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  • Springer  (32)
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  • 1
    Electronic Resource
    Electronic Resource
    Localized axis determinant in the early cleavage embryo of the goldfish, Carassius auratus (1997)
    Springer
    Development genes and evolution 206 (1997), S. 389-396 
    Details
    ISSN: 1432-041X
    Keywords: Key words Dorsoventral axis ; Goldfish ; goosecoid ; no tail ; Zebrafish
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  The teleost dorsoventral axis cannot be distinguished morphologically before gastrulation. In order to examine whether the yolk cell affects axis determination, we bisect early cleavage embryos of the goldfish, Carassius auratus. When the vegetal yolk hemisphere is removed by bisection along the equatorial plane at the 2-cell stage, the embryos develop abnormally and exhibit a symmetrical morphology. No dorsal structures, such as notochord, somites and neural tube, differentiate and no embryonic shield is formed during gastrulation. In addition, no goosecoid mRNA is expressed before gastrulation. The frequency of abnormality decreases as the age at which the vegetal yolk hemisphere is removed increases. Most embryos removed at the 32-cell stage develop normally. Their morphological phenotype is similar to that of a Xenopus ventralized embryo generated by ultraviolet irradiation on the vegetal hemisphere soon after fertilization. We also observed that, when the embryos were bisected along the first cleavage plane at the 2-cell stage, the proportion of pairs of embryos of which one embryo developed normally was 44.8%. These results indicate that the vegetal yolk hemisphere of the early cleavage embryo of the goldfish contains axis determination factor(s), which are necessary for generation of dorsal structures. Furthermore, it is suggested that these determinant(s) are distributed asymmetrically within the vegetal yolk hemisphere.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004270050068
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  • 2
    Electronic Resource
    Electronic Resource
    Morphogenesis of human colon cancer cells with fetal rat mesenchymes in organ culture (1986)
    Springer
    Cellular and molecular life sciences 42 (1986), S. 312-315 
    Details
    ISSN: 1420-9071
    Keywords: Human colon cancer cells ; morphogenesis ; carcinoembryonic antigen ; secretory components ; organ culture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The morphogenesis and cytodifferentiation of human colon cancer cells (LS174T and HT29) were examined by combining cancer cells with fetal rat digestive-tract mesenchyme in organ culture. LS174T cells migrated into the mesenchyme to form glandular structures composed of single columnar cells with their nuclei oriented basally, while HT29 cells formed cell masses with little lumen formation. Immunohistochemical studies with antibodies against carcinoembryonic antigen and secretory components showed that the composition of cell surface glycoproteins was not necessarily reversed to the normal type, even when neoplastic cells exhibited normal glandular structures.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01942518
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  • 3
    Electronic Resource
    Electronic Resource
    HaeIII polymorphism in intron 1 of the human p53 gene (1994)
    Springer
    Human genetics 〈Berlin〉 93 (1994), S. 222-222 
    Details
    ISSN: 1432-1203
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract A new HaeIII polymorphism, which is found in the first intron of the human p53 gene, provides a genetic marker for tumor suppressor p53 gene alterations.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00210619
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  • 4
    Electronic Resource
    Electronic Resource
    Determination of palladium by a new preconcentration method (Mg-W cell)-electrothermal atomic absorption spectrometry (1997)
    Springer
    Fresenius' journal of analytical chemistry 357 (1997), S. 995-997 
    Details
    ISSN: 1432-1130
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract A new concentration method for palladium using Mg-W cell-electrodeposition has been developed. The method was combined with electrothermal atomic absorption spectrometry (ETAAS) with a tungsten tube atomizer. The detection limit of palladium by this method was 0.37 ng ml–1 (3 S/N). The severe interferences on the AAS signal of palladium caused by large amounts of Al, Ca, Cu, Fe, K, Na, Pb and Zn were eliminated by the Mg-W cell-electrodeposition method. The method was adapted for the determination of palladium in environmental samples. The recovery of palladium spiked environmental samples was in the range of 102 to 114%.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002160050289
