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  • Springer  (349)
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  • 1
    Electronic Resource
    Electronic Resource
    A bacterial extracellular polysaccharide which enhances the attachment, ofAgrobacterium tumefaciens to the plant cell surface (1989)
    Springer
    Cellular and molecular life sciences 45 (1989), S. 201-202 
    Details
    ISSN: 1420-9071
    Keywords: Agrobacterium tumefaciens ; agglutination ; extracellular polysaccharide ; Xanthobacter sp. ; cell attachment
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Intensive screening, of soil microbial cultures for agglutinating activity ofAgrobacterium tumefaciens resulted in the discovery of a bacterium,Xanthobacter sp. KB-3001, which produced an agglutinin ofA. tumefaciens. This compound is an acidic polysaccharide consisting of glucose and galacturonic acid in the ratio 4∶1. This compound assists the attachment ofA. tumefaciens to plant cells and promotes crown gall formation, owing to its affinity to bothA. tumefaciens and plant cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01954877
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  • 2
    Electronic Resource
    Electronic Resource
    Well-defined genome architecture in the human sperm nucleus (1995)
    Springer
    Chromosoma 103 (1995), S. 577-590 
    Details
    ISSN: 1432-0886
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract. Using fluorescence in situ hybridization, conventional epifluorescence microscopy, and laser scanning confocal microscopy followed by three-dimensional reconstruction we describe a well-defined higher order packaging of the human genome in the sperm cell nucleus. This was determined by the spatial localization of centromere and telomere regions of all chromosomes and supported by localization of subtelomere sequences of chromosome 3 and the entire chromosome 2. The nuclear architecture in the human sperm is characterized by the clustering of the 23 centromeres into a compact chromocenter positioned well inside the nucleus. The ends of the chromosomes are exposed to the nuclear periphery where both the subtelomere and the telomere sequences of the chromosome arms are joined into dimers. Thus chromosomes in the human sperm nucleus are looped into a hairpin-like configuration. The biological implications of this nuclear architecture in spermatogenesis and male pronuclear formation following fertilization are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00357684
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  • 3
    Electronic Resource
    Electronic Resource
    de Haas-van Alphen oscillations and angular magnetoresistance oscillations in the organic metal κ-(BETS)2GaCl4 (1999)
    Springer
    Journal of experimental and theoretical physics 88 (1999), S. 114-117 
    Details
    ISSN: 1090-6509
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract Quantum de Haas-van Alphen oscillations and semiclassical angular magnetoresistance oscillations are observed in the quasi-two-dimensional organic metal κ-(BETS)2GaCl4. The behavior of these oscillations attests to the existence of two cylindrical Fermi surface sheets with axes perpendicular to the conducting plane. The cross-sectional areas of these cylinders in the conducting plane are equal to about 20 and 100% of the corresponding cross section of the Brillouin zone. It is shown that the many-particle interaction can be suppressed significantly in this compound.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1134/1.558772
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  • 4
    Electronic Resource
    Electronic Resource
    Determination of mechanical properties of impacted human morsellized cancellous allografts for revision joint arthroplasty (1999)
    Springer
    Journal of materials science 10 (1999), S. 755-760 
    Details
    ISSN: 1573-4838
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine , Technology
    Notes: Abstract This paper deals with the characterization of mechanical properties of impacted morsellized cancellous allograft (IMCA) produced by dynamic compaction of allograft femoral heads ground by commercially available bone mills, i.e. rotating rasp and reciprocating type bone mills. Various ranges and profiles of particle size in the graft aggregates were obtained using these bone mills, and the effect of number of compaction as well as the distribution of particle sizes on the mechanical properties of IMCA under quasistatic compression and shear loading conditions was discussed. The morsellized cancellous allograft prepared by the reciprocating type bone mill showed a broad distribution of particle sizes, and gave IMCA superior mechanical properties to the graft with a more uniform size distribution, or prepared by the rotating rasp type bone mills. The increase of number of compaction also improved the mechanical properties of IMCA in compression.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1008995630200
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  • 5
    Electronic Resource
    Electronic Resource
