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  • 1
    Electronic Resource
    Electronic Resource
    Mechanosensitive Channels in the Cell Body of Chlamydomonas (1998)
    Springer
    The journal of membrane biology 166 (1998), S. 149-155 
    Details
    ISSN: 1432-1424
    Keywords: Key words: Mechanosensitive channel —Chlamydomonas— Calcium — Flagella — Patch clamp
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract. Mechanosensitive channels appear ubiquitous but they have not been well characterized in cells directly responding to mechanical stimuli. Here, we identified tension-sensitive channel currents on the cell body of Chlamydomonas, a protist that shows a marked behavioral response to mechanical stimulation. When a negative pressure was applied to the cell body with a patch clamp electrode, single-ion-channel currents of 2.4 pA in amplitude were observed. The currents were inhibited by 10 μm gadolinium, a general blocker of mechanosensitive channels. The currents were most likely due to Ca2+ influxes because the current was absent in Ca2+-free solutions and the reversal potential was 98 mV positive to the resting potential. The distribution of channel-open times conformed to a single exponential component and that of closed times to two exponential components. This mechanosensitive channel was similar to the one found in the flagella in the following respects: both channels were inhibited by Gd3+ at 10 μm but not at 1 μm; both passed Ca2+ and Ba2+; their kinetic parameters for channel opening were similar. These observations raise the possibility that identical mechanosensitive channels may function both in the behavioral control through the mechanoreception by the flagella and in the regulation of cellular physiology in response to mechanical perturbation on the cell body.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002329900456
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  • 2
    Electronic Resource
    Electronic Resource
    Preparation and properties of SiO2-TiO2 thin films from silicic acid and titanium tetrachloride (1994)
    Springer
    Journal of sol gel science and technology 1 (1994), S. 185-191 
    Details
    ISSN: 1573-4846
    Keywords: silicic acid ; titanium tetrachloride ; SiO2-TiO2 ; sol solution ; dip coating ; thin film
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract The preparation of SiO2-TiO2 thin films by the sol-gel method using silicic acid and titanium tetrachloride as starting materials was studied. The homogeneous sols were obtained by the condensation reaction of silicic acid with titanium tetrachloride in methanol-tetrahydrofuran. The dipcoating of slide glasses and silicon wafers followed by heat treatment gave oxide thin films of 88–93% transmittance, 3000–4500 Å thickness, and 1.45–1.80 refractive index, depending on heat-treatment temperature and TiO2 content. FT-IR measurement showed that the Si-O-Ti bond is formed even in the sol and films. The variations of film thickness and refractive index on transformation from the gels into the oxides were found to be quite low.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00490248
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  • 3
    Electronic Resource
    Electronic Resource
    Simultaneous determination of trace metals by solid phase absorptiometry: application to flow analysis of some rare earths (2000)
    Springer
    Fresenius' journal of analytical chemistry 368 (2000), S. 443-448 
    Details
    ISSN: 1432-1130
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract Solid phase absorptiometry was applied to the simultaneous flow analysis of trace metals in combination with a micro black flow-through cell packed with ion exchanger beads and a multi-channel photodetector connected with optical fibers. A 4.0 cm3 sample solution containing five rare earth metal ions (Dy, Ho, Tm, Er and Nd) was introduced into a flow system and these metal ions were concentrated on a cation exchanger (Muromac 50W-X4) in a flow-through cell. The absorbance increases originating from the f-f or d-f transition bands of these metal ions were directly and continuously measured at 910 nm for Dy, at 530 nm for Ho, at 683 nm for Tm, 522 nm for Er and at 790 nm for Nd, respectively. A multi-variable analysis method was combined with the flow analysis because the absorption spectra of the five rare earth metal ions partly overlapped one another. Although no coloring reagents were used, the proposed method was about ¶200 times more sensitive than the corresponding solution method. The reproducibility of this method was less than ± 5%. The detection limits were 0.03, 0.40, 0.30, 0.35 and 0.23 mg dm–3 for Dy, Ho, Tm, Er and Nd, respectively. Five rare earth metal ions could be precisely determined in practical samples such as yttrium concentrate.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002160000515
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  • 4
    Electronic Resource
    Electronic Resource
