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  • Springer  (68)
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  • 1
    Electronic Resource
    Electronic Resource
    Favourable interaction between heavy and light chains arrests the undesirable oligomerization of heavy chains in the refolding of denatured and reduced immunoglobulin G (1997)
    Springer
    Cellular and molecular life sciences 53 (1997), S. 929-934 
    Details
    ISSN: 1420-9071
    Keywords: Key words. Aggregation; H chain; immunoglobulin G; L chain; protein folding; slow dialysis.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract. Recently we developed a slow dialysis method that effectively refolds denatured and reduced immunoglobulin G (IgG) [Maeda, Ueda and Imoto (1996) Prot. Engng 9: 95-100]. This method allows both individual and simultaneous refolding of denatured and reduced H and L chains. Analysis by SDS-polyacrylamide gel electrophoresis revealed that some oligomers were formed through disulfide bonds when H chains were refolded individually. It was also shown that the extent of IgG obtained by rejoining the mixture of refolded H and L chains which had been refolded individually was similar to that obtained by refolding denatured and reduced whole IgG. The results indicated that a favourable interaction between H and L chains prevented formation of H-chain oligomers to yield intact IgG. The present results suggest a mechanism whereby individually folded chains might associate to form IgG molecules in vivo.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s000180050113
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  • 2
    Electronic Resource
    Electronic Resource
    Interannual, seasonal, local and body size variations in reproduction of the prawn Penaeus (Marsupenaeus) japonicus (Crustacea: Decapoda: Penaeidae) in the Ariake Sea and Tachibana Bay, Japan (2000)
    Springer
    Marine biology 136 (2000), S. 223-231 
    Details
    ISSN: 1432-1793
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  The reproductive biology of female Penaeus japonicus Bate was investigated in the Ariake Sea and Tachibana Bay (located outside the Ariake Sea), Japan from 1994 to 1996. Interannual, seasonal, individual female body size and spatial influences on the incidence of spawning were examined. The proportion of inseminated females, increased with increasing body size up to 170 mm body length (BL) and decreased thereafter. The minimum size at maturity was similar between years; 130 to 140 mm BL. The minimum size of individuals with developing ovaries or spermatophores differed from that of ripe females by 20 to 25 mm. Spawning occurred in the central part of the Ariake Sea and Tachibana Bay but rarely in the inner part. P. japonicus had a clear reproductive cycle. Spawning started earlier and ended later, occurring from mid-May to mid-October, in the eastern central part of and outside of the Ariake Sea compared with the western central part, where spawning occurred from mid-June to mid-September. Spawning dynamics differed across seasons, body sizes and areas but not across years. The seasonal peak in the proportion of ripe females varied with body size. In small individuals (130 to 169 mm BL), no peak was observed, whereas in large individuals (〉170 mm BL) the proportion of ripe individuals peaked in June. The proportion of ripe individuals increased with increasing body size and was high outside the Ariake Sea. Factors causing the variation in spawning dynamics are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002270050680
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  • 3
    Electronic Resource
    Electronic Resource
    Effect of strong wall heating on turbulence statistics of a channel flow (1994)
    Springer
    Experiments in fluids 18 (1994), S. 87-94 
    Details
    ISSN: 1432-1114
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract Turbulence statistics of a channel flow with strong wall heating at Reynolds number of 14, 000 were investigated experimentally. The statistical quantities up to triple correlations were estimated, using data measured by a single component LDV. The wall temperature varies up to 1000 K. Rms values of streamwise and cross-stream velocities and their correlation decrease in the thermal boundary layer except near the wall. Near the wall, the ejection of low-speed fluid outward from the wall is intensified due to thermal expansion, and it cancels the decrease in statistical quantities. As a result, the statistical quantities do not vary apparently.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00209364
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  • 4
    Electronic Resource
    Electronic Resource
    Velocity statistics along the stagnation line of an axi-symmetric stagnating turbulent flow (1997)
    Springer
    Experiments in fluids 22 (1997), S. 473-481 
    Details
    ISSN: 1432-1114
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract  Velocity statistics along the stagnation line of an axi-symmetric wall stagnating turbulent flow are studied experimentally. A low turbulence, uniform air flow from a nozzle type air supply with an exit diameter of 50 mm stagnates at a wall located 50 mm downstream. A flow velocity is set to 3 m/s, 10 mm downstream from the exit of the air supply. Instantaneous values of streamwise and radial velocities are measured by laser-Doppler velocimetry. The turbulence level in the air flow is changed by use of turbulence generator. When the turbulence generator is not installed in the air supply, the mean velocity profile in the streamwise direction fits well with that of a laminar viscous flow with the rms value of velocity fluctuations low near the wall. With the turbulence generator installed, a significant turbulence structure appears near the wall. When the wall is approached, the rms value of velocity fluctuations in the streamwise direction decreases monotonically while the profile of the rms value in the radial direction reaches a maximum near the wall. The increase in the rms value of velocity fluctuations in the radial direction near the wall is attributed to the bi-modal histogram of the fluctuating velocity in the radial direction. Near the wall, the instantaneous stagnation streamline fluctuates and the probability of the mean location of the stagnation point reaches a maximum not at the stagnation line but on a circle around the stagnation line, resulting in the bi-modal histogram. Turbulence statistics, the rms value of velocity fluctuation and the turbulent kinetic energy, can be normalized successfully by similarity parameters based on the strain rate and the reference turbulent kinetic energy introduced by Champion and Libby.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s003480050074
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  • 5
    Electronic Resource
    Electronic Resource
    Velocity-temperature correlation in strongly heated channel flow (1995)
    Springer
    Experiments in fluids 18 (1995), S. 454-461 
    Details
    ISSN: 1432-1114
