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  • 1
    Electronic Resource
    Electronic Resource
    Nonenzymatic Template-Directed Condensation of Short-Chained Oligouridylates on a Poly(A) Template (2000)
    Springer
    Origins of life and evolution of the biospheres 30 (2000), S. 503-511 
    Details
    ISSN: 1573-0875
    Keywords: nonenzymatic template-directedcondensation ; oligouridylates ; poly(A)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences
    Notes: Abstract An oligouridylate with chain-length of more thanthree was condensed on a polyadenylate [poly(A)]template with 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDAC) in imidazole buffer. The condensation reactionproceeds via the phosphorimidazolide of theoligouridylate as an intermediate. Pentauridylate[(pU)5] was converted to decauridylate[(pU)10] in 10% yield at 0 °C for 7 daysin the presence of the poly(A) template, while nocoupling product was obtained in the absence of thepoly(A) template. The resulting linkage of the(pU)10 was mainly 2′–5′ linkage.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1026583814064
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