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1

Digitale Medien

Vascular expression of the grp1.8 promoter is controlled by three specific regulatory elements and one unspecific activating sequence (1994)

Keller, Beat ; Heierli, Daniel

Springer

Plant molecular biology 26 (1994), S. 747-756

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ISSN: 1573-5028

Schlagwort(e): activating sequence ; gene expression ; glycine-rich protein ; tobacco ; vascular expression

Quelle: Springer Online Journal Archives 1860-2000

Thema: Biologie

Notizen: Abstract The bean grp1.8 full-length promoter is specifically active in vascular tissue during normal development of tobacco. Deletion of a negative regulatory element resulted in ectopic activity of the promoter in cortical cells of hypocotyls, roots and stems. A 169 bp fragment (−205 to −36) of the grp1.8 promoter conferred vascular-specific expression to CaMV 35S minimal promoters whereas a 141 bp fragment (−205 to −64) strongly activated these minimal promoters both in vascular and cortical cells. These experiments defined a new regulatory element (VSE) that is essential for vascular-specific expression and is located between −64 and −36. The 141 bp grp1.8 promoter sequence had enhancer-like properties as it was active in both orientations. A 24 bp sequence (bp −119 to −96, corresponding to the SE1 regulatory element) enhanced expression from several minimal promoters strongly but unspecifically, whereas a 26 bp sequence (−98 to −73, corresponding to the RSE regulatory element) induced vascular-specific expression. Thus, the grp1.8 promoter is regulated by a combinatorial mechanism that can integrate the action of different, non-additively acting regulatory elements into vascular-specific expression.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1007/BF00013759

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2

Digitale Medien

Common occurrence of homologues of petunia glycine-rich protein-1 among plants (1996)

Cheng, Shu-Hua ; Keller, Beat ; Condit, Carol M.

Springer

Plant molecular biology 31 (1996), S. 163-168

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ISSN: 1573-5028

Schlagwort(e): petunia ; glycine-rich protein ; monocot ; dicot

Quelle: Springer Online Journal Archives 1860-2000

Thema: Biologie

Notizen: Abstract The presence of specific glycine-rich proteins (GRP) related to petunia GRP1 (ptGRP1) was examined in three species of monocots (wheat, barley and maize) and five species of dicots (rape, turnip, soybean, crabapple and tomato). Protein blot analysis showed that anti-ptGRP1 antibody cross-reacted with a single different polypeptide in all species except maize. The molecular mass of these polypeptides ranged from 14 to 55 kDa. Tissue-print immunoblots of rape petioles and stems showed that the rape ptGRP1 homologue, like ptGRP1, is primarily located in the vascular tissue, and that its expression decreases with developmental age of the tissue. In barley, the ptGRP1 homologue is found in leaf vascular bundles, and may also be present in the surrounding bundle sheaths. Unlike the dicots examined, expression of the protein did not appear to decrease significantly with developmental age.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1007/BF00020616

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3

Digitale Medien

Genetic and physical characterization of theLR1 leaf rust resistance locus in wheat (Triticum aestivum L.) (1995)

Feuillet, Catherine ; Messmer, Monika ; Schachermayr, Gabriele ; [weitere]

Springer

Molecular genetics and genomics 248 (1995), S. 553-562

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ISSN: 1617-4623

Schlagwort(e): Leaf rust ; RFLP ; Sequence-tagged-site (STS) ; Wheat ; Resistance gene

Quelle: Springer Online Journal Archives 1860-2000

Thema: Biologie

Notizen: Abstract The objective of this study was to characterize the leaf rust resistance locusLr1 in wheat. Restriction fragment length polymorphism (RELP) analysis was performed on the resistant lineLr1/6*Thatcher and the susceptible varieties Thatcher and Frisal, as well as on the segregating F2 populations. Seventeen out of 37 RFLP probes mapping to group 5 chromosomes showed polymorphism betweenLr1/6*Thatcher and Frisal, whereas 11 probes were polymorphic between the near-isogenic lines (NILs)Lr1/6*Thatcher and Thatcher. Three of these probes were linked to the resistance gene in the segregating F2 populations. One probe (pTAG621) showed very tight linkage toLr1 and mapped to a single-copy region on chromosome 5D. The map location of pTAG621 at the end of the long arm of chromosome 5D was confirmed by the absence of the band in the nulli-tetrasomic line N5DT5B of Chinese Spring and a set of deletion lines of Chinese Spring lacking the distal part of 5DL. Twenty-seven breeding lines containing theLr1 resistance gene in different genetic backgrounds showed the same band asLr1/6*Thatcher when hybridized with pTAG621. The RFLP marker was converted to a sequence-tagged-site marker using polymerase chain reaction (PCR) amplification. Sequencing of the specific fragment amplified from both NILs revealed point mutations as well as small insertion/deletion events. These were used to design primers that allowed amplification of a specific product only from the resistant lineLr1/6*Thatcher. This STS, specific for theLr1 resistance gene, will allow efficient selection for the disease resistance gene in wheat breeding programmes. In addition, the identification of a D-genome-specific probe tightly linked toLr1 should ultimately provide the basis for positional cloning of the gene.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1007/BF02423451

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4

Digitale Medien

Specific interaction of the tomato bZIP transcription factor VSF-1 with a non-palindromic DNA sequence that controls vascular gene expression (1998)

Ringli, Christoph ; Keller, Beat

Springer

Plant molecular biology 37 (1998), S. 977-988

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ISSN: 1573-5028

Schlagwort(e): bZIP transcription factor ; cell wall ; glycine-rich protein ; vascular-specific expression ; xylem

