ALBERT

Description: Meiofaunal biomass, despite its ecological importance, is rarely assessed in marine ecological studies due to time-consuming and costly indirect procedures or inaccurate direct methods. Here, we present a semi-automated image analysis method for obtaining the widths and lengths of photographed nematodes that employs the Leica LAS Image Analysis module and is used to estimate Nematoda (a dominant marine meiofaunal taxon) biomass. Samples of 100 individual nematodes from 10 replicate samples representing two different environments (muddy fjord bottom and seagrass-vegetated sands) were used for the analyses, which were performed manually and semi-automatically. Manual measurements were taken by three independent analysts to check for individual differences. The automated measurements were collected almost two times faster than the manual ones. The three analysts and the computer did not differ in their assessments of nematode length, but significant differences existed among the measurements of maximum width by the three analysts (due to different designations of the point of nematode maximum width required by the manual method). For estimating individual nematode biomass, a formula using the equation for the volume of cylinder (one of the three tested in the study) is recommended. The total nematode biomass values obtained with this method were comparable to those reported from similar habitats. This semi-automated method seems to be less biased and more time and cost effective compared with standard manual protocols. The wider application of this method should facilitate the more frequent inclusion of meiofaunal biomass assessments in marine benthic surveys.

Description: In this work, we present a reversible ferroelectric molecular switch controlled by an external electric field. The studied (2Z)-1-(6-((Z)-2-hydroxy-2-phenylvinyl)pyridin-3-yl)-2-(pyridin-2(1H)-ylidene)ethanone ( DSA ) molecule is polarized by two uniaxial intramolecular hydrogen bonds. Two protons can be transferred along hydrogen bonds upon an electric field applied along the main molecular axis. The process results in reversion of the dipole moment of the system. Static ab initio and on-the-fly dynamical simulations of the DSA molecule placed in an external electric field give insight into the mechanism of the double proton transfer (DPT) in the system and allow for estimation of the time scale of this process. The results indicate that with increasing strength of the electric field, the step-wise mechanism of DPT changes into the downhill barrierless process in which the synchronous and asynchronous DPTs compete with each other.

Description: Bisphenol A (BPA) is employed in the manufacturing of epoxy, polyester-styrene, and polycarbonate resins, which are used for the production of baby and water bottles and reusable containers, food and beverage packing, dental fillings and sealants. The study was designed to examine the effects of 8-week exposure (a full cycle of spermatogenesis) to BPA alone and in a combination with X-irradiation on the reproductive organs and germ cells of adult and pubescent male mice. Pzh:Sfis male mice were exposed to BPA (5, 10, and 20 mg/kg) or X-rays (0.05 Gy) or to a combination of both (0.05 Gy + 5 mg/kg bw BPA). The following parameters were examined: sperm count, sperm motility, sperm morphology, and DNA damage in male gametes. Both BPA and X-rays alone diminished sperm quality. BPA exposure significantly reduced sperm count in pubescent males compared to adult mice, with degenerative changes detected in seminiferous epithelium. This may suggest a higher susceptibility of germ cells of younger males to BPA action. Combined BPA with X-ray treatment enhanced the harmful effect induced by BPA alone in male germ cells of adult males, whereas low-dose irradiation showed sometimes protective or additive effects in pubescent mice.

Description: ABSTRACT Deregulation of serine and glycine metabolism, have been identified to function as metabolic regulators in supporting tumor cell growth. The role of serine and glycine in regulation of cancer cell proliferation is complicated, dependent on concentrations of amino acids and tissue-specific. D-serine and glycine are coagonists of N-methyl-D-aspartate receptor subunit GRIN1. Importantly, NMDA receptors are widely expressed in cancer cells and play an important role in regulation of cell death, proliferation and metabolism of numerous malignancies. The aim of the present work was to associate the metabolism of glycine and D-serine with the anticancer activity of 2-methoxyestradiol. 2-methoxyestradiol is a potent anticancer agent but also a physiological 17β- estradiol metabolite. In the study we have chosen two malignant cell lines expressing functional GRIN1 receptors, i.e. osteosarcoma 143B and breast cancer MCF7. We used MTS assay, migration assay, flow cytometric analyses, western blotting and immunoprecipitation techniques as well as molecular modeling studies. We have demonstrated the extensive crosstalk between the deregulated metabolic network and cancer cell signaling. Herein, we observed an anticancer effect of high concentrations of glycine and D-serine in osteosarcoma cells. In contrast, the amino acids when used at low, physiological concentrations induced the proliferation and migration of osteosarcoma and breast cancer cells. Importantly, the pro-cancergogenic effects of both glycine and D-serine where abrogated by the usage of 2-methoxyestradiol at both physiological and pharmacological relevant concentrations. The obtained data confirmed that 2-methoxyestradiol may be a physiological anticancer molecule. This article is protected by copyright. All rights reserved