    Effect of particles and interface conditions on fibrous tissue interposition between bone and implant. A particle challenge model in rabbit (2000)
    Springer
    Journal of materials science 11 (2000), S. 255-259 
    Details
    ISSN: 1573-4838
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine , Technology
    Notes: Abstract Interposed fibrous tissue at bone–implant interfaces was quantitatively measured in the presence or absence of polyethylene (PE) or alumina particles. Three different conditions of the interface were designed by implanting a pre-polymerized polymethylmethacrylate (PMMA) plug (plug group), a doughy PMMA (injection group) and a hydroxyapatite (HA) plug (HA group) in the hole drilled at the intercondylar notch of rabbit knees. PE (170±18 μm) or alumina particles (88±26 μm) were repeatedly administered into the knee joints at one month intervals (six times). All animals were sacrificed seven months after the implantation. The bone–implant interface was histomorphometrically examined using undecalcified ground sections. In the plug group, the PE particles significantly increased the extent of the interposed fibrous tissue (p 〈 0.05), while the alumina particles showed no effect. In contrast, both particles showed no significant effects in the injection and the HA groups. These results indicate that both particle characteristics and conditions of the bone–implant interface affected particle-induced fibrous tissue interposition. The loose PMMA plug with PE particles induced the greatest amount of fibrous tissue interposition. ©2000 Kluwer Academic Publishers
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1008936830622
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  • 6
    Electronic Resource
    Electronic Resource
    Histological evaluation of bone—cement interface affected by polyethylene particles in rabbit knee (1994)
    Springer
    Journal of materials science 5 (1994), S. 610-612 
    Details
    ISSN: 1573-4838
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine , Technology
    Notes: To investigate the biological process of aseptic component loosening caused by polyethylene wear debris, nine rabbits were implanted with acrylic cement into the non-weight-bearing intercondylar notch of distal femur. Six animals were administrated the particles of polyethylene into the knee joint repeatedly for 12 weeks. At the bone-cement interface, thin connective tissue was observed, while bone sometimes existed directly next to the acrylic cement. The percentage of the length of interposed fibrous tissue against the total length of bone-cement interface was measured. The percentage was 15.8±10.3 in the polyethylene-injected group and 8.3±7.7 in the control group (no significance). While not significant, the amount was greater in the polyethylene-injected group. Thus it is proposed that the polyethylene particles played a role in bone resorption and fibrous tissue formation at the bone—cement interface. In some specimens, macrophages and foreign body giant cells that surrounded the particles near the articular surface were seen to cause resorption of bone. It is supposed that this phenomenon is similar to the focal osteolysis that is sometimes observed around a prosthetic component.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00120340
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  • 7
    Electronic Resource
    Electronic Resource
    Modulation of the phenotypic and functional properties of phagocytic macrophages by wear particles from orthopaedic implants (1997)
    Springer
    Journal of materials science 8 (1997), S. 641-648 
    Details
    ISSN: 1573-4838
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine , Technology
    Notes: Abstract An attempt was made to assess the local chronic inflammatory response in patients with failed orthopaedic implant that is clinically associated with osteolysis, bone and bone marrow necrosis. The main objective was to analyse the heterogeneity of the macrophage functional subsets in the bone–implant interface membrane and to evaluate their possible role in the development of an erosive inflammatory lesion within the bone. Immunohistology was performed on 21 specimens of the bone–implant interface obtained from 17 patients during revision arthroplasty, and synovial membranes from rheumatoid (RA, n=4), and osteoarthritis (OA, n=4) patients. Three well-characterized monoclonal antibodies (MAb) recognizing antigenic determinants on specific functional subsets of macrophages (Mφ) were used. RFD1 (interdigitating reticulum cells/antigen presenting cells, (APC), RFD7 (mature phagocytic macrophages), and RFD9 epithelioid cells and foreign body giant cells (FBGC). RFD1 was expressed on a variable number of perivascular and synovial lining Mφ in both RA and OA synovia, at a frequency of 25%–40%. In cases with total joint replacements, the interface showed a marked increase in the expression of RFD1 (20%–90%). A considerably greater percentage of RFD1 positive Mφ and FBGC was noted in the interfaces from cases with a high level of detectable metal particulate wear debris (mean 80%, range 60%–90%) than in cases with polyethylene wear debris (mean 30%, range 0%–50%), p 0.0001. RFD7 labelled most tissue Mφ in each group. Immunoreactivity for RFD9 was restricted to FBGC in all cases analysed. The finding of elevated expression of RFD1 on metal-containing Mφ and FBGC in the bone-implant interface suggests an increase in antigen-presenting phenotype and indicates that metal particles have more impact in the induction of immune-mediated responses. Such responses are characterized by sustained cellular hyperreactivity and phenotypic changes in Mφ subsets.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1018575504518
