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  • 1
    Electronic Resource
    Electronic Resource
    IgG, IgG1 and IgM response in Trichinella spiralis-infected mice treated with 4-deoxypirydoxine or fed a Vitamin B6-deficient diet (1999)
    Springer
    Molecular and cellular biochemistry 194 (1999), S. 47-52 
    Details
    ISSN: 1573-4919
    Keywords: Vitamin B6-deficiency ; infected mice ; 4-deoxypirydoxine
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract The aim of this study was to investigate the effect of pyridoxine (Vitamine B6) deficiency on the immunological response of BALB/c mice infected with the parasite T. spiralis. Specific anti-parasite IgM and IgG immunoglobulins were detected by ELISA method in the serum of treated animals at different periods for 60 days post infection. Vitamin B6-deficiency was induced in two separate groups of mice by either (1) maintaining the mice on a Vitamin B6-deficient synthetic pellet diet for 40 days before infection, or (2) by daily intraperitoneal injection of 8 ×105 M/100 μg of 4-Deoxypyridoxine (4-DPD), a potent antagonist of Vitamin B6 for 20 days prior to infection. These two groups of mice were then injected with 100 larvae (L1-T. spiralis) per os. Parasite burdens in the mice were observed by light microscopy. Cysts were present in the diaphragms of the mice after 60 days post-infection. Parasite specific IgG, as well as IgG. levels were determined in the sera of infected mice fed a normal diet. These levels were found to be lower in the 4-DPD-treated mice compared to the untreated mice. The inhibition started from the 10th day and continued to the 60th day, and in the 4-DPD treated group the inhibition initiated after 24 h to 60 days. IgM level also was depressed by 4-DPD, starting from 24 h after injection of the compound. In mice fed Vitamin B6-deficient diets the levels of IgG were lower than in mice fed normal diets. These results show that BALB/c mice infected with T. spiralis and fed either a Vitamin B6-deficient diet or a diet which included the Vitamin B6-antagonist, 4-DPD, both influence the course of IgG, IgGI and IgM production.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1006996603605
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  • 2
    Electronic Resource
    Electronic Resource
    Monocyte Chemotactic Protein-1 Gene Expression and Translation in Formed Granulomatous Calcified Tissue In Vivo (1999)
    Springer
    Calcified tissue international 64 (1999), S. 57-62 
    Details
    ISSN: 1432-0827
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Abstract. Monocyte chemotactic protein-1 (MCP-1) and related molecules constitute the C-C class of the β chemokine supergene family with inflammatory properties. However, the exact role, function, and implication in inflammatory diseases remain to be determined. Here we report that subcutaneous injections (0.2 ml) of a saturated water solution (1:40) of potassium permanganate crystals induces the generation of granuloma tissue at the site of injection in the rat, and reaches its peak of formation after 1 week. The size and weight of the granulomas were increased by i.p. lipopolysaccharide (LPS) (6 μg/200 μl) and inhibited by intraperitoneal (i.p.) dexamethasone (Dxs) 300 μg/200 μl) treatments in rats, injected 18 hours before sacrifice. Moreover, steady-state levels of MCP-1 mRNA in the granuloma tissue (control), were strongly generated. Rats treated i.p. with LPS produced an increase of MCP-1 mRNA in the granuloma tissue compared with controls (i.p. PBS-treated) whereas in animals treated with Dxs, there was a decrease in (P 〈 0.05) in formation of mRNA protein. When the granuloma tissues were homogenized the generation of MCP-1 was found in the supernatants. The level of MCP-1 was higher (P 〈 0.05) in the LPS-treated animals and lower (P 〈 0.05) in the Dxs group compared with the controls (treated with PBS). Similar results were obtained in the serum and in minced granuloma tissue where samples were further incubated in vitro with LPS (100 ng/ml) overnight. A Strong increase (P 〈 0.01) in MCP-1 in all samples was detected, but not in the minced granuloma tissue from Dxs-treated animals. Our data demonstrate that calcified tissue from chronic inflammation induced by KMnO4 generates MCP-1 gene expression and translation, an effect increased by LPS and decreased by Dxs.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002239900579
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  • 3
    Electronic Resource
    Electronic Resource
    Calcium content of experimental granuloma as determined by X-ray fluorescence. Influence of non-steroid anti-inflammatory drugs (1980)
    Springer