    Abstracts of the Twenty-second Annual Meeting of the Japanese Society of Biometeorology, Kanazawa, 28–29 October 1983 (1985)
    Springer
    International journal of biometeorology 29 (1985), S. 361-384 
    Details
    ISSN: 1432-1254
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geography , Physics
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02189212
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  • 5
    Electronic Resource
    Electronic Resource
    A possible association of Y chromosome heterochromatin with stature (1981)
    Springer
    Human genetics 〈Berlin〉 58 (1981), S. 268-270 
    Details
    ISSN: 1432-1203
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary A correlation between Y chromosome length and stature was statistically analyzed in a normal male population of 142 Japanese students with a mean age of 24.0 years. Evidence was obtained that increased length of the heterochromatic band Yq12 may be associated with increased height: The correlation coefficient between band Yq12 length and height was 0.17, statistically significant at the 5% level. And, taller males had longer Y chromosomes, in which the mean length of band Yq12 was significantly longer than that of shorter males. No correlation was seen between length of the euchromatic band Yq11 and stature. The present study reveals a possible effect of Yq heterochromatin on the development of body height in man.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00294920
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  • 6
    Electronic Resource
    Electronic Resource
    Negative association between asthma and variants of CC16(CC10) on chromosome 11q13 in British and Japanese populations (1998)
    Springer
    Human genetics 〈Berlin〉 103 (1998), S. 57-59 
    Details
    ISSN: 1432-1203
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The gene encoding Clara cell-derived inflammatory molecule CC16 has been cited as a candidate gene for atopic asthma on chromosome 11q13. A genetic association study was performed with variants of the CC16 gene on chromosome 11q13 in relation to asthma in British (n=275) and Japanese (n=300) populations. No significant association was found between asthma and CC16 genotypes, irrespective of atopic status in these two populations. These data suggest that CC16 might not be the major locus for asthma on 11q13.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004390050783
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  • 7
    Electronic Resource
    Electronic Resource
    Molecular phylogeny of Dipterocarpaceae in Southeast Asia using RFLP of PCR-amplified chloroplast genes (1996)
    Springer
    Theoretical and applied genetics 93 (1996), S. 22-29 
    Details
    ISSN: 1432-2242
    Keywords: Dipterocarpaceae ; PCR-RFLP Chloroplast gene ; Phylogeny ; Southeast Asia
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Dipterocarpaceae is the dominant family of Southeast Asia's climax tropical rain forest region, and it contains the region's most important commercial timber species. A molecular phylogeny of the Dipterocarpaceae subfamily Dipterocapoideae was constructed using restriction fragment length polymorphisms of polymerase chain reaction-amplified specific genes in chloroplast DNA. A total of 141 site changes were detected among ten genera and 30 species in 11 different genes: rbcL, psbA, psbD, rpoB, rpoC, petB, atpH, 16S, psaA, petA and trnK. Phylogenetic trees constructed by Wanger parsimony and neighbor-joining methods, using Upuna as the outgroup, displayed five monophytelic groups that included Upuna: HopeaShorea-Parashorea-Neobalanocarpus; Dryobalanops; Dipterocarpus; Anisoptera-Vatica-Cotylelobium; and Upuna. The phylogenetic trees clearly separate species with two different base chromosome numbers: the first group is x=7, and the other is x=11. The x=7 group is thought to be in a synapomorphic character state. Parashorea lucida is a sister to most Shorea species. Neobalanocarpus heimii and Hopea from a clade of a sister to two Shorea species, and Cotylelobium and Vatica are closely related species. Our conclusions agree with a phylogeny derived from wood anatomy data analysis, and with Symington's and Ashton's taxonomic classifications.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00225722
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  • 8
    Electronic Resource
    Electronic Resource
    Molecular phylogeny of Dipterocarpaceae in Southeast Asia using RFLP of PCR-amplified chloroplast genes* (1996)
    Springer
    Theoretical and applied genetics 93 (1996), S. 22-29 
    Details
    ISSN: 1432-2242
    Keywords: Key words Dipterocarpaceae  ;  PCR-RFLP Chloroplast gene  ;  Phylogeny  ;  Southeast Asia
