ALBERT

Description: ABSTRACT Friedreich's ataxia (FRDA) is an autosomal recessive inherited disorder involving progressive damage to the central and peripheral nervous systems and cardiomyopathy. FRDA is caused by the silencing of the FXN gene and reduced levels of the encoded protein, frataxin. Frataxin is a mitochondrial protein that functions primarily in iron-sulfur cluster synthesis. Skin disorders including hair abnormalities have previously been reported in patients with mitochondrial disorders. However, to our knowledge, ultra-structural hair alterations in FRDA were not demonstrated. The purpose of this study was to determine ultra-structural alterations in the hairs of FRDA patients as well as carriers. Hair specimen from four patients, who are in different stages of the disease, and two carriers were examined by scanning electron microscope. Thin and weak hair follicles with absence of homogeneities on the cuticular surface, local damages of the cuticular layer, cuticular fractures were detected in both carriers and patients, but these alterations were much more prominent in the hair follicles of patients. In addition, erosions on the surface of the cuticle and local deep cavities just under the cuticular level were observed only in patients. Indistinct cuticular pattern, pores on the cuticular surface, and presence of concavities on the hair follicle were also detected in patients in later stages of the disease. According to our results, progression of the disease increased the alterations on hair structure. We suggest that ultra-structural alterations observed in hair samples might be due to oxidative stress caused by deficient frataxin expression in mitochondria. Microsc. Res. Tech., 2015 . © 2015 Wiley Periodicals, Inc.

Description: Di(ethylhexyl)phthalate (DEHP), the most widely used plasticizer, was investigated to determine whether an oxidative stress process was one of the underlying mechanisms for its testicular toxicity potential. To evaluate the effects of selenium (Se), status on the toxicity of DEHP was further objective of this study, as Se is known to play a critical role in testis and in the modulation of intracellular redox equilibrium. Se deficiency was produced in 3-weeks-old Sprague–Dawley rats feeding them ≤0.05 mg Se /kg diet for 5 weeks, and Se-supplementation group was on 1 mg Se/kg diet. DEHP-treated groups received 1000 mg/kg dose by gavage during the last 10 days of the feeding period. Activities of antioxidant selenoenzymes [glutathione peroxidase 1 (GPx1), glutathione peroxidase 4 (GPx4), thioredoxin reductase (TrxR)], catalase (CAT), superoxide dismutase (SOD), and glutathione S -transferase (GST); concentrations of reduced glutathione (GSH), oxidized glutathione (GSSG), and thus the GSH/GSSG redox ratio; and thiobarbituric acid reactive substance (TBARS) levels were measured. DEHP was found to induce oxidative stress in rat testis, as evidenced by significant decrease in GSH/GSSG redox ratio (〉10-fold) and marked increase in TBARS levels, and its effects were more pronounced in Se-deficient rats with ∼18.5-fold decrease in GSH/GSSG redox ratio and a significant decrease in GPx4 activity, whereas Se supplementation was protective by providing substantial elevation of redox ratio and reducing the lipid peroxidation. These findings emphasized the critical role of Se as an effective redox regulator and the importance of Se status in protecting testicular tissue from the oxidant stressor activity of DEHP. © 2011 Wiley Periodicals, Inc. Environ Toxicol, 2011.

Description: Di(ethylhexyl)phthalate (DEHP), the most widely used plasticizer, was investigated to determine whether an oxidative stress process was one of the underlying mechanisms for its testicular toxicity potential. To evaluate the effects of selenium (Se), status on the toxicity of DEHP was further objective of this study, as Se is known to play a critical role in testis and in the modulation of intracellular redox equilibrium. Se deficiency was produced in 3-weeks-old Sprague–Dawley rats feeding them ≤0.05 mg Se /kg diet for 5 weeks, and Se-supplementation group was on 1 mg Se/kg diet. DEHP-treated groups received 1000 mg/kg dose by gavage during the last 10 days of the feeding period. Activities of antioxidant selenoenzymes [glutathione peroxidase 1 (GPx1), glutathione peroxidase 4 (GPx4), thioredoxin reductase (TrxR)], catalase (CAT), superoxide dismutase (SOD), and glutathione S -transferase (GST); concentrations of reduced glutathione (GSH), oxidized glutathione (GSSG), and thus the GSH/GSSG redox ratio; and thiobarbituric acid reactive substance (TBARS) levels were measured. DEHP was found to induce oxidative stress in rat testis, as evidenced by significant decrease in GSH/GSSG redox ratio (〉10-fold) and marked increase in TBARS levels, and its effects were more pronounced in Se-deficient rats with ∼18.5-fold decrease in GSH/GSSG redox ratio and a significant decrease in GPx4 activity, whereas Se supplementation was protective by providing substantial elevation of redox ratio and reducing the lipid peroxidation. These findings emphasized the critical role of Se as an effective redox regulator and the importance of Se status in protecting testicular tissue from the oxidant stressor activity of DEHP. © 2011 Wiley Periodicals, Inc. Environ Toxicol, 2011.