Description: In this article, the results of an investigation into the atmospheric pressure conditions in the Arctic in the period from 1801 to 1920 are presented. For this period, which can be described as ‘early instrumental’, limited meteorological data exist from a network of regular stations. As a result, in order to get at least a rough idea of pressure conditions in the Arctic in the study period, data from different land and marine expeditions were collected. A total of 94 pressure series of monthly means have been gathered, the duration of which is usually less than 2 years. While the area and time periods covered by the data are variable, it is still possible to describe the general character of the pressure conditions. The results show that the areally averaged Arctic pressure in the early instrumental period (1861–1920) was 0.8 hPa lower than today (1961–1990). Lower values of atmospheric pressure were also observed in all study regions, excluding the Atlantic. The greatest negative differences (−2.1 hPa) have been found for the Canadian Arctic. The greatest changes between the historical and present times were noted in all winter months and in winter as a whole (−1.9 hPa), while in summer and autumn they were very small and their average differences came to − 0.1 and − 0.2 hPa, respectively. Comparison of historical and contemporary annual courses of atmospheric pressure in the whole of the Arctic and in its particular regions reveals general consonance. There is evidence to show that changes in Arctic atmospheric pressure during the whole study period were insignificant. Atmospheric pressure in the first International Polar Year (IPY) period (September 1882 to July 1883) was, on average, 1.4 hPa higher than in modern period (1961–1990). The greatest positive seasonal differences between historical and contemporary pressure values occurred in autumn (2.6 hPa), while the lowest were in winter (only 0.2 hPa). Spatial patterns of average annual and seasonal atmospheric pressure in the Arctic were very similar to present day ones. The pressure differences calculated between historical and modern mean monthly values show that almost all of them lie within one standard deviation from present long-term mean (1961–1990). Thus, this means that the atmospheric pressure in the early instrumental period was not significantly different to that of the present day. Recent, commonly used gridded datasets of the sea level atmospheric pressure (HadSLP2 and the 20th Century Reanalysis Project) reveal quite a large positive bias in the period 1850–1920 in comparison to the real data from the instrumental observations. Copyright © 2012 Royal Meteorological Society

Description: Seagrass meadows are highly productive habitats that can act as ‘blue carbon sinks’ in coastal ecosystems by facilitating sedimentation and trapping particles. However, the magnitude and occurrence of these effects may be species and density-dependent. The present study is the first estimation of seagrass sediment carbon sink in the temperate Zostera marina beds in the Baltic Sea. Several descriptors of organic matter characteristics, along with possible organic matter sources in the sediment were compared at vegetated and unvegetated bottoms. The 210 Pb dating of the sediment has been used for accumulation rate assessment. The photopigments and POC concentrations in sediments were higher in vegetated bottoms. The SIAR (Stable Isotopes in R) mixing model based on nitrogen and carbon stable isotope values, indicated higher percentages of organic matter originated from seagrass production in vegetated sediments (40-45%) compared to unvegetated ones (5–21%). The carbon stock in the upper 10 cm of the vegetated sediments ranged from 50.2 ± 2.2 to 228.0 ± 11.6 [g m -2 ] whereas the annual C accumulation amount from 0.84 ± 0.2 to 3.85 ± 1.2 [g m -2 y -1 ]. Our study shows that even the relatively weakly developed vegetation of the small temperate seagrass species enhance organic carbon concentration in the sediments. Estimated carbon stock was much lower than those reported for most of the seagrass meadows elsewhere and the carbon burial rates was the lowest ever reported. Evidently, the global calculations of sediment carbon stock should be reconsidered by taking into account density and species-related variability.

Description: Abstract Fjords have been recently recognized as hotspots of organic carbon (Corg) sequestration in marine sediments. This study aims to identify regional and local drivers of variability of Corg burial in north Atlantic and Arctic fjords. We provide a comparative quantification of Corg, δ13C, photosynthetic pigments content, benthic biomass, consumption, Corg accumulation and burial rates in sediments in six fjords (60 to 810N). Higher sediment Corg content in southern Norway reflected longer phytoplankton growth season and higher productivity. Higher contributions of terrestrial Corg were noted in temperate/southern Norway (dense land vegetation and high precipitation) and Arctic/Svalbard (glacial erosion) than in subarctic/northern Norway locations. Benthic biomass and carbon consumption were best correlated to δ13C and photosynthetic pigments content indicating control by quality rather than quantity of available food. Benthic faunal consumption did not seem to affect the variability in Corg burial. Regional environmental factors (water temperature, latitude) combined with local factors (Corg, grain size, pigment concentration) explained 94 % of Corg burial variability. Based on the present study and literature data on Corg content, origin and burial rates, the fjords were classified into four categories: temperate, subarctic, Arctic with and without glaciers. The variability in marine productivity, terrestrial inflows and carbon sequestration in fjords must be considered for global estimates of their role in blue carbon storage and for building scenarios of future changes in the course of climate warming.