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  • 1
    Electronic Resource
    Electronic Resource
    Organization of chloroplast ribosomal RNA genes and in vitro self-splicing activity of the large subunit rRNA intron from the green alga Chlorella vulgaris C-27 (1997)
    Springer
    Current genetics 31 (1997), S. 503-510 
    Details
    ISSN: 1432-0983
    Keywords: Key wordsChlorella ; rrn23 ; Self-splicing ; Group I-intron
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Sequencing of the rRNA gene (rrn) cluster of Chlorella vulgaris C-27 chloroplasts has revealed a striking organizational difference in comparison to another species of the same genus, Chlorella ellipsoidea C-87. The rrn23 gene in C. vulgaris is also split. However, the 815-bp intervening sequence in this gene has been identified as a group-I intron. An in vitro rrn23 transcript containing the entire intron and parts of flanking exon sequences is able to self-splice in vitro in the presence of GTP and Mg++. Accurate ligation of the exons has been confirmed by sequencing the cDNA of the spliced products. GTP labelling of total Chlorella RNA in vitro has revealed that the number of self-splicing RNAs present in Chlorella chloroplasts is limited compared to that found in other green algal species.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002940050237
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  • 2
    Electronic Resource
    Electronic Resource
    Capabilities of polymer-modified monodisperse colloidal silica particles as biomaterial carrier (1997)
    Springer
    Colloid & polymer science 275 (1997), S. 220-226 
    Details
    ISSN: 1435-1536
    Keywords: Key words Biomaterial carrier ; immunolatex ; protein immobilization ; monodisperse colloidal silica ; polymer modification
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract  Polymer modification of monodispersed colloidal silica (0.5 μm) with poly(maleic anhydride-co-styrene) (P(MA-ST)) and poly (maleic anhydride-co-methyl methacrylate) (P(MA-MMA)) and application of the composite particles to biomaterial carriers were investigated. The reaction of bovine serum albumin(BSA)-immobilized P(MA-MMA)/SiO2 with the anti-BSA antibody showed higher sensitivity in immunological agglutination test than BSA–P(MA-ST)/SiO2, though immobilization efficiency of BSA on P(MA-MMA)/SiO2 was lower than that on P(MA-ST)/SiO2. Alkaline phosphatase and glucose oxidase immobilized on the composite particles exhibited extremely low activities, but α-chymotrypsin immobilized on P(MA-MMA)/SiO2 and its derivative particles showed the relative activity of 12.5% and 16.1% to the native enzyme, respectively. Grafting of a hydrophilic polymer of poly(acrylic acid) to P(MA-ST)/SiO2 let to an increase of the immobilized α-chymotrypsin activity to give the maximum relative activity of 55.5%.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s003960050075
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  • 3
    Electronic Resource
    Electronic Resource
    Formation of colloid crystals from polymer-modified monodisperse colloidal silica in organic solvents (1999)
    Springer
    Colloid & polymer science 277 (1999), S. 479-482 
    Details
    ISSN: 1435-1536
    Keywords: Key words Colloid crystal ; Polymer-modified colloidal silica ; Organic media ; Maleic anhydride-styrene copolymer ; Polystyrene ; Poly(methyl methacrylate)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract The formation of colloid crystals from monodisperse and polymer-modified silica particles in organic solvents was investigated. Maleic anhydride–styrene copolymer-modified silica formed crystals in polar organic solvents, which dissolve the copolymer, while the original colloidal silica formed crystals in organic solvents which were miscible with water. The critical volume fraction in the crystal formation of the polymer-modified silica was lower than that from the unmodified silica in the same solvent. Polystyrene- and poly(methyl methacrylate)-modified silica particles also crystallized in organic solvents, but the features of the formation were different from those of poly(maleic anhydride-styrene)-modified particles.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s003960050412
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  • 4
    Electronic Resource
    Electronic Resource
    The modulatory effect of endogenous parathyroid hormone on the action of hydrochlorothiazide in pseudohypoparathyroidism type I (1994)
    Springer
    Calcified tissue international 54 (1994), S. 473-476 
    Details
    ISSN: 1432-0827
    Keywords: Pseudohypoparathyroidism ; Thiazide ; Kidney ; Bone ; Parathyroid hormone
