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  • 1
    Electronic Resource
    Electronic Resource
    Selective removal of N-terminal methionine from recombinant human growth hormone by an aminopeptidase isolated from Aspergillus flavus (1998)
    Springer
    Journal of industrial microbiology and biotechnology 20 (1998), S. 287-290 
    Details
    ISSN: 1476-5535
    Keywords: Keywords: aminopeptidase; N-terminal methionine; recombinant protein; recombinant human growth hormone
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: An aminopeptidase was isolated from a soil fungus, which specifically cleaves the unnatural N-terminal methionine in recombinant human growth hormone. Reaction mixtures with different ratios of aminopeptidase to recombinant methionyl human growth hormone showed that the removal of N-terminal methionine was complete at 1:200 (w/w), and more than 90% complete at ratios up to 1:2000 (w/w) when incubated for 24 h at 37°C. The data indicate that the aminopeptidase we have purified can be used for the efficient conversion of unnatural recombinant proteins to their natural form.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/sj.jim.2900525
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  • 2
    Electronic Resource
    Electronic Resource
    Further characterization of the effects of ultraviolet irradiation of the amphibian egg (1978)
    Springer
    Cellular and molecular life sciences 34 (1978), S. 883-884 
    Details
    ISSN: 1420-9071
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary UV-irradiation of the vegetal hemisphere of amphibian eggs leads to developmental abnormalities in neural morphogenesis. The possibility that the egg's transient sensitivity to irradiation could be due to pigmentation changes was examined in albino eggs. The tissue specificity of the effects of irradiation was analyzed by exchanging the ectoderm between irradiated and control embryos.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01939682
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  • 3
    Electronic Resource
    Electronic Resource
    An error estimate in the EFG method (1998)
    Springer
    Computational mechanics 21 (1998), S. 91-100 
    Details
    ISSN: 1432-0924
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract In this paper, local and global error estimates for the element-free Galerkin (EFG) method are proposed. The essence of proposed error estimates is to use the difference between the values of the projected stress and these given directly by the EFG solution. The stress projection can be obtained simply by taking product of shape function based on a different domain of influence with the stresses at nodes. In this study, it was found that the effectivity index is optimized if the domain of influence in stress projection procedure is the smallest that retains regularity of the matrices in EFG. Numerical tests are shown for various 1D and 2D examples illustrating the good effectiveness of the proposed error estimator in the global energy norm and in the local error estimates.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004660050286
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  • 4
    Electronic Resource
    Electronic Resource
    An event-based, robot-served flexible manufacturing cell modeller (1993)
    Springer
    The international journal of advanced manufacturing technology 8 (1993), S. 102-110 
    Details
    ISSN: 1433-3015
    Keywords: Robot programming ; Flexible manufacturing cell ; FMC ; Simulation modelling ; Machine utilisation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract The development is described of a flexible manufacturing cell (FMC) programming and simulation system called FMCPS, which generates robot programs for a 6-DOF industrial robot. The development of FMCPS is based on an FMC that is being developed at UMIST. The cell consists of a Fanuc 600 ARCmate industrial robot, a Takisawa MAC V3 CNC machining centre and an MHP MT-50 CNC turning centre. The software has been developed on a SUN 4/110 workstation running under the UNIX operating system, using three-dimensional SUNCORE interactive graphics and SUNVIEW window systems. An event-oriented robot-programming method is used in the FMCPS. This method divides the robot tasks into different events. The user needs to define the points of the robot gripper route and the corresponding data for each event. The Cartesian coordinates of the points in the robot gripper paths are then transformed to the six robot joint angles by a variant inverse kinematics iteration method. These robot joint angles are used in the simulation model which generates robot programs suitable for the Fanuc robot. A three-dimensional, wire-frame-based graphical model is used for simulation. The user can observe the simultaneous animation of the robot movement, and display machine tool paths in separate graphics windows on the screen simultaneously. The various activities in the cell are simulated in pseudo-parallel mode by taking advantage of the multi-tasking and piping features of UNIX. At the end of a simulation exercise, the user is provided with the facility to obtain a performance report for the FMC which gives information on total operation time and on robot and machine utilisation, in addition to the robot program.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01748775
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  • 5
    Electronic Resource
    Electronic Resource
    Three-photon contributions in optically pumped lasers (1980)
