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  • Springer  (271)
  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Cellular and molecular life sciences 38 (1982), S. 367-369 
    ISSN: 1420-9071
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Reproducing females ofOncopeltus fasciatus which were treated with exogenous makisterone A and 20-hydroxyecdysone laid eggs with considerably elevated ecdysteroid contents. The early embryonic development was markedly accelerated when the mother was treated with makisterone A, whereas 20-hydroxyecdysone had no influence.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1432-1807
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mathematics
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Naturwissenschaften 1 (1913), S. 353-355 
    ISSN: 1432-1904
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Natural Sciences in General
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1432-1424
    Keywords: Key words: Intestinal peptide transporter — Expression — Substrate specificity — Two-electrode voltage-clamp technique
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract. The cloned intestinal peptide transporter is capable of electrogenic H+-coupled cotransport of neutral di- and tripeptides and selected peptide mimetics. Since the mechanism by which PepT1 transports substrates that carry a net negative or positive charge at neutral pH is poorly understood, we determined in Xenopus oocytes expressing PepT1 the characteristics of transport of differently charged glycylpeptides. Transport function of PepT1 was assessed by flux studies employing a radiolabeled dipeptide and by the two-electrode voltage-clamp-technique. Our studies show, that the transporter is capable of translocating all substrates by an electrogenic process that follows Michaelis Menten kinetics. Whereas the apparent K0.5 value of a zwitterionic substrate is only moderately affected by alterations in pH or membrane potential, K0.5 values of charged substrates are strongly dependent on both, pH and membrane potential. Whereas the affinity of the anionic dipeptide increased dramatically by lowering the pH, a cationic substrate shows only a weak affinity for PepT1 at all pH values (5.5–8.0). The driving force for uptake is provided mainly by the inside negative transmembrane electrical potential. In addition, affinity for proton interaction with PepT1 was found to depend on membrane potential and proton binding subsequently affects the substrate affinity. Furthermore, our studies suggest, that uptake of the zwitterionic form of a charged substrate contributes to overall transport and that consequently the stoichiometry of the flux-coupling ratios for peptide: H+/H3O+ cotransport may vary depending on pH.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Archives of microbiology 99 (1974), S. 61-70 
    ISSN: 1432-072X
    Keywords: BranchedOrtho-Cleavage-Pathway ; 3- and 5-Chloro-3,5-cyclohexadiene·1,2-diol-1-carboxylic Acid
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A pseudomonad has been isolated from sewage, which can utilize 3-chlorobenzoic acid as a sole carbon source. In cells grown on benzoate the enzymes of the β-ketoadipic acid pathway are present. Considerable enzymic activities for chlorinated substrates were found in benzoate grown cells only for the oxygenation of 3-chlorobenzoate and the dehydrogenation of 3- and 5-chloro-3,5-cyclohexadiene-1,2-diol-1-carboxylic acid. 3-Chlorobenzoate grown cells show additional high activities for the turnover of 3- and 4-chlorocatechols and chloromuconic acids.
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Archives of microbiology 21 (1955), S. 310-328 
    ISSN: 1432-072X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    Archives of microbiology 119 (1978), S. 7-11 
    ISSN: 1432-072X
    Keywords: Clostridium formicoaceticum ; Fumarate reductase ; Peripheral enzyme ; Membrane protein
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract When Clostridium formicoaceticum was grown on fumarate or l-malate crude cell extracts contained a high fumarate reductase activity. Using reduced methyl viologen as electron donor the specific activity amounted to 2–3.5 U per mg of protein. Reduced benzyl viologen, FMNH2 and NADH could also serve as electron donors but the specific activities were much lower. The NADH-dependent activity was strictly membrane-bound and rather labile. Its specific activity did not exceed 0.08 U per mg of particle protein. Fumarate reductase activity was also found in cells of C. formicoaceticum grown on fructose, gluconate, glutamate and some other substrates. The methyl viologen-dependent fumarate reductase activity could almost completely be measured with intact cells whereas only about 25% of the cytoplasmic acetate kinase activity was detected with cell suspensions. The preparation of spheroplasts from cells of C. formicoaceticum in 20 mM HEPES-KOH buffer containing 0.6 M sucrose and 1 mM dithioerythritol resulted in the specific release of 88% of the fumarate reductase activity into the spheroplast medium. Only small amounts of the cytoplasmic proteins malic enzyme and acetate kinase were released during this procedure. These results indicate a peripheral location of the fumarate reductase of C. formicoaceticum on the membrane.
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  • 8
    Electronic Resource
    Electronic Resource
    Springer
    Archives of microbiology 10 (1939), S. 6-12 
    ISSN: 1432-072X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Type of Medium: Electronic Resource
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  • 9
    Electronic Resource
    Electronic Resource
    Springer
    Applied physics 43 (1987), S. 167-172 
    ISSN: 1432-0649
    Keywords: 33.50 ; 78.65
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract Fluorescence lifetimes of Scheibe-aggregates of Pseudoisocyanine chloride (PIC) in aqueous solution and of the PIC derivative 1-methyl-1′-octa-decyl-2,2′-cyanine perchlorate in stacks of molecular monolayers produced on glass substrates using the Langmuir-Blodgett technique have been measured. The decays are non-exponential and lifetimes depent on sample composition. Fluorescence lifetimes of the short-lived component in assemblies of 40 dye monolayers show a non-normal temperature dependence: τ293K=8.2 ps and τ143K=5.5 ps.
    Type of Medium: Electronic Resource
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  • 10
    ISSN: 1432-1017
    Keywords: Key words Diacetylenic lipids ; Polymerization ; Microfluorescence ; Filmbalance ; AFM
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Physics
    Notes: Abstract Molecular films from polymeric materials play an important role in basic research as well as in technology. We have synthesized the double chain diacetylenic ammonium lipid N-bis-(10, 12-pentacosadiinoyl)-oxy-ethyl)-N-methyl-amine (ONCO). We have characterized monomolecular films at the air/water interface by means of microfluorescence filmbalance techniques and by atomic force microscopy (AFM). ONCO forms stable monomolecular films that exhibit a fluid-solid phase transition with a transition enthalpy of 90 kJ/mol at 10 °C and neutral pH. The coexistence pressure was found to decrease with decreasing protonation, which is in contrast to the commonly found Coulomb mechanism. A change in the chain packing due to a different nitrogen bond angle is discussed as a possible mechanism. This model is cor-roborated by the finding that crystals at high and at low pH differ in their polymerization properties as measured by microfluorescence.
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