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  • 1
    Publikationsdatum: 2019-05-01
    Print ISSN: 0047-2425
    Digitale ISSN: 1537-2537
    Thema: Energietechnik , Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Publiziert von Wiley
    Standort Signatur Erwartet Verfügbarkeit
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  • 2
    Publikationsdatum: 2019
    Beschreibung: Abstract (1‐x)Sr0.7Pb0.15Bi0.1TiO3‐xBi4Ti3O12 ((1‐x)SPBT‐xBIT, x = 0‐0.125) bulk ceramics were developed and calcined via the solid‐state method, aimed at the application of pulsed power capacitors. The phase structures, temperature stability, hysteresis loop, and discharge properties were systematically investigated. Considering both the temperature stability and dielectric properties, 0.925SPBT‐0.075BIT bulk ceramics with a capacitance variation satisfying the X7R specification were developed for pulsed power capacitors. The energy storage density was 0.252 J/cm3, and the ceramics showed high temperature stability at 80 kV/cm. The discharge current waveforms of the 0.925SPBT‐0.075BIT ceramics were recorded. A high discharge power density of approximately 1.01 × 108 W/kg with an 8 Ω load resistor and short discharge period of 84 ns were achieved at 50 kV/cm. The good temperature stability properties and high power density show that the 0.925SPBT‐0.075BIT ceramics are well suited for pulsed power capacitors with a wide temperature range.
    Print ISSN: 0002-7820
    Digitale ISSN: 1551-2916
    Thema: Maschinenbau , Physik
    Publiziert von Wiley
    Standort Signatur Erwartet Verfügbarkeit
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  • 3
    Publikationsdatum: 2012-06-19
    Beschreibung: The quality and safety of human embryonic stem cells (hESCs) in clinical application depend on gene stability. Two Chinese hESC lines, Zh1 and Zh21, were incubated over a long period. We observed and compared the gene stability in the passage numbers 20, 17 for Zh1 cell line and passage numbers 27, 60, 68 for Zh21 cell line. Single nucleotide polymorphisis analysis indicated that hESCs in early passages had relative gene stability; and with the increase in passage number, gene instability became strong. We also found that there were copy number variations (CNVs) in both Zh21 and Zh1. We analyzed the CNVs of Chinese Han Beijing man (CHB) (normal Chinese people and found that the all CNV forms were the loss in Zh21, Zh1 and CHB. We also analyzed and compared the related pathways of the mutant genes. We propose three steps to ensure hESC safety. Firstly, besides the conventional methods such as pluripotent genes, chromosome G-banding and teratoma, high-resolution DNA chip analysis should also be adopted; secondly, chromosomal properties are monitored every 10 passages in less than passage 50 and every 5 passages in more than passage 50; thirdly, the related pathways of mutant genes should be observed because only the mutant genes with variations of their related pathways may affected cell functions. J. Cell. Biochem. © 2012 Wiley Periodicals, Inc.
    Digitale ISSN: 0091-7419
    Thema: Biologie , Chemie und Pharmazie , Medizin
    Publiziert von Wiley
    Standort Signatur Erwartet Verfügbarkeit
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  • 4
    Publikationsdatum: 2019
    Beschreibung: Abstract As the field of flash sintering expands, more diverse flash processes are emerging that exhibit complex mechanisms and kinetics. Reactive flash sintering studies have been performed using precursor oxides and have yet to explore redox reactions. We show that Mn2O3 transforms into Mn2O3 during stage III of flash sintering via a moving reaction front, propagating from an electrode if sufficient energy is supplied. The power density and sample temperature increase as the transformation progresses due to the lower resistivity of Mn2O3 vs. Mn2O3, a secondary thermal runaway effect, further confirming the presence of a transformation front. Additionally, in many studies contact resistance is accounted for, but not utilized. The energy for the transformation may either be supplied by contact resistance‐induced Joule heating or the furnace. Room temperature impedance measurements demonstrate that Pt electrodes provide substantial contact resistance while Ag electrodes do not. The impedance study demonstrates it is critical to select the appropriate electrode material whether the goal is to maximize or minimize contact resistance. Contact resistance may be used to create a hot spot and propagate a transformation front in any endothermic reduction reaction that occurs below 950°C in electronic conductors. This article is protected by copyright. All rights reserved.
    Print ISSN: 0002-7820
    Digitale ISSN: 1551-2916
    Thema: Maschinenbau , Physik
    Publiziert von Wiley
    Standort Signatur Erwartet Verfügbarkeit
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  • 5
    Publikationsdatum: 2014-02-13
    Beschreibung: Two Ln III complexes based on a nitronyl nitroxide radical were synthesized and characterized structurally and magnetically: [ Ln (hfac) 3 [NITPh m -Cl] 2 ][0.5CH 3 (CH 2 ) 5 CH 3 ] [ Ln = Er ( 1 ), Nd ( 2 )] [hfac = hexafluoroacetylacetonate, NITPh m -Cl = 2-(3′-chlorophenyl)-4, 4,5, 5-tetramethylimidazoline-1-oxyl-3-oxide]. X-ray crystal structure analysis revealed that the two complexes have similar mononuclear tri-spin structures, in which the metal ions are octacoordinated by two radicals and three hfac molecules to form a slightly distorted dodecahedral arrangement. Each tri-spin unit connects through intermolecular hydrogen bonds, resulting in the formation of one-dimensional wave-like chain. Both of the complexes form a one-dimensional structure by intermolecular hydrogen bonds. Variable temperature magnetic susceptibility measurements studies revealed that there are antiferromagnetic interactions between the paramagnetic ions ( Ln III and radical).