    Springer
    International journal of infrared and millimeter waves 1 (1980), S. 27-44 
    Details
    ISSN: 1572-9559
    Keywords: three-photon interactions ; density matrix ; far infrared lasers ; signal flow graphs
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract A signal flow graph representation of the density matrix equations appropriate to a serial three-wave interaction is introduced, from which perturbation theory and AC Stark shift information may be extracted. The conditions for a large interaction cross section for the three-photon transition are deduced and applied to selected experimental situations with the main conclusion being that the effect may be present in a large number of CW and pulsed experiments.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01007064
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  • 6
    Electronic Resource
    Electronic Resource
    Preparation and electrochemical characterization of LiCoO2 by the emulsion drying method (2000)
    Springer
    Journal of applied electrochemistry 30 (2000), S. 1081-1085 
    Details
    ISSN: 1572-8838
    Keywords: emulsion drying ; LiCoO2
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Electrical Engineering, Measurement and Control Technology
    Notes: Abstract Fine powders of LiCoO2 were successfully synthesized through an emulsion drying method. The oxide powders were characterized by thermogravimetric-differential thermal analyses, X-ray diffractometry, scanning electron microscopy, elemental analysis and electrochemical method including charge–discharge cycling. By post-annealing the powders obtained by the emulsion drying method in the temperature range 600 ∼ 900 ∘C, we obtained LiCoO2 powder which has a layer structure (R3¯m) and consists of fine particles with submicrometre order in diameter. The charge–discharge characteristics as a cathode for lithium ion battery depended on the post-annealing temperature. LiCoO2 powder made by the emulsion drying method displays a good electrochemical performance, suggesting that this soft chemistry approach has the potential to synthesize a high quality electrode material.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1004042604318
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  • 7
    Electronic Resource
    Electronic Resource
    Microstructure–Ionic Conductivity Relationships in Perovskite Lithium Lanthanum Titanate (1999)
    Springer
    Journal of materials science 18 (1999), S. 493-496 
    Details
    ISSN: 1573-4811
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1006606817633
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  • 8
    Electronic Resource
    Electronic Resource
    Frequency and distance of transposition of a modifiedDissociation element in transgenic tobacco (1996)
    Springer
    Transgenic research 5 (1996), S. 343-357 
    Details
    ISSN: 1573-9368
    Keywords: heterologous transposon tagging ; Ac/Ds system ; visual transposition assays ; position effects ; Nicotiana tabacum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Effective transposon tagging with theAc/Ds system in heterologous plant species relies on the accomplishment of a potentially high transposon-induced mutation frequency. The primary parameters that determine the mutation frequency include the transposition frequency and the transposition distance. In addition, the development of a generally applicable transposon tagging strategy requires predictable transposition behaviour. We systematically analysedDs transposition frequencies andDs transposition distances in tobacco. An artificialDs element was engineered with reporter genes that allowed transposon excision and integration to be monitored visually. To analyse the variability ofDs transposition between different tobacco lines, eight single copy T-DNA transformants were selected. Fortrans-activation of theDs elements, differentAc lines were used carrying an unmodifiedAc + element, an immobilizedsAc element and a stableAc element under the control of a heterologous chalcone synthas (chsA) promoter. With allAc elements, eachDs line showed characteristic and heritable variegation patterns at the seedling level. SimilarDs line-specificity was observed for the frequency by whichDs transpositions were germinally transmitted, as well as for the distances of theDs transpositions. ThesAc element induced transposition ofDs late in plant development, resulting in low germinal transposition frequencies (0.37%) and high incidences of independent transposition (83%). The majority of theseDs elements (58%) transposed to genetically closed linked sites (≤10 cM).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01968944
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  • 9
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    A pilot plant study on reclamation of sewage effluent by radiation treatment (2004)
    Springer
    Details
    Publication Date: 2004-01-01
    Print ISSN: 0236-5731
    Electronic ISSN: 1588-2780
    Topics: Chemistry and Pharmacology , Energy, Environment Protection, Nuclear Power Engineering
    Published by Springer
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  • 10
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    On the Use of Outer Approximations as an External Active Set Strategy (2010)
    Springer
    Details
    Publication Date: 2010-02-12
    Print ISSN: 0022-3239
    Electronic ISSN: 1573-2878
    Topics: Mathematics
    Published by Springer
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