    Cellular and molecular life sciences 36 (1980), S. 230-231 
    Details
    ISSN: 1420-9071
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The deposit of calcium in experimental granulomas as determined by X-ray fluorescence is notably decreased by orally given indomethacin and diflunisal.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01953749
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  • 4
    Electronic Resource
    Electronic Resource
    In situ X-ray absorption spectroelectrochemical study of hydroxocobalamin (2000)
    Springer
    JBIC 5 (2000), S. 156-166 
    Details
    ISSN: 1432-1327
    Keywords: Key words X-ray absorption spectroscopy ; Hydroxocobalamin ; Spectroelectrochemistry ; Oxidation state
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract  An in situ X-ray absorption spectroscopy (XAS) spectroelectrochemical study of aquocobalamin (system B12a-B12r-B12s) has been carried out in aqueous solutions buffered at different pH values. To the best of our knowledge, this is the first structural study of aquocobalamin at room temperature under controlled oxidation conditions. Most of the previous work was in fact performed using frozen samples chemically treated to produce the species. The spectroelectrochemical approach offers several advantages: (1) the reduction products may be studied without poisoning the system with chemical reductive reagents and (2) any possible variation of the oxidation state owing to the electrons produced by the incident beam is avoided as the electrode, under potentiostatic control, acts as a scavenger. The spectroelectrochemical approach, together with more careful data analysis, has led to an improved interpretation of the XAS data. These conditions were not met in previous works where the oxidation state was not controlled and multiple scattering contributions were not taken into account. The general shape of the XAS spectra of the different species is not greatly affected by pH. A signature for the base-off square-planar coordination has been evidenced for the Co(II) compound at basic pH. A new signature for Co(I), indicating square-planar coordination, has been identified on the experimental spectra and simulated in theoretical X-ray absorption near-edge structure (XANES) studies. The flexibility of the electrochemical approach, that permits to unambiguously establish the formal oxidation state, has led to very reliable values for energy shift and peak intensity variations. The experimental XANES and extended X-ray absorption fine structure (EXAFS) spectra with a very good signal-to-noise ratio have been processed using the GNXAS package that takes into account multiple scattering contributions. EXAFS and XANES independent analysis result in the same structural model. The reduction from Co(III) to Co(II) produces the most significant structural changes: the cobalt coordination number decreases from six to five, and the edge position shifts by 2.4±0.3 eV. In addition, the XANES spectra are strongly modified. The reduction from Co(II) to Co(I) produces mainly electronic effects with no apparent change of the coordination number. A discussion of the limits and potentialities of EXAFS in this type of study has also been included.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s007750050360
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  • 5
    Electronic Resource
    Electronic Resource
    Induction of monocyte chemotactic protein-1 (MCP-1) and TNF alpha by Trichinella spiralis in serum of mice in vivo (1998)
    Springer
    Molecular and cellular biochemistry 179 (1998), S. 1-5 
    Details
    ISSN: 1573-4919
    Keywords: monocyte chemotactic protein-1 (MCP-1) ; tumor necrosis factor ; Trichinella spiralis ; infection
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract MCP-1 is a small (8-10 KDa) protein and a prototype member of the CC chemokine β subfamily, which plays a critical role in acute and chronic inflammation. Recent evidence suggests an important role for MCP-1, MCP-2 and MCP-3 in a number of pathological states, including delayed type hypersensitivity conditions, parasitic infections and rheumatoid arthritis. Forty BALB-c mice were treated with the parasite Trichinella spiralis. After the infection the animals were sacrificed at different periods from the initial infection and MCP-1 and TNFα were quantified in the mouse serum. The level of MCP-1 in the serum of mice infected with 100 larvae increase 27.5 ± 7.0 pg/ml at day 23, to a maximum level of 31.5 ± 5.0 pg/ml at day 33, from then decreased to 14.6 ± 2.0 pg/ml at day 47. When the mice were infected with 200 larvae of T. spiralis the maximum increase was 34.4 ± 2.5 pg/ml found on day 23. From day 33 to day 47 MCP-1 levels were decreased. In addition, in infected mice levels of TNFα were detectable in the serum as early as day 1. The level of TNFα was maximum at day 35 (3812 ± 224 pg/ml). Serum from non-infected mice contained no detectable levels of either MCP-1 or TNFα. However, even if MCP-1 seems to be implicated in Trichinellosis, its exact role and function in inflammatory parasitic diseases remains to be determined.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1006875429323
