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  • Wiley-Blackwell  (64)
  • 1975-1979  (64)
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Year
  • 1
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 17 (1975), S. 695-701 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Six enzymes associated with the activities of the nucleus (thymidine kinase), mitochondria (succinate dehydrogenase), lysosomes (acid phosphatase), peroxisomes (catalase), (cytosol lactate dehydrogenase), and the intermembranal mitochondrial space (alkaline DNase) were assayed at 2 hr intervals over the division cycle of repetitively resynchronized HeLa cells. The results indicated a high degree of reproductibility for cells synchronized by the method of perpetual resynchronization and may be of direct use to those interested in subcellular organellogenesis.
    Additional Material: 7 Ill.
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  • 2
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 17 (1975), S. 703-712 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Unbalanced growth has been studied in HeLa cell cultures maintained in perpetual division synchrony by periodic inhibition of DNA synthesis. Observation of eight independent cellular parameters indicates that once synchrony has been established the degree of unbalanced growth in later cycles is not as great as is observed with the conventional “double-block” technique. This significant diminution of unbalanced growth appears to be related to the significantly shorter periods of inhibition of DNA synthesis used in the resynchronization process.
    Additional Material: 5 Ill.
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  • 3
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 21 (1979), S. 433-442 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A variety of diploid human fibroblast lines have been successfully grown to high densities (〉106 cell/ml) on recently developed microcarriers. Interferon induction using poly I·poly C and a superinduction procedure resulted in yields greater than 10,000 units/ml with one cell line. A direct comparison of microcarrier cultures to roller bottle cultures showed equivalent interferon yields on a per cell basis and some apparent differences relating to optimum inducer concentrations and kinetics of interferon accumulation.
    Additional Material: 2 Ill.
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  • 4
    ISSN: 0020-7608
    Keywords: Computational Chemistry and Molecular Modeling ; Atomic, Molecular and Optical Physics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The potential energy curves of the bending mode of PH2 in the ground 2B1 and the excited 2A1 states have been calculated using ab initio SCF-MO wave-functions. The radical is found, in agreement with experiment, to be bent in the two states. The calculated results clarify the interpretations of the anomalous behaviour of observed vibrational intervals, and offer an explanation of the ‘quasilinearity’; of the radical in the 2A1 state.
    Additional Material: 1 Ill.
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  • 5
    Electronic Resource
    Electronic Resource
    Chichester [u.a.] : Wiley-Blackwell
    International Journal for Numerical Methods in Engineering 13 (1978), S. 17-33 
    ISSN: 0029-5981
    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mathematics , Technology
    Notes: The fluid forces acting on a uniform cylinder with an infinitely long axis, heaving in the free surface or an infinite ideal fluid, are described in terms of its ‘added mass’ and ‘damping coefficient’. The techniques of multipole expansion and multiparameter conformal transformation are adopted for such calculations and applied to shapes which cannot be adequately represented by the conventional, and more rudimentary, ‘Lewis form fit’. The shapes referred to are both relevant to ship bows, one being a ‘fine section’ and the other a ‘bulbous section’. The parameters which influence the accuracy of the solution are examined. Results for these two sections are computed and compared with results based on (a) the ‘Lewis form approximation’ and (b) the ‘Frank's close fit method’ which employs a singularity representation.
    Additional Material: 8 Ill.
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  • 6
    Electronic Resource
    Electronic Resource
    Chichester [u.a.] : Wiley-Blackwell
    International Journal for Numerical Methods in Engineering 14 (1979), S. 665-679 
    ISSN: 0029-5981
    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mathematics , Technology
    Notes: A study of the application of the Finite Element Method to compressible potential flows, typified by the airfoil problem, is undertaken. Some novel approaches, believed to simplify solution techniques, are presented.The solutions use two pseudo-variational integrals, appropriate to subsonic flows, and possessing a physical iterative basis. With constant-derivatives triangular elements formulated for cylindrical co-ordinates, accurate solutions are easily obtained for the flow over a circular cylinder. For arbitrary airfoils a simple mapping is used to transform them into near circles. An appropriate mesh is then constructed in the mapped plane. The paper then presents two solution approaches by which this non-linear problem is solved in both the near circle plane and the airfoil plane.
    Additional Material: 9 Ill.