    Print ISSN: 0044-2313
    Digitale ISSN: 1521-3749
    Thema: Chemie und Pharmazie
    Publiziert von Wiley
    Standort Signatur Erwartet Verfügbarkeit
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  • 6
    Publikationsdatum: 2011-12-17
    Beschreibung: To investigate the impact of extreme drought and the Three Gorges Dam on organic carbon (OC) exported by the Changjiang (Yangtze) River, suspended particulate matter samples collected in 2003 (normal year) and 2006 (drought year) from the middle and lower reaches of the mainstream of the river were analyzed for elemental carbon, stable carbon isotope, and lignin-phenol yields. In comparison with suspended particulate matter samples collected in 2003, the 2006 samples showed elevated organic carbon content [OC%: (1.91 ± 0.43)% and (0.95 ± 0.07)% respectively in 2006 and 2003], higher autochthonous OC [Chl-a/OC: (20.68 ± 9.03)mg/g OC and (7.33 ± 2.97)mg/g OC in 2006 and 2003], decreased lignin yields [Λ8: (0.90 ± 0.41)mg/100 mg OC and (1.91 ± 0.33)mg/100 mg OC in 2006 and 2003], and higher degradation state [(Ad/Al)v: 0.44 ± 0.21 and 0.27 ± 0.028 in 2006 and 2003]. These differences may be explained by changes in grain composition and concentration of the suspended particulate matter, which were caused by the lower water discharge and sediment load in 2006. Extreme drought and impoundment of water by the Three Gorges Dam not only affected the signatures and fluxes of terrestrial OC, but hid the seasonal signal of riverine OC. The impact of the Three Gorges Dam was more significant in the middle reach than in the lower reach. Lakes and tributaries in the lower Changjiang River supplied great amounts of terrestrial OC to the mainstream. In the drought year, the supplement of water provided by the lower lakes and tributaries was more significant. The absolute magnitude of reduction of terrestrial OC in the Changjiang River during drought and Three Gorges Dam influence (2006) would have severely affected the local carbon cycle and would have influenced the global carbon cycle to some extent.
    Print ISSN: 0148-0227
    Thema: Geologie und Paläontologie , Physik
    Publiziert von Wiley im Namen von American Geophysical Union (AGU).
    Standort Signatur Erwartet Verfügbarkeit
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  • 7
    Publikationsdatum: 2012-11-12
    Beschreibung: Understanding the dynamics and underlying mechanism of carbon exchange between terrestrial ecosystems and the atmosphere is one of the key issues in global change research. In this study, we quantified the carbon fluxes in different terrestrial ecosystems in China, and analyzed their spatial variation and environmental drivers based on the long-term observation data of ChinaFLUX sites and the published data from other flux sites in China. The results indicate that gross ecosystem productivity (GEP), ecosystem respiration (ER) and net ecosystem productivity (NEP) of terrestrial ecosystems in China showed a significantly latitudinal pattern, declining linearly with the increase of latitude. However, GEP, ER and NEP did not present a clear longitudinal pattern. The carbon sink functional areas of terrestrial ecosystems in China were mainly located in the subtropical and temperate forests, coastal wetlands in eastern China, the temperate meadow steppe in the northeast China, and the alpine meadow in eastern edge of Qinghai-Tibetan Plateau. The forest ecosystems had stronger carbon sink than grassland ecosystems. The spatial patterns of GEP and ER in China were mainly determined by mean annual precipitation (MAP) and mean annual temperature (MAT), while the spatial variation in NEP was largely explained by MAT. The combined effects of MAT and MAP explained 79%, 62% and 66% of the spatial variations in GEP, ER and NEP, respectively. The GEP, ER and NEP in different ecosystems in China exhibited ‘positive coupling correlation’ in their spatial patterns. Both ER and NEP were significantly correlated with GEP, with 68% of the per-unit GEP contributed to ER and 29% to NEP. MAT and MAP affected the spatial patterns of ER and NEP mainly by their direct effects on the spatial pattern of GEP. © 2012 Blackwell Publishing Ltd
    Print ISSN: 1354-1013
    Digitale ISSN: 1365-2486
    Thema: Biologie , Energietechnik , Geographie
    Publiziert von Wiley
    Standort Signatur Erwartet Verfügbarkeit
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  • 8
    Publikationsdatum: 2017-05-18