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Abstract We compared the effect of orally administered 100 mg of hydrochlorothiazide (HCTZ) among eight patients with pseudohypoparathyroidism (PHP) type I, 11 patients with idiopathic hypoparathyroidism (IHP), and 12 patients with primary hyperparathyroidism (1oHPT). Patients with PHP type I or with IHP were studied during the treatment with 1α-hydroxylated metabolites of vitamin D3. HCTZ raised serum levels of calcium (Ca) in 1oHPT (P〈0.001) and PHP type I (P〈0.01) but did not increase urinary excretion of Ca. Serum parathyroid hormone (PTH) in PHP type I decreased (P〈0.02) after HCTZ administration in response to the increase in serum Ca. HCTZ did not raise serum levels of Ca in IHP but increased urinary excretion of Ca in this group (P〈0.01). HCTZ suppressed tubular reabsorption of phosphate (P) in IHP (P〈0.01) and 1oHPT (P〈0.05) but not in PHP type I. Urinary excretion of cAMP did not change after HCTZ administration in PHP type I, IHP, or 1oHPT. Endogenous PTH modulated the effects of HCTZ on Ca mobilization from bone and renal reabsorption of Ca in PHP type I with normal or high serum levels of PTH and in 1oHPT with high serum levels of PTH. The inhibitory effect of HCTZ on renal tubular reabsorption of P (probably from proximal tubules) was independent of PTH. The resistance to this inhibitory effect of HCTZ on P reabsorption in PHP type I suggested a proximal tubular dysfunction in this disorder.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00334326
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  • 5
    Electronic Resource
    Electronic Resource
    The effect of endogenous parathyroid hormone, exogenous calcitonin, and dibutyryl cyclic AMP on urinary excretion of N-acetyl-β-D-glucosaminidase (1994)
    Springer
    Calcified tissue international 54 (1994), S. 186-194 
    Details
    ISSN: 1432-0827
    Keywords: Glucosaminidase ; Parathyroid hormone ; Calcnonin ; Cyclic AMP ; Urine
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Abstract Urinary excretion of N-acetyl-β-D-glucosaminidase (NAG) transiently increases after PTH(1–34) infusion in idiopathic hypoparathyroidism (IHP) but this response is impaired in pseudohypoparathyroidism (PHP) type I. We investigated the effects of endogenous PTH, exogenous calcitonin (CT), and dibutyryl cAMP (DBcAMP) on urinary excretion of NAG. Urinary NAG excretion in 14 patients with primary hyperparathyroidism (1oHPT) was more than in normal subjects (P〈0.001) and decreased after parathyroidectomy (P〈0.01). Urinary NAG excretion increased after the infusion of 1.5 MRC/kg of eel CT in eight normal subjects (P〈0.001), two patients with IHP, and a patient with PHP type Ib but not in a patient with PHP type Ia. The increases of urinary NAG excretion by CT and by PTH(1–34) were positively correlated with the increases of urinary cAMP excretion (r=0.752; P〈0.001 and r=0.534; P〈0.002, respectively). Urinary NAG excretion increased after DBcAMP infusion in five normal subjects (P〈0.01), two patients with IHP, and two with PHP type I. The increase of urinary NAG by 6.0 mg/kg of DBcAMP was more than by 2.5 mg/kg of DBcAMP in normal subjects (P〈0.01). The increase of urinary NAG by 2.5 mg/kg of DBcAMP in PHP type I was comparable with that by 6.0 mg/kg in normal subjects, suggesting a hyperresponsiveness to DBcAMP in PHP type I. Urinary excretion of NAG is a useful indicator of renal tubular responsiveness to PTH and CT. Cyclic AMP-dependent mechanism is probably involved in PTH and CT-induced increase in urinary excretion of NAG.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00301676
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  • 6
    Electronic Resource
    Electronic Resource
    Resetting of parathyroid hormone secretion after vitamin D3 treatment in hypoparathyroidism and after parathyroid adenectomy in primary hyperparathyroidism (1995)
    Springer
    Calcified tissue international 57 (1995), S. 30-34 
    Details
    ISSN: 1432-0827
    Keywords: Parathyroid hormone ; Hypoparathyroidism ; Vitamin D3 ; Hyperparathyroidism ; Parathyroidectomy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Abstract The relationship between parathyroid hormone (PTH) secretion and extracellular calcium (Ca) level is reciprocal causality. The equilibrium operating point determines basal PTH secretion rate and basal extracellular Ca level. We studied how this equilibrium was achieved in the subjects with decreased PTH secretion or decreased parathyroid glands number. Basal/maximum ratio of serum PTH, which reflects the basal secretory state of parathyroid glands, was increased in 9 hypoparathyroid patients treated with vitamin D3 (VD3) [7 patients with idiopathic hypopar-athyroidism (IHP), and two patients with postsurgical hypoparathyroidism] and in seven of nine parathyroid adenectomized patients. There was a negative correlation between the ratio and basal serum Ca level in the patients with IHP after VD3 