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Dipterocarpaceae is the dominant family of Southeast Asia’s climax tropical rain forest region, and it contains the region’s most important commercial timber species. A molecular phylogeny of the Dipterocarpaceae subfamily Dipterocapoideae was constructed using restriction fragment length polymorphisms of polymerase chain reaction-amplified specific genes in chloroplast DNA. A total of 141 site changes were detected among ten genera and 30 species in 11 different genes: rbcL, psbA, psbD, rpoB, rpoC, petB, atpH, 16S, psaA, petA and trnK. Phylogenetic trees constructed by Wanger parsimony and neighbor-joining methods, using Upuna as the outgroup, displayed five monophytelic groups that included Upuna: HopeaShorea-Parashorea-Neobalanocarpus; Dryobalanops; Dipterocarpus; Anisoptera-Vatica-Cotylelobium; and Upuna. The phylogenetic trees clearly separate species with two different base chromosome numbers: the first group is and the other is The group is thought to be in a synapomorphic character state. Parashorea lucida is a sister to most Shorea species. Neobalanocarpus heimii and Hopea from a clade of a sister to two Shorea species, and Cotylelobium and Vatica are closely related species. Our conclusions agree with a phylogeny derived from wood anatomy data analysis, and with Symington’s and Ashton’s taxonomic classifications.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220050242
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  • 9
    Electronic Resource
    Electronic Resource
    Sequence-tagged-sites (STSs) of cDNA clones in Cryptomeria japonica and their evaluation as molecular markers in conifers (1997)
    Springer
    Theoretical and applied genetics 94 (1997), S. 764-772 
    Details
    ISSN: 1432-2242
    Keywords: Key words Linkage map ; RFLP ; STS ; Conifers ; Cryptomeria japonica ; cDNA ; Polymorphism ; Phylogeny ; Homology
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  We have generated 66 sequence-tagged-site (STS) markers from cDNA clones of Cryptomeria japonica, and 60% of them have already been mapped into C. japonica linkage groups. All of the STS markers showed a single fragment following polymerase chain reaction (PCR) amplification. We investigated by polymorphism of these STS markers in a mapped F2 population and 15 plus trees by means of a restriction endonuclease analysis. Polymorphism levels were 10.6% and 22.7% in the F2 population and the 15 plus trees, respectively. PCR amplification levels of the 66 STS markers in 14 conifer species varied depending on their genetic relationship with C. japonica. Taxodium, which is closely related to C. japonica, had the most amplifications (31.82%), followed by Sequoiadendron giganteum, which is of the same family. The average proportion of PCR amplifications in each family gradually declined in the following order: from Taxodiaceae to Cuppresaceae, Sciadopityaceae, Pinaceae, and Taxaceae. These results are in general agreement with a molecular phylogenetic relationship based on chloroplast DNA. The 66 STS markers will be useful as on anchor point for genome mapping and population genetics, and some of them will also be useful when studying other conifers.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220050476
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  • 10
    Electronic Resource
    Electronic Resource
    Molecular phytogeny of conifers using RFLP analysis of PCR-amplified specific chloroplast genes (1995)
    Springer
    Theoretical and applied genetics 91 (1995), S. 1222-1236 
    Details
    ISSN: 1432-2242
    Keywords: Molecular phylogeny ; Chloroplast genes ; Conifers ; RFLP (restriction fragment length polymorphism) ; PCR (polymerase chain reaction)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We investigated the molecular phylogeny of conifers using restriction endonuclease fragment length polymorphism of six polymerase chain reaction-amplified chloroplast genes — frxC, rbcL, psbA, psbD, trnK, and 16S. We detected 227 total site changes among species, representing 23, 26, 38, 48, 67, and 25 site changes in frxC, psbA, psbD, rbcL, trnK and 16S, respectively. The mean nucleotide substitution was 10.75% (SD 0.573) among species in five families. Forty maximally parsimonious trees were obtained using the Wagner parsimony method, and a 50% majority-rule consensus tree was obtained from them. Data analysis produced similar basic patterns when both the Wagner parsimony and the neighbor-joining methods were applied, and the main lineages were clearly separated. Taxaceae and Cephalotaxaceae species were used as the out-groups when applying Wagner parsimony methods. With the Wagner method, the consistency index was 0.510, the retention index was 0.879, and tree length was 435 steps. Our results indicated that Cupressaceae and Taxodiaceae are closely related families and that Sciadopitys verticillata is the basal lineage of Cupressaceae and Taxodiaceae. The neighbor-joining tree is similar to the 50% majority-rule consensus of the 40 Wagner parsimony trees except for the position of Keteleeria daversifolia, the Picea and Cedrus group, and the divergence within Cupressaceae.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00220933
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