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  • 1
    Electronic Resource
    Electronic Resource
    Genomic organization and chromosomal localization of the human casein gene family (1997)
    Springer
    Human genetics 〈Berlin〉 99 (1997), S. 368-373 
    Details
    ISSN: 1432-1203
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Five yeast artificial chromosome (YAC) clones containing the human casein gene family were isolated and characterized to study the control mechanisms for the expression of these genes. Partial restriction analysis in conjunction with the chromosomal fragmentation method and fluorescence in situ hybridization (FISH) analysis were performed to construct a detailed physical map of the casein gene family and to determine the chromosomal localization of these genes. The isolated YAC clones 748F3, 750D11, 882G11, 886B3 and 960D2 were 1.2 Mb, 860 kb, 800 kb 1.5 Mb and 1.5 Mb in size, respectively. The clones 748F3, 882G11, 886B3 and 960D2 contained the entire casein gene family, while the κ-casein gene was absent in 750D11. The human αS1-, β- and κ-casein genes were found to be closely linked and arranged in the order αS1-β-κ. The distance between αS1 and β, and between αS1 and κ was approximately 10 and 300 kb, respectively. The β-casein gene was oriented in the opposite direction to the αS1- and κ-casein genes. The casein gene family was localized to chromosome 4q21.1 by FISH analysis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004390050374
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  • 2
    Electronic Resource
    Electronic Resource
    Construction of an engineered yeast with glucose-inducible emission of green fluorescence from the cell surface (2000)
    Springer
    Applied microbiology and biotechnology 54 (2000), S. 90-96 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract An engineered yeast with emission of fluorescence from the cell surface was constructed. Cell surface engineering was applied to display a visible reporter molecule, green fluorescent protein (GFP). A glucose-inducible promoter GAPDH as a model promoter was selected to control the expression of the reporter gene in response to environmental changes. The GFP gene was fused with the gene encoding the C-terminal half of α-agglutinin of Saccharomyces cerevisiae having a glycosylphosphatidylinositol anchor attachment signal sequence. A secretion signal sequence of the fungal glucoamylase precursor protein was connected to the N-terminal of GFP. This designed gene was integrated into the TRP1 locus of the chromosome of S. cerevisiae with homologous recombination. Fluorescence microscopy demonstrated that the transformant cells emitted green fluorescence derived from functionally expressed GFP involved in the fusion molecule. The surface display of GFP was further verified by immunofluorescence labeling with a polyclonal antibody (raised in rabbits) against GFP as the first antibody and Rhodamine Red-X-conjugated goat anti-rabbit IgG as the second antibody which cannot penetrate into the cell membrane. The display of GFP on the cell surface was confirmed using a confocal laser scanning microscope and by measuring fluorescence in each cell fraction obtained after the subcellular fractionation. As GFP was proved to be displayed as an active form on the cell surface, selection of promoters will endow yeast cells with abilities to respond to changes in environmental conditions, including nutrient concentrations in the media, through the emission of fluorescence.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002539900307
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  • 3
    Electronic Resource
    Electronic Resource
    A new concept for coal water mixture stabilization using a polyelectrolyte (1995)
    Springer
    Colloid & polymer science 273 (1995), S. 490-495 
    Details
    ISSN: 1435-1536
    Keywords: Coal water mixture ; sodium polystyrene sulphonate ; depletion stabilization effect ; concentrated dispersion ; dynamic mobility
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract The stabilization mechanism of Sodium Polystyrene Sulphonate (PSSNa) on coal water mixture (CWM) has been examined using the following colloid chemical concept. It is realized that the stabilization of the CWM is due to an increase in electrostatic repulsion between the coal particles and the electrostatic repulsion is influenced strongly by the concentration of metal cations, especially Ca2+ eluted from the coal surface. The adsorption isotherm of PSSNa on the coal surface indicates a weak affinity type and the desorption amount of PSSNa is tremendously small in compared with the amount of adsorption. This indicates that a lot of PSSNa adsorbed weakly has been eliminated from the surface in the pre-washing process of desorption experiment. Furthermore, it appears that the ζ-potential determined by the Acoustosizer for concentrated coal suspension shows higher values than the values determined by the usual electrophoresis, and that the high values hold for a wide range of salt concentrations in the medium. All these results indicate that much PSSNa is adsorbed weakly on the coal surface and the component plays a role in the stability character of CWM, where a large contribution of depletion stabilization effect can be expected.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00656894
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  • 4
    Electronic Resource
    Electronic Resource
    Effect of sorbitol and inositol on the critical micelle concentration of nonionic surfactants in water and in aqueous urea (1979)
    Springer
    Colloid & polymer science 257 (1979), S. 973-976 
    Details
    ISSN: 1435-1536
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Description / Table of Contents: Zusammenfassung Es wurde die kritische Mizellbildungskonzentration nichtionogener Tenside in Wasser sowie in wäßrigen Harnstofflösungen mit und ohne Zusatz von Inosit und Sorbitol bestimmt. In Wasser wird die cmc bei Zusatz der hexahydrischen Alkohole vermindert; Inosit wirkt dabei stärker als Sorbit. In wässrigen Harnstofflösungen ist der Einfluß auf die cmc und der Unterschied zwischen den zwei Alkoholen geringer als in Wasser. Die Ergebnisse werden über die Beeinflussung der Struktur des Wassers durch die Alkohole gedeutet.