Quelle: Springer Online Journal Archives 1860-2000

Thema: Biologie

Notizen: Abstract The grp1.8 gene of French bean (Phaseolus vulgaris) is specifically expressed in vascular tissue and encodes a glycine-rich structural protein (GRP1.8) of the cell wall. Earlier promoter analysis had shown that a 28 bp fragment of the grp1.8 promoter (vs-1) confers vascular expression to heterologous minimal promoters and is bound by the tomato bZIP transcription factor VSF-1. Here, we analysed the interaction of VSF-1 with fragments of the vs-1 element and studied the molecular basis of specific binding both in the DNA sequence of the promoter element as well as in the protein. The minimal binding site of VSF-1 is a 9 bp, non-palindromic sequence with two non-identical half-sites and a central nucleotide which separates them. The amino acid sequence of the VSF-1 DNA-binding basic domain has a Lys at position -10 instead of a conserved Arg found in the other bZIP factors isolated so far. This lysine was found to be required for specific recognition of the non-palindromic binding site: a mutant VSF-1 with a Lys-to-Arg substitution at position -10 bound with higher affinity to a palindromic sequence than the wild-type protein. The minimal binding site of VSF-1 was sufficient and necessary to confer vascular-specific expression to a heterologous promoter in vivo. The vsf-1 promoter also showed vascular-specific expression in transgenic tobacco. The close similarity of these expression patterns suggests that VSF-1 is specifically involved in vascular expression of the grp1.8 gene in plants.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1023/A:1006030007333

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5

Digitale Medien

Molecular characterization of a new type of receptor-like kinase (wlrk) gene family in wheat (1998)

Feuillet, Catherine ; Reuzeau, Christophe ; Kjellbom, Per ; [weitere]

Springer

Plant molecular biology 37 (1998), S. 943-953

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ISSN: 1573-5028

Schlagwort(e): barley ; kinase ; leaf rust ; receptor-like kinase ; resistance ; wheat

Quelle: Springer Online Journal Archives 1860-2000

Thema: Biologie

Notizen: Abstract In plants, several types of receptor-like kinases (RLK) have been isolated and characterized based on the sequence of their extracellular domains. Some of these RLKs have been demonstrated to be involved in plant development or in the reaction to environmental signals. Here, we describe a RLK gene family in wheat (wlrk, wheat leaf rust kinase) with a new type of extracellular domain. A member of this new gene family has previously been shown to cosegregate with the leaf rust resistance gene Lr10. The diversity of the wlrk gene family was studied by cloning the extracellular domain of different members of the family. Sequence comparisons demonstrated that the extracellular domain consists of three very conserved regions interrupted by three variable regions. Linkage analysis indicated that the wlrk genes are specifically located on chromosome group 1 in wheat and on the corresponding chromosomes of other members of the Triticeae family. The wlrk genes are constitutively expressed in the aerial parts of the plant whereas no expression was detected in roots. Protein immunoblots demonstrated that the WLRK protein coded by the Lrk10 gene is an intrinsic plasma membrane protein. This is consistent with the hypothesis that WLRK proteins are receptor protein kinases localized to the cell surface. In addition, we present preliminary evidence that other disease resistance loci in wheat contain genes which are related to wlrk.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1023/A:1006062016593

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6

Digitale Medien

Molecular markers for the detection of the wheat leaf rust resistance gene Lr10 in diverse genetic backgrounds (1997)

Schachermayr, Gabriele ; Feuillet, Catherine ; Keller, Beat

Springer

Molecular breeding 3 (1997), S. 65-74

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ISSN: 1572-9788

Schlagwort(e): leaf rust ; molecular marker ; receptor-like kinase ; resistance breeding ; resistance gene ; wheat

Quelle: Springer Online Journal Archives 1860-2000

Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft

Notizen: Abstract We recently showed that the Lr10 wheat leaf rust resistance gene cosegregated with the candidate resistance gene Lrk10 which encodes a putative receptor-like kinase. The aim of this study was to develop Lrk10-derived molecular markers for the detection of the Lr10 gene in breeding material. Different subfragments of Lrk10 were tested as RFLP markers for the Lr10 resistance gene. The most specific fragment (Lrk10-6) was converted into the PCR-based STS marker STSLrk10-6. Both the RFLP and the STS marker did not give a signal with near isogenic lines containing a different Lr gene. The applicability of these markers for the detection of Lr10 in genetically diverse material was tested with 62 wheat and spelt breeding lines, mostly from European breeding programmes. Twelve varieties known to have Lr10 showed the same alleles as the originally characterized line ThatcherLr10. Most of the lines with unknown composition at the Lr10 locus had a null allele with both the RFLP marker Lrk10-6 and the marker STSLrk10-6 whereas 20% of the lines had a different allele. For six lines, including a traditional spelt variety derived from a landrace, both markers showed the same allele as Thatcher Lr10. Artificial infections of these lines with an isolate avirulent on Lr10 resulted in a hypersensitive reaction of all these lines, indicating also the presence of the Lr10 resistance gene. These data demonstrate that the markers derived from sequences of Lrk10 are highly specific for the Lr10 gene in breeding material of very diverse genetic origin. The markers will allow the defined deployment of Lr10 in wheat breeding programmes and will contribute to the elucidation of the role of Lr10 in polygenic resistances against leaf rust.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1023/A:1009619905909

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