ISSN:
1432-0614
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Process Engineering, Biotechnology, Nutrition Technology
Notes:
Abstract Polycyclic aromatic hydrocarbons (PAH) are persistent priority pollutants of soil and sediments. The use of white-rot fungi has been proposed as a means of bioremediating PAH-polluted sites. However, higher PAH compounds of low bioavailability in polluted soil are biodegraded slowly. In order to enhance their bioavailability, PAH solubilization, can be increased in water/solvent mixtures. The oxidation of a model PAH compound, anthracene, in the presence of cosolvents by the white-rot fungus, Bjerkandera sp. strain BOS55 was investigated. Acetone and ethanol at 5% were toxic to this fungus when added at the time of inoculation. However, when solvents up to 20% (v/v) were added to 9-day-old cultures, ligninolytic activity as indicated by Poly R-478 dye decolorization and anthracene oxidation was evident for several days. Since 20% solvent was toxic to cells, the oxidation of anthracene can be attributed to extracellular peroxidases, which were shown to tolerate the solvent. Solvent additions of 11%–21% (v/v) acetone or ethanol increased the rate of anthracene bioconversion to anthraquinone in liquid medium by a factor of 2–3 compared to fungal cultures receiving 1%–3% solvent.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00164508
Permalink