    Notes: Summary The critical micelle concentrations (cmc) of non-ionic surfactants in water and in aqueous urea with or without hexahydric alcohols, sorbitol and inositol, were determined. In water the cmc's of the surfactants were decreased by the addition of the hexahydric alcohols. In addition, there was a remarkable difference in the decreasing ability between these two hexahydric alcohols. Inositol decreased the cmc's more markedly than sorbitol. In aqueous urea the effect of these hexahydric alcohols on the cmc's and the difference in the decreasing ability between the two alcohols were less than those in water. These results were explained in terms of the effect of the hexahydric alcohols on the structure of water.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01520723
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  • 5
    Electronic Resource
    Electronic Resource
    Depressor effect of synthetic peptides related to ACTH on blood pressure in rats (1976)
    Springer
    Cellular and molecular life sciences 32 (1976), S. 368-369 
    Details
    ISSN: 1420-9071
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The depressor effects of natural and synthetic ACTH peptides were demonstrated in the rat. This is an extra-adrenal action of ACTH and is not related to the adrenal-stimulating or melanocyte-stimulating activity of the peptide.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01940843
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  • 6
    Electronic Resource
    Electronic Resource
    Langerhans cells in human allergic contact dermatitis contain varying numbers of birbeck granules (1990)
    Springer
    Histochemistry and cell biology 94 (1990), S. 497-504 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Dynamic changes in human Langerhans cells (LCs) were studied with OK T6, anti-HLA-DR antibody, and Lag antibody in allergic contact dermatitis (ACD). Both T6-positive (T6+) cells and Lag-positive (Lag+) cells in the epidermis decreased in number from 0 to 48 h, but then gradually increased after day 7 of ACD. Lag+ cells after day 7 manifested a variety of staining intensities from weak to strong. It was also shown, after day 7, that some T6+ cells were Lag negative whereas all Lag+ cells were T6 positive. Flow cytometric analysis suggested that Lag-strongly-positive cells and Lag-weakly-positive cells belonged to the same population, and that the relative amount of Lag antigens in T6+ LCs gradually increased after day 7. Immunoelectron microscopy revealed that the Lag-strongly-positive cells contained numerous Lag-reactive Birbeck granules (BGs) whereas the Lag-weakly-positive cells contained fewer BGs in the cytoplasm. In some Lag-weakly-positive cells, no BGs were detected.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00272613
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  • 7
    Electronic Resource
    Electronic Resource
    A novel heterologous gene expression system in Saccharomyces cerevisiae using the isocitrate lyase gene promoter from Candida tropicalis (1996)
    Springer
    Applied microbiology and biotechnology 44 (1996), S. 759-765 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract  We have found that the upstream region of the isocitrate lyase gene (UPR-ICL) from the n-alkane-utilizing yeast Candida tropicalis was functional in Saccharomyces cerevisiae as a novel promoter with non-fermentable carbon sources, such as oleic acid, acetate, ethanol, and glycerol/lactate. The expression of two foreign genes coding for β-galactosidase from Escherichia coli (LacZ) and glutamate decarboxylase from rat brain was carried out under the control of UPR-ICL. Expression of LacZ was repressed by glucose and enhanced over 300-fold by acetate. When an expression vector pWI3 containing multicloning sites between UPR-ICL and the transcriptional terminator of the isocitrate lyase gene (TERM-ICL) was used, the smaller isoform of glutamate decarboxylase (GAD65) was highly produced in a soluble and active form. These results demonstrate that the novel expression system using UPR-ICL and TERM-ICL from C. tropicalis is useful for the production of heterologous proteins in S. cerevisiae.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00178615
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  • 8
    Electronic Resource
    Electronic Resource
    A novel heterologous gene expression system inSaccharomyces cerevisiae using the isocitrate lyase gene promoter fromCandida tropicalis (1996)
