl group of fenofibrate (isopropyl 2-[4-(4-chlorobenzoyl)phenoxy]-2-methyl propionate) is reduced during its metabolism giving rise to a chiral secondary alcohol, “reduced fenofibric acid.” Chiral and diastereomeric HPLC methods have been developed for the determination of its enantiomeric composition and these have been applied to the measurement of the “reduced fenofibric acid” enantiomers in urine of rats, guinea pigs, dogs, and human volunteers given [14C]fenofibrate. In the three animal species, the reduction is markedly enantioselective for the (-)-isomer, the enantiomeric ratios (-/ +) being 95:5. This was not due to differences in the excretion of the enantiomers, since when racemic “reduced fenofibric acid” was given to rats it was recovered in the urine with the same enantiomeric composition as the dose form. In humans the ratio was 52:48 showing the lack of stereoselectivity of reduction in this species.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/chir.530010304
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  • 7
    Electronic Resource
    Electronic Resource
    Joining hands (1989)
    New York, NY [u.a.] : Wiley-Blackwell
    Chirality 1 (1989), S. 1-1 
    Details
    ISSN: 0899-0042
    Keywords: Chemistry ; Organic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/chir.530010102
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  • 8
    Electronic Resource
    Electronic Resource
    Editors' note (1998)
    New York, NY [u.a.] : Wiley-Blackwell
    Chirality 10 (1998), S. iii 
    Details
    ISSN: 0899-0042
    Keywords: Chemistry ; Organic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: No abstract.
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
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  • 9
    Electronic Resource
    Electronic Resource
    Polyesters of hydroxybenzoic acids (1959)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Applied Polymer Science 2 (1959), S. 198-202 
    Details
    ISSN: 0021-8995
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Polymers and copolymers were prepared from the acetoxy derivatives of m- and p-hydroxybenzoic acids. Molecular weights of over 20,000 were obtained for poly(m-hydroxybenzoic acid) and copolymers containing up to 60% para isomer. These polyesters were crystalline, and the degree of crystallinity increased as the para isomer content was increased. Blocks of the high-melting, insoluble poly(p-hydroxybenzoic acid) tend to form in copolymers containing a high proportion of the para isomer because of the difference in reactivity of the two isomers. Copolymers containing at least 55% of the meta isomer are soluble in several classes of solvents. The copolymers do not have sharply defined melting points, but have softening temperatures ranging from 150°C. to over 300°C. Tensile strengths of approximately 10,000 psi and elongations of about 6% to 40% were obtained. The polymers were relatively stable to oxidative degradation, but not to hydrolysis.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/app.1959.070020512
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  • 10
    Electronic Resource
    Electronic Resource
    Elastomers based on polycyclic bisphenol polycarbonatesPresented in part at the Polycarbonate Symposium, Southeastern Regional Meeting of the American Chemical Society, October 15-17, 1964, Charleston, West Virginia. (1965)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Applied Polymer Science 9 (1965), S. 3451-3463 
    Details
    ISSN: 0021-8995
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Polycarbonate elastomers were prepared by the addition of phosgene to a pyridine solution of poly(tetramethylene ether) glycol and bisphenols containing norbornanetype groups. These elastomers do not contain the classical “tie-down” points - crosslinks, hydrogen bonding, or crystallinity - which, heretofore, have been considered necessary for good elastomer properties. This work introduces a new concept in elastomer chemistry: bulky, three-dimensional groups are effective tie-down points. Most of the elastomers were prepared with 4,4'-(2-norbornylidene)bis(2,6-dichlorophenol). For comparison, several elastomers with bisphenol-A were also prepared. A statistically designed experiment was carried out to determine the effect of composition variables on the properties of these elastomers. The properties were determined on films cast from methylene chloride and, in many cases, on wet-spun fibers. These elastomers have elongations of 400-600% and instantaneous elastic recoveries up to 100%.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/app.1965.070091022
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