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  • 5
    Electronic Resource
    Electronic Resource
    Activation of basal transcription by a mutation in SIN4, a yeast global repressor, occurs through a mechanism different from activator-mediated transcriptional enhancement (2000)
    Springer
    Molecular genetics and genomics 263 (2000), S. 48-59 
    Details
    ISSN: 1617-4623
    Keywords: Key words Basal transcription ; Sin4 repression ; Tup1-Ssn6 repression ; Rme1 repression ; Yeast
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The Saccharomyces cerevisiae protein Sin4 has been suggested to affect the transcription of various genes by locally altering chromatin structure. Previous studies have defined two classes of promoters: those which are activated by loss of SIN4 function (termed sin4-responsive promoters) and those which are not activated by sin4 mutations (termed sin4 non-responsive promoters). We analyzed the mechanism of this differential response of the two classes of promoters to a sin4 mutation. The sin4 non-responsive promoters were activated when upstream elements in the promoter region were eliminated. The upstream elements of sin4 non-responsive promoters were, in turn, found to repress the activity of the sin4-responsive promoters in an orientation-independent manner. The sin4-mediated activation was repressed by the Rme1- but not by the Tup1-Ssn6-mediated repression system. Activation of sin4-responsive promoters by Pho4 and the sin4 mutation was additive, and enhancement of transcription driven by sin4-responsive promoters was found to be due to an increase in the basal rate of transcription. The upstream regions in the sin4 non-responsive promoters contained elements that were able to inhibit activation of basal transcription. Based on these observations, we suggest that activation of basal transcription by a mutation in a gene for a global repressor, SIN4, occurs through a mechanism that differs from that responsible for activator-mediated transcriptional enhancement, and we therefore propose that basal transcription and activator-mediated transcription are repressed by different mechanisms.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/PL00008675
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  • 6
    Electronic Resource
    Electronic Resource
    Down regulation of cAMP production by cAMP receptor protein in Escherichia coli: an assessment of the contributions of transcriptional and posttranscriptional control of adenylate cyclase (1996)
    Springer
    Molecular genetics and genomics 253 (1996), S. 198-204 
    Details
    ISSN: 1617-4623
    Keywords: Key words Adenylate cyclase ; cAMP synthesis ; CRP ; Transcriptional regulation ; Posttranscriptional regulation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Escherichia coli cells that are deficient in the cAMP receptor protein (CRP) overproduce cAMP. We and others have previously found that transcription of the adenylate cyclase gene (cya) is negatively regulated by the CRP-cAMP complex. Here, we have investigated the contribution of this transcriptional regulation to the control of cAMP levels. Several variants of the cya gene have been constructed and characterized with respect to their expression and their ability to produce cAMP. Overproduction of cAMP in a crp - background was reduced from 200-fold to 50-fold when transcriptional regulation by CRP-cAMP was eliminated by replacing the cya promoter with the constitutive bla promoter. When the C-terminal 48 amino acids of adenylate cyclase were deleted without changing the promoter, the degree of overproduction of cAMP was reduced to 4-fold. Finally, the increase in cAMP level observed in crp - cells was almost completely abolished when the truncated cyclase was expressed from the bla promoter. We conclude that transcriptional regulation of cya does indeed play a role in the down-regulation of cAMP production by CRP, although the major regulation is exerted at the posttranscriptional level. The C-terminal region comprising the last 48 amino acids of cyclase is responsible for the posttranscriptional regulation. A simple new method for the determination of cAMP is also described.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004380050313
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  • 7
    Electronic Resource
    Electronic Resource
    Isolation of multicopy suppressors of the calcium sensitivity of a mutant lacking the bZIP transcription factor Atf1 in fission yeast (1999)