    Beschreibung: Epigenetic modifications such as histone modifications and cytosine hydroxymethylation are linked to tumorigenesis. Loss of 5-hydroxymethylcytosine (5hmC) by ten-eleven translocation 1 (TET1) down-regulation facilitates tumor initiation and development. However, the mechanisms by which loss of TET1 knockdown promotes malignancy development remains unclear. Here, we report that TET1 knockdown induced epithelial-mesenchymal transition (EMT) and increased cancer cell growth, migration and invasion in DLD1 cells. Loss of TET1 increased EZH2 expression and reduced UTX-1 expression, thus increasing histone H3K27 tri-methylation causing repression of the target gene E-cadherin. Ectopic expression of the H3K27 demethylase UTX-1 or EZH2 depletion both impeded EZH2 binding caused a loss of H3K27 methylation at epithelial gene E-cadherin promoter, thereby suppressing EMT and tumor invasion in shTET1 cells. Conversely, UTX-1 depletion and ectopic expression of EZH2 enhanced EMT and tumor metastasis in DLD1 cells. These findings provide insight into the regulation of TET1 and E-cadherin and identify EZH2 as a critical mediator of E-cadherin repression and tumor progression.This article is protected by copyright. All rights reserved
    Digitale ISSN: 1097-4652
    Thema: Biologie , Medizin
    Publiziert von Wiley
    Standort Signatur Erwartet Verfügbarkeit
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  • 9
    Publikationsdatum: 2018-01-19
    Beschreibung: Alzheimer's disease, one of the most common neurodegenerative diseases, is pathologically characterized by Amyloid beta containing plaques and neurofibrillary tangles. Amyloid beta (Aβ) induces neuronal apoptosis through the intracellular Ca 2+ increase, subsequent hyperactivation of cyclin-dependent kinase 5 (Cdk5) and mitochondrial abnormality. Recently, Cdk5 was identified as an upstream regulator of mitochondrial fission during neuronal apoptosis, but the underlying mechanism remains unclear. Here, in vitro phosphorylation assays showed that Cdk5 could phosphorylate the recombinant Drp1 at Serine 579. Aβ 1-42 stimulation increased the phosphorylation level of Drp1 at Serine 579 in mouse cortical neurons. Cdk5 inhibitor roscovitine and knockdown of Cdk5 by a lentiviral vector expressing shRNA targeting Cdk5 (Lenti-Cdk5-shRNA) efficiently prevented Aβ 1-42 induced Drp1 phosphorylation in neurons. In addition, Aβ 1-42 stimulation induced markedly mitochondrial fission in neurons. Roscovitine, Lenti-Cdk5-shRNA and expression of phospho-defect mutatant GFP-Drp1-S579A in neurons attenuated Aβ 1-42 induced mitochondrial fission, whereas expression of phospho-mimetic mutant GFP-Drp1-S579D alone resulted in mitochondiral fission similar to Aβ 1-42 stimulation. Moreover, Roscovitine and Lenti-Cdk5-shRNA suppressed the cleavage of caspase-3 and protected neurons against Aβ 1-42 induced neuronal apoptosis.Thus, our data indicate that Drp1 is a direct target of Cdk5, and Cdk5-mediated phosphorylation of Drp1 at Serine 579 regulates Aβ 1-42 induced mitochondrial fission and neuronal toxicity. This article is protected by copyright. All rights reserved
    Digitale ISSN: 0091-7419
    Thema: Biologie , Chemie und Pharmazie , Medizin
    Publiziert von Wiley
    Standort Signatur Erwartet Verfügbarkeit
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  • 10
    Publikationsdatum: 2017-01-07
    Beschreibung: In our previous study, we revealed that Cyclosporin A (CsA) could inhibit miR-144 expression to regulate proliferation and invasion of human trophoblast (HT) cells through miR-144 targeting titin. This partially demonstrated the mechanism by which CsA promotes titin expression to increase the vitality of HT cells. However, the mechanism by which CsA inhibits miR-144 expression remains to be investigated. Recently, the interaction between lncRNA and miRNA has been frequently reported to play major role in several biological processes. In the present study, online tools were used to figure out that X-inactive specific transcript (XIST) could interact with miR-144. XIST and miR-144 reciprocally inhibited each other in HT cells; as exhibited by luciferase reporter gene assays, miR-144 bind to XIST by direct targeting. XIST suppressed miR-144 expression to promote titin expression. As exhibited by the Spearman's correlation analysis, in CsA treated HT cells, miR-144 was inversely correlated with titin and XIST, respectively; XIST was positively correlated with titin. Moreover, CsA could promote the proliferation and invasion of HT cells through XIST and the downstream MAPK and MMPs pathway. Taken together, these findings will shed light to the role and mechanism of CsA/XIST/miR-144/titin in regulating HT cells proliferation and invasion. XIST may serve as a potential therapeutic target in HT in the future. This article is protected by copyright. All rights reserved
    Digitale ISSN: 0091-7419
    Thema: Biologie , Chemie und Pharmazie , Medizin
    Publiziert von Wiley
    Standort Signatur Erwartet Verfügbarkeit
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