treatment (r=0.7167, P〈0.05) and in the patients after parathyroid adenectomy (r=0.7760, P〈0.05). The regression curves in these two groups coincided regardless of the difference in maximum PTH secretion rate, which suggested that the basal secretory state of parathyroid glands was determined by extracellular Ca level in a similar manner in these subjects. There was a sigmoidal relationship between basal/maximum ratio of serum PTH and basal serum Ca level, when the data were collected from 15 hypoparathyroid patients before or after VD3 treatment, 9 parathyroid adenectomized patients, and 10 normal subjects (r=0.9057, P〈0.001). This sigmoidal curve is thought to represent the fundamental relationship between the basal secretory state of parathyroid glands and extracellular Ca level.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00298993
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  • 7
    Electronic Resource
    Electronic Resource
    Evidence for the renal origin of urinary kinin (1964)
    Springer
    Cellular and molecular life sciences 20 (1964), S. 396-397 
    Details
    ISSN: 1420-9071
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Zusammenfassung Nach intravenöser Kallikrein- oder Bradykinininfusion wurde an den Patienten keine signifikante Zunahme der Kininausscheidung beobachtet. Bradykinininfusion in die Nierenarterien des Hundes führte zu keiner Vermehrung der Kininexkretion im Harn. Die Ergebnisse bestätigen, dass Kinin im Harn nicht aus dem zirkulierenden Blut stammt, sondern von den Tubulusepithelien gebildet und sezerniert wird.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02147988
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  • 8
    Electronic Resource
    Electronic Resource
    Circulating plasma kinin in patients with bronchial asthma (1967)
    Springer
    Cellular and molecular life sciences 23 (1967), S. 626-627 
    Details
    ISSN: 1420-9071
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Zusammenfassung Bei 33 Patienten mit Asthma bronchiale von verschiedenem Erkrankungsgrade wurde der Kiningehalt im zirkulierenden Blut bestimmt. Erhöhte Blutkininwerte wurden in den Patienten gefunden, und zwar im allgemeinen mit der Schwere der Krankheit korrelierbar. Daraus folgt, dass das «Kinin» aetiologisch mit Asthma bronchiale verknüpft ist.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02144161
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  • 9
    Electronic Resource
    Electronic Resource
    An improved bioassay method for kinins (1968)
    Springer
    Cellular and molecular life sciences 24 (1968), S. 455-457 
    Details
    ISSN: 1420-9071
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Zusammenfassung Unter Ausnützung der besonderen Empfindlichkeit der Femoralarterie des Kaninchens für Kinin wurde die autoperfusionierte Hinterextremität beobachtet. Intraarterielle Injektion von 0,5 ng synthetisiertem Bradykinin ergab eine messbare, dosisabhängige Vasodilatation in fast allen Versuchstieren. Dieses Kreislaufpräparat erweist sich zur Kinin-Bestimmung als besonders geeignet.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02144388
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  • 10
    Electronic Resource
    Electronic Resource
    Fertile fruit trees obtained by somatic hybridization: navel orange (Citrus sinensis) and Troyer citrange (C. sinensis x Poncirus trifoliata) (1991)
    Springer
    Theoretical and applied genetics 81 (1991), S. 141-143 
    Details
    ISSN: 1432-2242
    Keywords: Somatic hybrids ; Navel orange ; Troyer citrange ; Plant breeding
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Nucellar cell suspension protoplasts of navel orange (Citrus sinsensis Osb.) were chemically fused with mesophyll protoplasts of Troyer citrange (C. sinensis x Poncirus trifoliata) and cultured in hormone-free Murashige and Tucker medium containing 0.6 M sucrose. Two types of plant were regenerated through embryogenesis. One type showed intermediate mono-and difoliate leaves and the other types was identical to Troyer citrange. The regenerated plants with intermediate morphology were demonstrated by chromosome counts and rDNA analysis to be amphidiploid somatic hybrids. Five clones of these somatic hybrids were grafted in the field. After 4 years, they set flowers having a morphology intermediate between those of the two parents. The pollen grains showed high stainability and sufficient germinability, and were larger than those of Troyer citrange. The fruits of the somatic hybrids were large and spherical with thick rinds. Most of them contained seeds with normal germinability. These results indicate that somatic hybridization is a useful tool for Citrus breeding.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00215714
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