    Springer
    Molecular genetics and genomics 261 (1999), S. 297-306 
    Details
    ISSN: 1617-4623
    Keywords: Key wordsSchizosaccharomyces pombe ; Signal transduction ; Osmoregulation ; Basic leucine zipper proteins ; MAP kinase cascade
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In Schizosaccharomyces pombe, recent studies have uncovered a set of putative transcription factors of the basic leucine zipper (bZIP) type (e.g., Atf1, Pcr1, Pap1), which function downstream of the Sty1 mitogen-activated protein kinase (MAPK) cascade which is involved in stress-activated signal transduction. Accordingly, a Δatf1 mutant is known to exhibit osmosensitivity for growth, since one of the targets of Atf1 is the gpd1 + gene, which is responsible for the osmoadaptive glycerol production mediated by the Sty1 MAPK cascade. During the course of our studies on the osmotic response in S. pombe, we found that growth of a Δatf1 mutant is highly sensitive to the level of Ca2+ ions in the medium (but less sensitive to Mg2+ and Na+ ions). This phenotype seemed to be relevant to the osmosensitivity, because an Δgpd1 mutant showed a similar phenotype. An attempt was therefore made to isolate multicopy suppressors of the calcium sensitivity exhibited by the Δatf1 cells. Among such suppressors were several bZIP factors, including two known proteins (Atf21 and Pcr1), and two new ones (named Atf31 and Zip1). These factors were characterized further, in comparison to Atf1, with special reference to the Sty1 MAPK signaling pathway.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004380050970
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  • 8
    Electronic Resource
    Electronic Resource
    The Prr1 response regulator is essential for transcription of ste11 + and for sexual development in fission yeast (2000)
    Springer
    Molecular genetics and genomics 264 (2000), S. 441-451 
    Details
    ISSN: 1617-4623
    Keywords: Schizosaccharomyces pombe Phosphorelay Response regulator MAP kinase cascade Meiosis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract. Schizosaccharomyces pombe expresses a putative transcription factor, named Prr1, which is intriguing in the sense that it contains a bacterial type of phospho-accepting receiver domain, preceded by a mammalian heat shock factor (HSF2)-like DNA-binding domain. The receiver domain is most probably involved in an as yet unidentified histidine-to-aspartate (His-to-Asp) phosphorelay pathway in S. pombe. In this study, the structure, function, and cellular localization of Prr1 were assessed in the context of oxidative stress and His-to-Asp phosphorelay. As the most intriguing result of this study, we found that Prr1 is essential not only for the expression of genes induced by oxidative stress (e.g., ctt1 + and trr1 +), but also for the expression of ste11 +, which in turn is responsible for the expression of a variety of genes required for sexual development. Accordingly, Prr1-deficient cells are not only hypersensitive to oxidative stress, but also severely defective in conjugation and/or spore formation. These results suggested that the transcription factor Prr1 plays a pivotal role in an as yet unknown signal transduction pathway that is implicated in sexual differentiation. These findings are discussed with special reference to the well-characterized transcription factors Pap1 and Atf1 of S. pombe.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004380000305
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  • 9
    Electronic Resource
    Electronic Resource
    Changes in molecular species of pepsinogens in the development of the chick (1979)
    Springer
    Cellular and molecular life sciences 35 (1979), S. 814-815 
    Details
    ISSN: 1420-9071
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The potential peptic activity of the chick forestomach increases rapidly at the time of hatching. Together with this, the electrophoretic pattern of the pepsinogens shifts to the adult type. These changes take place even under the complete starvation of a newly hatched chick.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01968268
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  • 10
    Electronic Resource
    Electronic Resource
    Die Einwirkung von Lösungsmitteln auf die Mizellenzerstörung von nichtionogenen oberflächenaktiven Substanzen (1977)
    Springer
    Colloid & polymer science 255 (1977), S. 711-711 
    Details
    ISSN: 1435-1536
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01550121
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