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  • Springer  (3)
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  • 1
    Electronic Resource
    Electronic Resource
    Use of thin layer chromatography for detection and high performance liquid chromatography for quantitating gliotoxin from rice cultures ofAspergillus fumigatus Fresenius (1989)
    Springer
    Mycopathologia 107 (1989), S. 145-151 
    Details
    ISSN: 1573-0832
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Gliotoxin, a mycotoxin with antimicrobial and immunosuppressive capabilities, is produced by several genera of fungi including the pathogenic fungusAspergillus fumigatus. The ability of selected isolates ofA. fumigatus to produce gliotoxin on three different media was tested and a thin layer chromatographic and high performance liquid chromatographic method for quantitation of gliotoxin from rice culture was developed and is described. Rice cultures were extracted with chloroform and the resulting extract was partially purified by precipitation with petroleum ether and cleanup by gel permeation chromatography. Gliotoxin was detected by thin layer chromatography and quantitated by high performance liquid chromatography using a U.V. absorbance detector with a 254 nm filter and a mobile phase of methanol-water 43∶57 (V/V) with a flow rate of 2.0 ml/min. The retention time for gliotoxin was approximately 4.8 min. From rice samples spiked with gliotoxin concentrations of 0.67, 1.33, 2.67, 4.00 and 5.33μg/g the average recovery was 83.8%.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00707552
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Impact-force source and impact-force calibrator (1973)
    Springer
    Experimental mechanics 13 (1973), S. 257-264 
    Details
    ISSN: 1741-2765
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract A pendulum-force transducer called an impact-force source has been developed which can serve two purposes. The impact-force source can be used to accurately calibrate other force transducers dynamically and can also be used to supply a known and repeatable impact half-sine-type force for impact studies. The magnitude and duration of the impact force produced by the impact-force source can be varied by changing certain physical parameters of the force source. A simple design procedure was developed to determine the values of the parameters of the force source. Experiments were conducted to test the limitations of the impact-force source in its use as a dynamic-force calibrator and as a known source of impact force. The results of these experiments indicate that the force source could be a useful laboratory instrumentation tool.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02322624
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    Paper (German National Licenses)
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  • 3
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    Impact-force source and impact-force calibrator (1973)
    Springer
    Details
    Publication Date: 1973-06-01
    Print ISSN: 0014-4851
    Electronic ISSN: 1741-2765
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Published by Springer
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