Description: Dimethyl heptalene-4,5-dicarboxylates † undergo preferentially a Michael addition reaction at C(3) with α -lithiated alkyl phenyl sulfones at temperatures below −50°, leading to corresponding cis -configured 3,4-dihydroheptalene-4,5-dicarboxylates ( cf. Table 1, Schemes 3 and 4 ). The corresponding heptalenofuran-1-one-type pseudoesters of dimethyl heptalene-4,5-dicarboxylates ( Scheme 5 ) react with [(phenylsulfonyl)methyl]lithium almost exclusively at C(1) of the furanone group ( Scheme 6 ). In contrast to this expected behavior, the uptake of 1-[phenylsulfonyl)ethyl]lithium occurs at C(5) of the heptalenofuran-1-ones as long as they carry a Me group at C(11) ( Schemes 6 and 7 ). The 1,4- as well as the 1,6-addition products eliminate, on treatment with MeONa/MeOH in THF, benzenesulfinate, thus leading to 3- and 4-alkylated dimethyl heptalene-4,5-dicarboxylates, respectively ( Schemes 8 – 13 ). The configuration of the addition reaction of the nucleophiles to the inherently chiral heptalenes is discussed in detail ( cf. Schemes 14 – 19 ) on the basis of a number of X-ray crystal-structure determinations as well as by studies of the temperature-dependence of the 1 H-NMR spectra of the addition products.

Description: We have studied Juniperus oxycedrus L. subsp. badia (H.Gay) Debeaux in the central and southern Iberian Peninsula, where the macrobioclimate ranges from Mediterranean-pluviseasonal-oceanic to Mediterranean-pluviseasonal-continental, and the thermotype from the thermo- to the supramediterranean. The relevés were taken following the Braun-Blanquet phytosociological methodology. A statistical treatment was applied to establish a separation among Juniperus communities. To understand the presence of Juniperus communities in territories dominated by species in the Quercus genus, we applied Thornthwaite’s formula to calculate potential evapotranspiration. The general cluster analysis clearly distinguishes two groups of plant communities and separates the different associations in each group. All the plant communities growing on rocky crests and in extremely steep sloping areas are significantly influenced by the soil. The ombroclimatic index does not explain the presence of plant communities influenced by substrate, so we proposed a new ombroedaphoxeric index which explains the presence of Juniperus communities in territories with a thermotype between the thermo- and supramediterranean. The areas of distribution of Juniperus species are expanding due to the spread of rocky areas; this phenomenon causes an increase in edaphoxerophilous areas and a decrease in climatophilous ones. We propose four new plant associations, with updated structures and floristic compositions. Efficient conservation is possible in both the territories studied (Spain and Portugal) through the implementation of specific cross-border cooperation projects.

Description: Abstract In this study, the creep rate across the North Anatolian Fault was directly measured in the western Sea of Marmara using the seafloor acoustic ranging technique; the data reveal coupling conditions on the fault interface and stress accumulation with implications for regional seismic risk evaluation. Continuous measurements over a period of 3.5 years at a site in the Western High clearly indicate right‐lateral displacement at a rate of 10.7 ± 4.7 mm/year (95% confidence level); approximately half of the regional block motion at this location is released by this steady motion. A simple model of three elastic layers—a partially creeping sedimentary layer (8 km) at the top with the observed rate, a locked (3 km) and fully creeping layer in the middle, and a bottom layer—assumed from seismicity, reasonably explains onshore Global Navigation Satellite System data for the surrounding region.

Description: Floods are among the most destructive natural disasters, which are highly complex to model. The research on the advancement of flood prediction models contributed to risk reduction, policy suggestion, minimization of the loss of human life, and reduction of the property damage associated with floods. To mimic the complex mathematical expressions of physical processes of floods, during the past two decades, machine learning (ML) methods contributed highly in the advancement of prediction systems providing better performance and cost-effective solutions. Due to the vast benefits and potential of ML, its popularity dramatically increased among hydrologists. Researchers through introducing novel ML methods and hybridizing of the existing ones aim at discovering more accurate and efficient prediction models. The main contribution of this paper is to demonstrate the state of the art of ML models in flood prediction and to give insight into the most suitable models. In this paper, the literature where ML models were benchmarked through a qualitative analysis of robustness, accuracy, effectiveness, and speed are particularly investigated to provide an extensive overview on the various ML algorithms used in the field. The performance comparison of ML models presents an in-depth understanding of the different techniques within the framework of a comprehensive evaluation and discussion. As a result, this paper introduces the most promising prediction methods for both long-term and short-term floods. Furthermore, the major trends in improving the quality of the flood prediction models are investigated. Among them, hybridization, data decomposition, algorithm ensemble, and model optimization are reported as the most effective strategies for the improvement of ML methods. This survey can be used as a guideline for hydrologists as well as climate scientists in choosing the proper ML method according to the prediction task.

Description: Both the geometry and the depth of the seismogenic zone of the North Anatolian Fault under the Marmara Sea (the Main Marmara Fault; MMF) are poorly understood, in part because of the fault's undersea location. We recorded 10 months of microseismic data with a dense array of ocean bottom seismographs and then applied double-difference relocation and 3-D tomographic modeling to obtain precise hypocenters on the MMF beneath the central and western Marmara Sea. The hypocenters show distinct lateral changes along the MMF: (1) Both the upper and lower crust beneath the Western High are seismically active and the maximum focal depth reaches 26 km, (2) seismic events are confined to the upper crust beneath the region extending from the eastern part of the Central Basin to the Kumburgaz Basin, and (3) the magnitude and direction of dip of the main fault changes under the Central Basin, where there is also an abrupt change in the depth of the lower limit of the seismogenic zone. We attribute this change to a segment boundary of the MMF. Our data show that the upper limit of the seismogenic zone corresponds to sedimentary basement. We also identified several seismically inactive regions within the upper crust along the MMF; their spatial extent beneath the Kumburgaz Basin is greater than beneath the Western High. From the comparison with seafloor extensometer data, we consider that these regions might indicate zones of strong coupling that are accumulating stress for release during future large earthquakes.