Publication Date:
2011-11-04
Description:
Compared to conventional protein microarrays, the aptamer microarray is a relatively new and competitive method for analytical applications. The three-dimensional folded aptamers show excellent binding specificity and affinity, and thus, can be used as an alternative to antibodies. Immobilization and binding effects of aptamers under different combinations of surfaces and spacers for aptamer-microarray applications are investigated. In addition, the effects of spacers integrated at the terminal position of aptamers on made in-house and commercially available microarray supports are explored. Microarray surfaces and spacers may affect the binding efficiency and quality between aptamers and their corresponding targets. Various surfaces modified with amino, epoxy, and aldehyde functional groups with short, middle, and long spacers of linear and branched type were investigated. Spacers fused to the terminal end of aptamers were tested and compared with spacers fused on microarray supports.
Print ISSN:
0930-7516
Electronic ISSN:
1521-4125
Topics:
Chemistry and Pharmacology
,
Process Engineering, Biotechnology, Nutrition Technology
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