    Springer
    Applied microbiology and biotechnology 44 (1996), S. 759-765 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract We have found that the upstream region of the isocitrate lyase gene (UPR-ICL) from then-alkane-utilizing yeastCandida tropicalis was functional inSaccharomyces cerevisiae as a novel promoter with non-fermentable carbon sources, such as oleic acid, acetate, ethanol, and glycerol/lactate. The expression of two foreign genes coding for β-galactosidase fromEscherichia coli (LacZ) and glutamate decarboxylase from rat brain was carried out under the control of UPR-ICL. Expression ofLacZ was repressed by glucose and enhanced over 300-fold by acetate. When an expression vector pW13 containing multicloning sites between UPR-ICL and the transcriptional terminator of the isocitrate lyase gene (TERM-ICL) was used, the smaller isoform of glutamate decarboxylase (GAD65) was highly produced in a soluble and active form. These results demonstrate that the novel expression system using UPR-ICL and TERM-ICL fromC. tropicalis is useful for the production of heterologous proteins inS. cerevisiae.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00178615
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  • 9
    Electronic Resource
    Electronic Resource
    Development of an arming yeast strain for efficient utilization of starch by co-display of sequential amylolytic enzymes on the cell surface (1999)
    Springer
    Applied microbiology and biotechnology 51 (1999), S. 65-70 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract The construction of a whole-cell biocatalyst with its sequential reaction has been performed by the genetic immobilization of two amylolytic enzymes on the yeast cell surface. A recombinant strain of Saccharomyces cerevisiae that displays glucoamylase and α-amylase on its cell surface was constructed and its starch-utilizing ability was evaluated. The gene encoding Rhizopus oryzae glucoamylase, with its own secretion signal peptide, and a truncated fragment of the α-amylase gene from Bacillus stearothermophilus with the prepro secretion signal sequence of the yeast α factor, respectively, were fused with the gene encoding the C-terminal half of the yeast α-agglutinin. The constructed fusion genes were introduced into the different loci of chromosomes of S. cerevisiae and expressed under the control of the glyceraldehyde-3-phosphate dehydrogenase promoter. The glucoamylase and α-amylase activities were not detected in the culture medium, but in the cell pellet fraction. The transformant strain co-displaying glucoamylase and α-amylase could grow faster on starch as the sole carbon source than the transformant strain displaying only glucoamylase.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002530051364
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  • 10
    Electronic Resource
    Electronic Resource
    Preparation and crosslinking of oligoesterified woods based on maleic anhydride and allyl glycidyl ether (1988)
    Springer
    Wood science and technology 22 (1988), S. 21-32 
    Details
    ISSN: 1432-5225
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Summary Oligoesterification of wood was investigated by alternately adding esterification reactions of wood with maleic anhydride and allyl glycidyl ether. The products obtained consisted of acetone-insoluble and soluble parts. The insoluble parts were novel oligoesterified woods with oligoester chains having polymerizable double bonds. The oligoester chain length showed a tendency to decrease with increase in wood content in feed. The soluble parts were free oligoesters which were not linked with the wood matrix. The products (the oligoesterified wood-containing mixtures), when subjected to hot-pressing in the presence of a peroxide, gave plasticized crosslinked wood boards whose surfaces are smooth, glossy, and plasticlike. In this case, the free oligoesters which were hardening worked as a plasticizer for the wood components and were combined, by the crosslinking, with the oligoesterified woods, resulting in the formation of the network structure. The crosslinked wood boards exhibited outstanding properties in heat distortion temperature (〉165°C) and compressive strength (ca. 1,600–2,300 kg/cm2).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00353225
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