Publication Date:
2017-07-14
Description:
An adequate characterization of adsorption isotherms is mandatory for chromatographic process development. Here, the elution by characteristic point (ECP) is a suitable method with low consumption of material and time. However, the ECP method requires highly ideal behavior of the measured system. This study presents an innovative extended ECP approach to characterize a non-ideal system by the example of protein ion-exchange adsorption. A marker, here bovine Hemoglobin, is used to quantify all non-idealities, e.g. convection, dispersion or low number of theoretical plates (app. 10 theoretical plates). The system signal of the marker is used to compensate the system signal of the interesting solute, here bovine Serum Albumin. The resulting isotherm agrees with the control results of a validated static approach since the static data are within the 68% confidence interval of the ECP data.
Print ISSN:
0930-7516
Electronic ISSN:
1521-4125
Topics:
Chemistry and Pharmacology
,
Process Engineering, Biotechnology, Nutrition Technology
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