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  • 1
    Electronic Resource
    Electronic Resource
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 15 (1992), S. 319-328 
    ISSN: 0935-6304
    Keywords: Organochlorine pesticides ; Fused silica open tubular column ; Dual-column, dual-detector gas chromatographic system ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Forty five organochlorine pesticides (currently listed in EPA Method 8081A) have been chromatographed on DB-5/DB-1701 and SPB-5/SPB-1701 column pairs which were in each case connected to an inlet splitter and separate electron capture detectors. Thirteen additional compounds were evaluated for their suitability as internal standards or surrogate compounds for incorporation into Method 8081A. Method reproducibility and linearity are discussed, and results are presented for extracts of six real samples spiked with fifteen organochlorine pesticides and analyzed using the dual-column, dual-detector arrangement.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 15 (1992), S. 160-164 
    ISSN: 0935-6304
    Keywords: Halogenated diphenyl ethers ; Nitrated diphenyl ethers ; Fused silica open tubular column ; Dual-column dual-detector GC ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Nineteen halogenated and/or nitrated diphenyl ethers (currently listed in EPA Method 8111) have been separated on a DB-5/ DB-1701 column pair connected to an inlet splitter and separate electron capture detectors. Retention times are included for 10 additional compounds evaluated for their suitability as internal standards or surrogate compounds for incorporation into Method 8111. Method reproducibility and linearity are discussed, and results are presented for extracts of two real samples spiked with the 19 diphenyl ethers and analyzed using the dual-column dual-detector arrangement.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 14 (1991), S. 601-607 
    ISSN: 0935-6304
    Keywords: Nitroaromatic compounds ; Fused silica open tubular column ; Dual-column/dual-detector GC system ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Thirty five nitroaromatic compounds (currently listed in EPA Method 8091) and 1,4-naphthoquinone have been separated on DB-5-DB-1701 and SPB-5-SPB-1701 column pairs, which were in each case connected to an inlet splitter and separate electron capture detectors. Retention times are included for 21 additional compounds evaluated for their suitability as internal standards and/or surrogate compounds for incorporation into Method 8091. Method reproducibility and linearity are discussed, and results are presented for extracts of two real samples spiked with the 35 nitroaromatic compounds and 1,4-naphthoquinone and analyzed using the dual-column-dual-detector arrangement.
    Additional Material: 4 Ill.
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  • 4
    Electronic Resource
    Electronic Resource
    New York, NY : Wiley-Blackwell
    Helvetica Chimica Acta 36 (1953), S. 597-605 
    ISSN: 0018-019X
    Keywords: Chemistry ; Organic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Reprenant l'etude de la constitution de l'acide chondroïtine-sulfurique, nous avons pu établir: 1° Que la liaison chondrosaminique se trouve bien en C1-C4, conformément aux conclusions de Wolfrom.2° Que la liaison glucuronique se trouve en C1-C6 et non pas C1-C3.3° Que le groupe sulfate est situé en C3 ou C4 de la chondrosamine.
    Additional Material: 2 Ill.
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  • 5
    ISSN: 0749-503X
    Keywords: Schizosaccharomyces pombe ; cell cycle ; Cdc2 kinase ; GST ; Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: Characterization of cdk (cyclin dependent kinases) substrates and studies of their regulation require purified enzymatic complexes of cdc2-related catalytic and cyclin regulatory subunits. We produced human Cdc2 kinase in the fission yeast Schizosaccharomyces pombe as a fusion protein with glutathione S-transferase (GST). The GST-human Cdc2p fusion protein was active in vivo since it rescued a temperature-sensitive allele of cdc2. The fusion protein was purified using a one-step chromatography procedure with glutathione-Sepharose and exhibited a catalytic activity in vitro. Yeast cyclin B and suc1 were found in association with GST-Cdc2. A 17-fold stimulation of GST-Cdc2 kinase activity was obtained by incubation of recombinant human cyclin A with the S. pombe cellular extract prior to affinity purification. This indicates that cyclin concentration is limiting in this overexpression system. These findings describe a fast and easy production of active recombinant human Cdc2 kinase in yeast that can be used for biochemical studies.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
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