ISSN:
1573-5079
Keywords:
ferredoxin
;
ferredoxin: NADP reductase
;
chemical cross-linking
;
protein-protein interactions
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Ferredoxin and the flavoprotein, ferredoxin: NADP reductase, have been covalently linked by incubation in the presence of a water soluble carbodiimide. The cross-linking reaction yields an adduct having a 1:1 stoichiometry. The adduct has depressed levels of diaphorase and NADPH oxidase activity and is inactive in reduction of cytochrome c using NADPH as an electron donor. Thus, although similar to an adduct described by Zanetti and coworkers [J Biol Chem 259: 6153–6157 (1984)] in its stoichiometry, the adduct described herein has significantly different enzymatic properties. It is suggested that this may be a reflection of differences in the interaction between the two proteins resulting from differences in experimental conditions in which the two adducts were prepared.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00035450
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