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  • 8
    Electronic Resource
    Electronic Resource
    Well-defined genome architecture in the human sperm nucleus (1995)
    Springer
    Chromosoma 103 (1995), S. 577-590 
    Details
    ISSN: 1432-0886
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Using fluorescence in situ hybridization, conventional epifluorescence microscopy, and laser scanning confocal microscopy followed by three-dimensional reconstruction we describe a well-defined higher order packaging of the human genome in the sperm cell nucleus. This was determined by the spatial localization of centromere and telomere regions of all chromosomes and supported by localization of subtelomere sequences of chromosome 3 and the entire chromosome 2. The nuclear architecture in the human sperm is characterized by the clustering of the 23 centromeres into a compact chromocenter positioned well inside the nucleus. The ends of the chromosomes are exposed to the nuclear periphery where both the subtelomere and the telomere sequences of the chromosome arms are joined into dimers. Thus chromosomes in the human sperm nucleus are looped into a hairpin-like configuration. The biological implications of this nuclear architecture in spermatogenesis and male pronuclear formation following fertilization are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00357684
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  • 9
    Electronic Resource
    Electronic Resource
    Pharbitis class-1 knotted-like homeobox gene, PKn3, shares similar characteristics to those of class-2 knotted-like genes (2000)
    Springer
    Plant cell reports 19 (2000), S. 911-920 
    Details
    ISSN: 1432-203X
    Keywords: Key words Homeobox ; Knotted ; Pharbitis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Three novel homeobox genes, PKn1–3 (Pharbitis knotted-like), were isolated from Japanese morning glory (Pharbitis nil Chois, strain Violet). A sequence analysis showed that these genes belong to the knotted class-1 gene family and that PKn3 has a relatively unique sequence. All PKn genes are expressed in shoot apices, stems and roots, but not in cotyledons. Transformed tobacco with PKn1 or PKn2 displayed leaf shrinkage and a dwarf phenotype, while the ectopic expression of PKn3 gave no altered phenotypes. In situ hybridization showed that PKn3 is up-regulated in developing leaf primordia and that this expression becomes restricted in the basal region of young leaf blades, which is reminiscent of the expression pattern of the class-2 knotted gene, NTH23. These data suggest that these Pharbitis homeobox genes participate in the differentiation in shoots and suggest a unique function of PKn3 in developing leaves.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002990000214
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  • 10
    Electronic Resource
    Electronic Resource
    Interplay between chains of localised spins and two-dimensional sheets of organic donors in the synthetically built magnetic multilayer (1998)
    Springer
    The European physical journal 1 (1998), S. 439-452 
    Details
    ISSN: 1434-6036
    Keywords: PACS. 71.30.+h Metal-insulator transitions and other electronic transitions - 75.30.Kz Magnetic phase boundaries (including magnetic transitions metamagnetion, etc.) - 75.30.Fv Spin density waves
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract: In order to understand the magnetic field-induced restoration of a highly conductive state in , static (SQUID) and dynamic (ESR and AFR) magnetization measurements were performed on polycrystalline samples and single crystals, respectively. In addition, cantilever and resistivity measurements under steady fields were performed. While the metal-insulator transition curve of the ( ) phase diagram exhibits a first order character, a “spin-flop” transition line divides the insulating state when the magnetic field is applied along the easy axis of magnetization. The effects of a RKKY-type indirect exchange and of applied magnetic field are described within the framework of a generalized Kondo lattice, namely two chains of localised spins coupled through the itinerant spins of the 2D sheets of BETS. The calculations, which can incorporate intramolecular electron correlations within a mean field theory, are in qualitative agreement with the field induced transition from the antiferromagnetic insulating ground state to a canted one, i.e. a not fully oriented paramagnetic, but metallic state.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s100510050207
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