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  • Springer  (67)
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  • 1
    ISSN: 1432-1793
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Grass puffer Fugu niphobles, captured in November 1986 at Shimoda, Japan, and then reared at different temperatures ranging from 10 to 29°C, was examined for microflora changes in the skin, gill and intestines. At 10°C, the skin and gill were colonized mainly by Pseudomonas, Flavobacterium and Moraxella, while at 29°C Vibrio and Flavobacterium appeared abundantly. The intestinal microflora, consisting of Vibrio, Pseudomonas and Flavobacterium, revealed little temperature dependence, although the intrageneric composition of Vibrio changed conspicuously depending on the change of water temperature. Vibrio group 1, tentatively identified as V. alginolyticus, was detected in all tissues examined at 20 and 29°C. This, along with the fact that V. alginolyticus produces tetrodotoxin, suggests that the tetrodotoxin contained in puffer is, at least partly, accounted for by this bacterial species inhabiting intestines.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Histochemistry and cell biology 91 (1989), S. 309-314 
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Localization of γ-aminobutyric acid (GABA) in the ventrolateral medulla oblongata of the rat was studied, using antisera directed against GABA molecule fixed to bovine serum albumin. Within the rostral portion of the ventrolateral medulla, GABA-like immunoreactive neurons were found in the lateral wing of the raphe magnus and in the region of the paragigantocellular reticular nucleus. In the caudal portion of the ventrolateral medulla, a lesser number of GABA-stained neurons were found in the region around the nucleus reticularis lateralis. GABA-like immunoreactive punctate structures were also found throughout the ventrolateral medulla. These results provide further evidence for the existence of GABAergic neurons in the ventrolateral medulla oblongata of the rat.
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Molecular genetics and genomics 262 (1999), S. 283-290 
    ISSN: 1617-4623
    Keywords: Key words Sugar beet ; Cytoplasmic male sterility ; Mitochondrial genome ; Genome rearrangement ; Rf gene
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We have constructed a physical map of the mitochondrial DNA of a cytoplasmic male sterile (CMS) sugar beet line, TK81-MS, and compared it with that published for normal fertile sugar beet (cv. TK81-O) to clarify the differences between the CMS and normal mitochondrial genomes. The TK81-MS genome is present as a single circular molecule of 481.8 kb, or as two molecules of 184.9 and 296.9 kb. The CMS genome was found to be highly rearranged relative to the normal mitochondrial genome, with at least fifteen rearrangement and/or inversion events being required to align the two DNAs. Analysis of transcription patterns of known mitochondrial genes and rearranged regions revealed six genes, coxI, coxII, atpA, atp6, rps3, and orf324, whose expression is altered in the CMS line relative to the normal line. Of these six, only the coxI transcript pattern differs between male-sterile and fertility-restored genotypes, making it likely that the coxI locus is involved in mediating CMS in sugar beet.
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Molecular genetics and genomics 262 (1999), S. 822-829 
    ISSN: 1617-4623
    Keywords: Key words Recessive resistance ; Potato virus Y ; Randomly amplified polymorphic DNA (RAPD) ; Nicotiana tabacum L.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Recessive alleles (va, va 1 , va 2 , etc) of the tobacco Va locus confer resistance to potato virus Y (PVY). To elucidate the mechanism underlying this resistance, we attempted to identify randomly amplified polymorphic (RAPD) markers that reveal polymorphism between two nearly isogenic lines (NILs) that differ in their susceptibility to PVY. Using each of 500 primers and 800 pairs of primers, we identified over 100 RAPD fragments that differed between the NILs. We applied these RAPD primers or primer combinations to an F2 population obtained from a cross between the susceptible line BY4 and the resistant va 2 -bearing NIL, F55. It was found that only 10 RAPD markers were polymorphic between resistant and susceptible plants. Unexpectedly, these markers were all linked to Va. All 10 RAPD markers were present in all 8 susceptible varieties tested. At least one RAPD marker was not detected in 8 out of 10 resistant varieties. Southern analysis revealed that the sequences of markers were not present in the genomes of resistant varieties, and the markers were found in individually distinct positions on the chromosomes of susceptible tobacco varieties. These results strongly suggest that the resistance conferred by va is due to deletions at the Va locus governing susceptibility to PVY.
