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  • Springer  (400)
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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    The journal of membrane biology 168 (1999), S. 131-139 
    ISSN: 1432-1424
    Keywords: Key words: Cell shrinkage — Volume regulation — Patch clamp — Cell lines — Flufenamate — Cation channels
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract. Osmotic cell shrinkage activates a nonselective cation (NSC) channel in M-1 mouse cortical collecting duct cells (Volk, Frömter & Korbmacher, 1995, Proc. Natl. Acad. Sci. USA 92: 8478-8482). To see whether shrinkage-activated NSC channels are an ubiquitous phenomenon, we tested the effect of hypertonic extracellular solution on whole-cell currents of HT29 human colon carcinoma cells, BSC-1 renal epithelial cells, A10 vascular smooth muscle cells, and Neuro-2a neuroblastoma cells. Addition of 100 mm sucrose to an isotonic NaCl bath solution induced cell shrinkage of HT29 cells as evidenced by a decrease in cell diameter from 18 ± 1 μm to 12 ± 1 μm (n= 13). Upon cell shrinkage whole-cell currents of HT29 cells increased within 8 ± 1 min by about 30-fold (n= 13). Cell shrinkage and current activation were reversible upon return to isotonic solution. Replacement of bath Na+ by K+ or Li+ had almost no effect on the stimulated inward current. In contrast, replacement by N-methyl-d-glucamine (NMDG) completely abolished it and shifted the reversal potential from −4.5 ± 0.7 mV to −57 ± 4.1 mV (n= 10). Thus, the stimulated conductance is nonselective for alkali cations but highly selective for cations over anions with a cation-to-anion permeability ratio of about 13. Flufenamic acid (100 μm) inhibited the stimulated current by 84 ± 4.7% (n= 8). During the early phase of hypertonic stimulation single-channel transitions could be detected in whole-cell current recordings, and a gradual activation of 12 and more individual channels with a single-channel conductance of 17.6 ± 0.9 pS (n= 4) could be resolved. In analogous experiments similar shrinkage-activated NSC channels were also observed in BSC-1 renal epithelial cells, A10 vascular smooth muscle cells, and Neuro-2a neuroblastoma cells. These findings indicate that shrinkage-activated NSC channels are an ubiquitous phenomenon and may play a role in volume regulation.
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    The journal of membrane biology 177 (2000), S. 231-242 
    ISSN: 1432-1424
    Keywords: Key words: Cell shrinkage — Volume regulation — Patch clamp — Nonselective cation channels — Cytoskeleton
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract. It has previously been shown that osmotic cell shrinkage activates a nonselective cation (NSC) channel in M-1 mouse cortical collecting duct cells [54] and in a variety of other cell types [20]. In the present study we further characterized the shrinkage-activated NSC channel in M-1 cells and its mechanism of activation using whole-cell current recordings. Osmotic cell shrinkage induced by addition of 100 mm sucrose to the bath solution caused a 20-fold increase in whole-cell inward currents from −10.8 ± 1.5 pA to −211 ± 10.2 pA (n= 103). A similar response was observed when cell shrinkage was elicited using a hypo-osmotic pipette solution. This indicates that cell shrinkage and not extracellular osmolarity per se is the signal for current activation. Cation substitution experiments revealed that the activated channels discriminate poorly between monovalent cations with a selectivity sequence NH4 (1.2) ≥ Na+ (1) ≈ K+ (0.9) ≈ Li+ (0.9). In contrast there was no measurable permeability for Ca2+ or Ba2+ and the cation-to-anion permeability ratio was about 14. The DPC-derivatives flufenamic acid, 4-methyl-DPC and DCDPC were the most effective blockers followed by LOE 908, while amiloride and bumetanide were ineffective. The putative channel activator maitotoxin had no effect. Current activation was dependent upon the presence of intracellular ATP and Mg2+ and was inhibited by staurosporine (1 μm) and calphostin C (1 μm). Moreover, cytochalasin D (10 μm) and taxol (2 μm) reduced the current response to cell shrinkage. These findings suggest that the activation mechanism of the shrinkage-activated NSC channel involves protein kinase mediated phosphorylation steps and cytoskeletal elements.
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  • 3
    ISSN: 1436-2449
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Summary Results of experiments on dark- and photoconductivity of polymeric dibenz[b,i] 1,4,8,11-tetraaza[14]annulenato(μ-pyrazin) iron(II) [Fetaa(pyz)]n are described. By studying the dependence of photoconductivity on electric field, intensity and wavelength it is shown that photogeneration of charge carriers occurred at short wavelengths by an Onsager mechanism and in the near-infrared region by a photoinjection process from the electrodes.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    The European physical journal 228 (1969), S. 322-328 
    ISSN: 1434-601X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract The paper is based on the non-relativistic three quark model of the baryons including mass differences depending on the electromagnetic as well on the hypercharge. Assuming that the binding energies between two quarks in the baryons are the well known linear combinations of the binding energies we verify different mass formulas between the baryons. Some ones can be verified without additional assumptions, other ones only by supposing relations between the binding energies.
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Naturwissenschaften 72 (1985), S. 431-432 
    ISSN: 1432-1904
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Natural Sciences in General
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Naturwissenschaften 75 (1988), S. 360-360 
    ISSN: 1432-1904
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Natural Sciences in General
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  • 7
    ISSN: 1432-1904
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Natural Sciences in General
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    Springer
    Naturwissenschaften 38 (1951), S. 333-333 
    ISSN: 1432-1904
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Natural Sciences in General
    Type of Medium: Electronic Resource
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  • 9
    Electronic Resource
    Electronic Resource
    Springer
    Naturwissenschaften 70 (1983), S. 252-254 
    ISSN: 1432-1904
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Natural Sciences in General
    Type of Medium: Electronic Resource
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  • 10
    Electronic Resource
    Electronic Resource
    Springer
    The European physical journal 181 (1964), S. 301-316 
    ISSN: 1434-601X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract Directional correlation measurements have been made of six different gamma-gamma-cascades in Cd110, following theβ-decay of Ag110m. The observed correlation functions are: 764 keV−1505 keV-cascade: W(ϑ)=1−(0,1578±0,0075)P 2−(0,0075±0,0065)P 4 1505 keV−658 keV-cascade: W(ϑ)=1−(0,395±0,013)P 2−(0,032±0,012)P 4 1384 keV−884 keV-cascade: W(ϑ)=1−(0,2840±0,0110)P 2−(0,0137±0,0090)P 4 1384 keV−658 keV-cascade: W(ϑ)=1−(0,3013±0,0065)P 2−(0,0130±0,0055)P 4 937 keV−884 keV-cascade: W(ϑ)=1+(0,098±0,012)P 2+(0,0148±0,0058)P 4 884 keV−658 keV-cascade: W(ϑ)=1+(0,1004±0,0087)P 2+(0,0086±0,0046)P 4 Together with recent experimental results on internal conversion coefficients, these measurements permit to make unique spin and parity assigments to all known levels but one of Cd110.
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