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract Velocity-temperature correlations in a strongly heated channel flow were investigated experimentally by a LDV and a resistance thermometer. The wall heat flux is varied up to 50,000 W/m2 with reference mean-velocity of 15 m/s, and then, the wall temperature reaches up to 1,000 K. The results show that the ejection fluid motion is intensified by the strong heating near the wall increasing the turbulent heat flux from the wall. The intensification of ejection motion balances the destruction of turbulent heat flux. Then, the overall turbulent heat transfer does not change clearly.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00208469
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  • 6
    Electronic Resource
    Electronic Resource
    Structure of turbulent two-dimensional channel flow with strongly heated wall (1992)
    Springer
    Experiments in fluids 13 (1992), S. 17-25 
    Details
    ISSN: 1432-1114
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract A laser Doppler velocimeter and a resistance thermometer were used to study velocity and temperature statistics in a strongly heated turbulent two-dimensional channel flow, with the wall temperature up to 700 °C and a Reynolds number of 14,000. Normalized mean velocity and mean temperature profiles were not significantly affected by the wall heating. Turbulent intensities of temperature fluctuation were also insensitive to the heat flux. However, turbulent intensities of velocity fluctuation were suppressed in the region away from the wall, whereas those near the wall were not changed noticeably by the wall heating. This phenomenon was explained by the balance of three parameters: turbulent production, viscous dissipation and intermittency.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00208070
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  • 7
    Electronic Resource
    Electronic Resource
    Turbulence structure in an annuli with strongly heated inner cylinder (1999)
    Springer
    Experiments in fluids 27 (1999), S. 137-144 
    Details
    ISSN: 1432-1114
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract This structure of turbulent flow in an annulus with strong inner cylinder wall heating has been studied in terms of velocity and temperature with wall temperatures up to 707 °C and a Reynolds number of 48,000. With increase in wall heating, the turbulence very close to the wall was suppressed due to an increase in the kinematic viscosity. In the inner region, the intermittent mixing became intensive and the turbulent intensity increased whereas, in the outer region, the turbulence was suppressed since intermittent mixing was no longer effective. The results show that the thermal structure can be considered in terms of a passive scalar for wall temperatures less then around 200 °C, except in the leading region of the heated area.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s003480050338
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  • 8
    Electronic Resource
    Electronic Resource
    Multidentate phenyl-bonded phases in high-performance liquid chromatography (1992)
    Springer
    Chromatographia 34 (1992), S. 381-385 
    Details
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Molecular recognition ; Multidentate phenyl-bonded phases ; PAHs
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary New multidentate phenyl-bonded phases (MPBPs) were synthesized and evaluated the chromatographic retention behaviour with polycyclic aromatic hydrocarbons as sample probes in high-performance liquid chromatography. The new MPBPs show different retention characteristics from the previously synthesized MPBP, designated TP. Nevertheless, the results indicate that the retention mechanism is still the same as the TP phase: the size and shape of the solute molecule can be recognized by a cavity-like space formed by the methyl groups and phenyl rings of MPBPs.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02268372
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  • 9
    Electronic Resource
    Electronic Resource
    Aerodynamic structure of a flat plate laminar boundary layer with diffusion flame (1989)
    Springer
    Computational mechanics 5 (1989), S. 263-272 
    Details
    ISSN: 1432-0924
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract An aerodynamic structure of a laminar boundary layer over a flat plate with uniform fuel injection from the flat plate and with diffusion flame is investigated numerically. Elliptic type conservation equations are used to take into account the pressure variation within the boundary layer. Velocities and the pressure are solved numerically by ‘SIMPLER’ algorithm. One step irreversible chemical reaction of methane is assumed. An Arrhenius type chemical reaction rate model is assumed and the pre-exponential factor is varied from 1.0 × 1012 to 1.0 × 1030 m3/(kg ▪ s) as a parameter of the reactivity in order to elucidate the effect of the reactivity on the structure of the boundary layer. When the chemical reaction is very fast, the leading edge of the reaction zone reaches the flat plate. As the chemical reaction rate is decreased with a decrease in the pre- exponential factor, the leading edge of the reaction zone parts from the flat plate and it shifts downstream. The fuel is injected in front of the leading edge of the reaction zone, where the air is dominant, and the oxygen penetrates into the fuel dominant zone through the region between the leading edge and the flat plate. As a consequence, a premixed gas is formed around the leading edge of the reaction zone. The premixed gas seems to react in the region apart from the main visible flame.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01046943
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  • 10
    Electronic Resource
    Electronic Resource
    Histochemical analysis of sialic acids in the epididymis of the rat (1998)
    Springer
    Histochemistry and cell biology 109 (1998), S. 399-407 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract  A variety of sialic acids contained in the rat epididymis were histochemically examined by means of lectin and pre-lectin methods by light microscopy. Epididymides from adult male Sprague-Dawley rats were fixed in Bouin’s fluid and routinely embedded in paraffin wax. Hydrated sections were subjected either to the lectin methods using biotinylated Limax flavus, Sambucus nigra, Sambucus sieboldiana or Maackia amurensis lectins or to the selective periodate oxidation–phenylhydrazine–thiocarbohydrazide–silver protein–physical development technique with or without saponification. The present results revealed that principal cells in the initial segment and caput contain sialic acid linked to α2,6-galactose/N-acetylgalactosamine, whereas those in the corpus and cauda include the sialic acidα2,3-galactose sequence. Narrow and clear cells involve all the types of sialic acids examined. Basal and halo cells mainly contain sialic acidα2,3-galactose. 8- And/or 9-O-acetylated sialic acids were predominantly distributed in principal cells of the initial segment and proximal caput. These findings are taken to indicate that various sialic acids in the epididymis could participate in different physiological functions characteristic of the regions in this organ.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004180050241
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