ISSN:
1612-1112
Keywords:
Column liquid chromatography
;
Microwave hydrolysis
;
Microcystin
;
Nodularin
;
D- and L-selective amino acid determination
Source:
Springer Online Journal Archives 1860-2000
Topics:
Chemistry and Pharmacology
Notes:
Summary Microwave radiation has been successfully used for hydrolysis of the hepatotoxic cyclic peptides microcystins and nodularin. Set-up of the microwave device and the operating conditions for microwave hydrolysis were optimized. Results of the microwave hydrolysis were compared with results from conventional hydrolysis for 24 h at 110°C. Microwave hydrolysis of microcystins and nodularin for as little as 10 min at 160°C results in complete cleavage of peptide bonds and high recoveries of amino acids. Enantioselective determination of amino acids was achieved by use of a previously described HPLC method after pre-column derivatization withortho-phthaldialdehyde and the chiral thiolN-iso-butyryl-cysteine.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02466910
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