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Computational mechanics 14 (1994), S. 16-27 
    ISSN: 1432-0924
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract The creep behavior of the continuous fiber reinforced unidirectional composites due to the viscoelasticity of the resin matrix is investigated assuming that the constituent matrix obeys the nonlinear creep law and the fiber is the linear elastic materials. Utilizing a quasi three-dimensional finite element method, the macroscopic creep behavior of the composites with regular fiber packing is obtained, giving the orthotropic creep law for the composites. Then, the creep of the composites with random fiber packing is estimated applying the random model proposed by Kondo and Saito in which the neat matrix cylinders are embedded in the regular array composites. The theoretical predictions for the creep behavior are compared with the experimental results.
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Naturwissenschaften 28 (1940), S. 416-416 
    ISSN: 1432-1904
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Natural Sciences in General
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  • 7
    ISSN: 1432-2021
    Keywords: Key words Majorite garnet ; X-ray diffraction ; PVT observation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Geosciences , Physics
    Notes: Abstract The high-pressure and temperature equation of state of majorite solid solution, Mj0.8Py0.2, was determined up to 23 GPa and 773 K with energy-dispersive synchrotron X-ray diffraction at high pressure and high temperature using the single- and double-stage configurations of the multianvil apparatuses, MAX80 and 90. The X-ray diffraction data of the majorite sample were analyzed using the WPPD (whole-powder-pattern decomposition) method to obtain the lattice parameters. A least-squares fitting using the third-order Birch-Murnaghan equation of state yields the isothermal bulk modulus, K T0  = 156 GPa, its pressure derivative, K′ = 4.4(±0.3), and temperature derivative (∂K T /∂T) P = −1.9(±0.3)× 10−2 GPa/K, assuming that the thermal expansion coefficient is similar to that of pyrope-almandine solid solution.
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    Springer
    Physics and chemistry of minerals 26 (1998), S. 1-6 
    ISSN: 1432-2021
    Keywords: Key words high pressure experiment ; San Carlos olivine ; α-β transformation ; transformation mechanisms ; transformation kinetics
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Geosciences , Physics
    Notes: Abstract  Single crystalline San Carlos olivine (1 mm cube) was transformed to (Mg,Fe)2SiO4β-phase at 13.5–15 GPa, 1030–1330 °C for 0–600 min using a multi-anvil high pressure apparatus. The α-β transformation occurred by incoherent surface nucleation and interface-controlled growth and recovered partially transformed samples showed sharply defined reaction rim. The growth rate of the β-phase rim significantly decreased with time and the growth eventually ceased. TEM observations revealed that many dislocations were created in both the relict olivine just near the α-β interface and the β-phase in the rim, which show evidence for deformation caused by interfacial stresses associated with the misfit elastic strain of the transformation. The observed tangled dislocation texture in β-phase suggested that the β-phase rim was hardened and relaxation of the interfacial stress was retarded. This probably caused a localized pressure drop in the relict olivine and decreased the growth rate. Time-dependent growth rates of β-phase is possibly controlled by the rheology of β-phase, which must be considered for the prediction of the olivine metastability in the subducting slabs.
    Type of Medium: Electronic Resource
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  • 9
    Electronic Resource
    Electronic Resource
    Springer
    Naturwissenschaften 27 (1939), S. 278-279 
    ISSN: 1432-1904
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Natural Sciences in General
    Notes: Zusammenfassung Es wurde röntgenographisch beobachtet, daß durch Erhitzungsbehandlung von gereinigten Ramiefasern, sowie der daraus erhaltenen Natrocellulose I mit Glyzerin bei 250° eine ziemlich deutliche Erhöhung des Orientierungsgrades der Kristallite in den nativen Fasern bewirkt wurde. Ein höchstorientiertes Präparat, welches ein besonders scharfes und deutliches Bild mit starker Annäherung an das Drehkristalldiagramm eines Einkristalls zeigt, wurde aus Natroncellulose durch die erwähnte Behandlung erhalten.
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  • 10
    Electronic Resource
    Electronic Resource
    Springer
    Naturwissenschaften 27 (1939), S. 857-858 
    ISSN: 1432-1904
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Natural Sciences in General
    Type of Medium: Electronic Resource
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