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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Journal of materials science 7 (1996), S. 413-414 
    ISSN: 1573-4838
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine , Technology
    Notes: Abstract Enzyme superoxide dismutase (SOD) incorporation parameters were evaluated after immobilization in polyisobutylcyanoaclylate (PIBCA) nanoparticles. After initialization of the anionic mechanism of polymerization, pH was increased and its effect on the characteristics of PIBCA nanoparticles analysed. Our goal included optimization of enzyme activity during incorporation into nanoparticles and the influence on size distribution. Unloaded nanoparticles were not significantly affected by the pH increase. At pH 3 the size distribution indicates a bimodal distribution: 58 nm (63%) and 146 nm (37%). When pH was increased to 5 after 1 h of polymerization the size distribution is: 57 nm (70%) and 125 nm (30%). When pH was increased to 5, after 2 h of polymerization, the size distribution is 67 nm (56%) and 160 nm (44%). Meanwhile, the retention of activity of SOD in polymerization medium is 49% at pH 3, and 98% at pH 5. The effect of pH increase from 3 to 5, after 1 h of polymerization, on the characteristics of loaded nanoparticles is an increase of retention of enzyme activity (18 to 30%); and the evidence of a pH-dependent smaller size population of loaded nanoparticles. In fact at pH 3 the size distribution is 83 nm (15%), 195 nm (15%), 440 nm (70%) and when pH is increased from 3 to 5 the size distribution becomes 55 nm (30%); 170 nm (30%); 430 nm (40%).
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Journal of structural chemistry 3 (1962), S. 299-310 
    ISSN: 1573-8779
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Theoretical chemistry accounts 72 (1987), S. 393-401 
    ISSN: 1432-2234
    Keywords: Local density approximation ; Xα method ; Ab initio SCF parametersα ; Ab initio statistical parametersα ; Electron binding energies ; Transition state method
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract Local exchange-correlation potential has been derived starting from the free electron gas model.Ab initio way of calculating the parameterα of the Xα method is presented. Self-consistent and statistical exchange-correlation parameters have been determined. The self-consistent parametersα have been used to calculate the electron binding energies of Neon, Argon and Krypton. We suggest using statistical exchange-correlation parameter in molecular calculations. The statistical exchange-correlation parameter has been applied to study the electron binding energies of the molecules H2O and HF. It is shown that the electron binding energies calculated with the self-consistent and the statistical parametersα show agreement with the experimental values.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Boundary layer meteorology 13 (1978), S. 61-85 
    ISSN: 1573-1472
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences , Physics
    Notes: Abstract This paper reviews analytical methods in electromagnetic scattering theory (i.e., geometrical and physical optics, perturbation, iteration, and integral-equation) which are applicable to the problems of remote sensing of the ocean. In dealing with Earth's surface (in this case, the weakly non-linear ocean), it is not possible to have a complete and exact description of its spatial and temporal statistics. Only the first few moments are generally available; and in the linear approximation the statistics are assumed homogeneous, stationary and Gaussian. For this case, the high-frequency methods (geometrical and physical optics) and perturbation (Rayleigh-Rice), or a combination of them, provide tractable analytical results (i.e., the specular-point, the slightly-rough Bragg scattering and the composite-surface models). The applicability and limitations of these models are discussed. At grazing incidence and for higher frequencies, other scattering mechanisms become significant; and shadowing, diffraction and trapping must be considered. The more exact methods (integral-equation and Green's function) have not been as successful in yielding tractable analytical solutions, although they have the potential to provide improved theoretical scattering results in the future.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Bioscience reports 19 (1999), S. 601-609 
    ISSN: 1573-4935
    Keywords: Cationic liposomes ; luciferase ; H9 cells ; PM1 cells ; GALA
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Transgene expression in lymphoid cells may be useful for modulating immune responses in, and gene therapy of, cancer and AIDS. Although cationic liposome-DNA complexes (lipoplexes) present advantages over viral vectors, they have low transfection efficiency, unfavorable features for intravenous administration, and lack of target cell specificity. The use of a targeting ligand (transferrin), or an endosome-disrupting peptide, in ternary complexes with liposomes and a luciferase plasmid, significantly promoted transgene expression in several T- and B-lymphocytic cell lines. The highest levels of luciferase activity were obtained at a lipid/DNA (±) charge ratio of 1/1, where the ternary complexes were net negatively charged. The use of such negatively charged ternary complexes may alleviate some of the drawbacks of highly positively charged plain lipoplexes for gene delivery.
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Magnetic resonance materials in physics, biology and medicine 2 (1994), S. 421-423 
    ISSN: 1352-8661
    Keywords: phosphorus metabolites ; 31P relaxation ; relaxation mechanisms ; chemical shift anisotropy ; spin rotation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine , Physics
    Notes: Abstract Measurements have been made of the longitudinal relaxation timeT 1 of31P for the individual resonances of the metabolites AMP, ADP, ATP, Pir and PCr (phosphocreatine) in H2O and D2O solutions from 5 to 60°C at various concentrations and at frequencies of 40 MHz (2.3 T) and 120 MHz (7 T). The contributions of dipolar, chemical shift anisotropy, and spin-rotation mechanisms have been separated, and activation parameters of the underlying molecular reorientations have been determined.
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  • 7
    Publication Date: 1978-01-01
    Print ISSN: 0006-8314
    Electronic ISSN: 1573-1472
    Topics: Geosciences , Physics
    Published by Springer
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  • 8
    Publication Date: 1994-10-01
    Print ISSN: 0968-5243
    Electronic ISSN: 1352-8661
    Topics: Medicine , Physics
    Published by Springer
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