ISSN:
1432-072X
Keywords:
Saccharomyces cerevisiae
;
Inhibition of tryptophanyl-tRNA synthetase
;
Mode of action of tryptophan analogues
;
Tryptophan analogue degradation
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract The effect of the tryptophan analogue 4-methyltryptophan in Saccharomyces cerevisiae has been investigated. 4-Methyltryptophan inhibits the aminoacylation of tryptophan specific transfer ribonucleic acid (tRNATrp). The mode of inhibition is competitive and the analogue is not charged onto tRNATrp. Thus 4-methyltryptophan application depletes the cells from charged tRNATrp. As a consequence cell growth and protein synthesis are strongly reduced. 4-Methyltryptophan is degraded efficiently in culture media inoculated with the wild type strain; the effects of 4-methyltryptophan were therefore found to be transient.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00455351
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