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  • 7
    Electronic Resource
    Electronic Resource
    Weinheim : Wiley-Blackwell
    Materialwissenschaft und Werkstofftechnik 6 (1975), S. 361-367 
    ISSN: 0933-5137
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Description / Table of Contents: The Use of Titanium Castings to Produce a Complex Shaped Intermediate Casing of RB 199.This paper describes the present position on a highly stressed casing and its functions in the RB 199-34 R engine. When the parts of the casing are made from solid forged titanium billets, up to 93% of the material used is machined away. For this reason, the most complicated parts of the casing were procured as castings and welded together by TIG welding. The paper describes the present quality standard of the titanium castings. The tests on the materials and their mechanical properties show that the castings are inferior to the forgings only with respect to their HCF behaviour and their elongation at rupture. Macroscopic flaws are not entirely inevitable but can be detected by X-ray tests. The future prospects for titanium castings are considered favourable.
    Notes: Der Bericht beschreibt die Lage eines hochbeanspruchten Gehäuses und dessen Funktionen im Triebwerk RB 199-34 R. Bei der Herstellung der Einzelteile des Gehäuses aus massiv geschmiedeten Titanblöcken wird bis zu 93% des eingesetzten Materials zerspant. Die kompliziertesten Partien des Gehäuses wurden deshalb als Gußteil bezogen und mittels WIG-Schweißung verbunden. Der Bericht beschreibt den heutigen Qualitätsstand der Titangußteile. Die Ergebnisse der Werkstoff- und Festigkeitsuntersuchungen weisen nur im HCF-Verhalten und der Bruchdehnung Nachteile des Gusses gegenüber dem geschmiedeten Material aus. Makroskopische Fehler sind nicht vollständing vermeidbar, aber durch Röntgenprüfung nachzuweisen. Die künftigen Aussichten des Titangusses werden positiv beurteilt.
    Additional Material: 14 Ill.
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  • 8
    Electronic Resource
    Electronic Resource
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 2 (1979), S. 221-224 
    ISSN: 0935-6304
    Keywords: Gas chromatography ; Capillary ; Vapor-phase sampling ; Sample preparation, extraction by carbon dioxide under high pressure, Freon 12 ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Two new sample preparation procedures that produce solvent-free extracts especially suitable for use with high resolution GC are described. One is based on Soxhlet extraction in a pressurized container that permits the use of liquid carbon dioxide as the extractant; the other utilizes refluxing difluorodichloro-methane (Freon 12) to isolate entrained volatiles from gas streams.
    Additional Material: 6 Ill.
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  • 9
    Electronic Resource
    Electronic Resource
    New York, NY : Wiley-Blackwell
    Helvetica Chimica Acta 62 (1979), S. 593-608 
    ISSN: 0018-019X
    Keywords: Chemistry ; Organic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The electronic properties of a variety of methylated isoalloxazine derivatives have been investigated with absorption and fluorescece techniques (Table 1). The spectral effects of methyl substitution at different positions in the isoalloxazine ring are interpreted in terms of different bond orders between the C-atoms of the methyl groups and of the aromatic ring. Methyl groups at C(6) or C(9) induce quenching of the fluorescence and shortening of the lifetime whereas a methyl group at C(7) has an opposite effect. The quenching effect might be due to strain in the molecular framework caused by the methyl groups.The lowest energy levels of the isoalloxazine cations are shifted to higher energy as compared to the neutral isoalloxazines. Apart from these shifts the trends in spectral behaviour upon methylation are similar to those observed for the neutral isoalloxazine (cf. Table 2). The fluorescence of the isoalloxazine cations is strongly quenched in auqeous solution and their lifetime much shortened. These observations indicated that there is substantial interaction between the charged fluorophore and the water dipoles and that this interaction produces efficient radiationless deactivation of the first excited singlet state.
    Additional Material: 4 Ill.
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  • 10
    ISSN: 0006-3525
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Conformational transitions induced by pH changes in random copolymers of leucine and glutamic acid have been studied. Significant differences were observed in the potentiometric titration curves of copolymers with small (up to 4%) and large leucine contents. The helical stability of copolymers with small leucine content, determined from titration curves by the Zimm and Rice method, decreases slightly with an increase in the leucine content, whereas the helical stability of copolymers with large leucine content increases sharply with an increase of the leucine content. It is shown that copolymers with large leucine content aggregate in the region of transition into the helical state, but the increase of their helical state stability is not connected with intermolecular aggregation, as it was also observed for a nonaggregating fraction isolated from one of the copolymers by gel chromatography. A conclusion is made that the helix-coil equilibrium constant s for leucine does not itself exceed the s constant for uncharged polyglutamic acid. The stabilization of the helical state in copolymers with large leucine content is due to intramolecular aggregation of helices in these copolymers. The analysis of the leucine residue distribution between helical and nonhelical regions in globular proteins also gives no real arguments to ascribe special helix-forming properties to leucine.
    Additional Material: 5 Ill.
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