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  • 6
    Electronic Resource
    Electronic Resource
    Vitamin B6-deficient diet plus 4-deoxypyridoxine (4-DPD) reduces the inflammatory response induced by T. spiralis in diaphragm, masseter and heart muscle tissue of mice (1999)
    Springer
    Molecular and cellular biochemistry 197 (1999), S. 79-85 
    Details
    ISSN: 1573-4919
    Keywords: vitamin B6 ; Trichinella spiralis ; 4-deoxypyridoxine (4-DPD) ; inflammation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract Animals fed diets deficient in vitamin B6 develop microcytic anemia, alterations of growth, and other pathologies. 4-deoxypirydoxine is a potent antagonist of vitamin B6 coenzyme which depresses IL-1, TNF and IL-6 and has anti-inflammatory properties. The aim of this study was to show the anti-infl ammatory effects of 4-DPD on chronic inflammation caused by the nematode parasite T. spiralis, specifically on the recruitment and the activation of inflammatory cells. Two groups of mice, 6 weeks of age, were used: one was maintained on a vitamin B6-deficient synthetic pellet diet for 15 days before injection of the nematode, and administered an intraperitoneal injection (i.p.) of 4-DPD (250 μg/mouse) for 15 days (the first, 5 days before infection), and the second group was maintained on a normal diet for the total duration of the experiment. These two groups were then injected with 150 larvae (L1-T. spiralis) per os. Chronic inflammation was caused by infection of treated or untreated mice with T. spiralis parasite. After 14 days post-infection all mice developed a chronic inflammatory response. Mice fed with a B6-deficient diet showed a significant decrease in the number of cysts found in the diaphragm when compared to mice treated with normal diet. In addition, in all mice treated with vitamin B6-deficient diet plus 4-DPD the average body weight was significantly lower, compared to the mice on normal diet in all weeks examined. Moreover, in sections of the diaphragm, masseter and miocardium muscles, the infiltration of inflammatory cells, such as macrophages, lymphocytes, and eosinophils were more intense in untreated mice compared to those fed a vitamin B6-deficient diet. These results show that BALB/c mice infected with T. spiralis and fed a vitamin B6-deficient diet plus the vitamin B6 antagonist, 4-DPD, prolong the time of invasion of the larvae in the muscle cells, influence the recruitment of inflammatory cells and the intensity of the inflammatory reaction compared to infected untreated mice (control)
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1006958310081
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  • 7
    Electronic Resource
    Electronic Resource
    Human recombinant interleukin-1β induces thromboxane A2 release in polymorphonuclear leukocytes, macrophages and platelets: Effect of IL-1 receptor antagonist (1996)
    Springer
    Molecular and cellular biochemistry 159 (1996), S. 163-168 
    Details
    ISSN: 1573-4919
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract Prostaglandins and thromboxanes (T×s) are produced by polymorphonuclears (PMNs) and macrophages (MØs) in response to various stimuli. PMNs were separated from other human blood cells and MOs were separated from rat peritoneal lavage. In this paper we show that human recombinant interleukin-1 (hrIL-1) can stimulate the release of thromboxane B2(T×B2) by PMNs and MØs. In addition, we have shown that aggregation of PMNs may occur when calcium ions (7 mM) and hrIL-1 (100 ng/ml) are added to the cell preparation, but not when Ca2+ alone, hrIL-1 alone, or first hrIL-1 then calcium are added to the cell preparation. The treatment of human platelets with hrIL-1 shows that after 15 min incubation T×B2 is released. In addition, we compared the aggregation of platelets caused by ADP with that caused by hrIL-1. Human recombinant IL-1 at a concentration of 100 ng/ml also causes little aggregation of platelets, in this case the aggregation is reversible. In conclusion, hrIL-1β stimulates T×B2 release in PMNs, MØs and platelets and this effect increases with addition of Ca2+ ions. The mixture of hrIL-1 and Ca2+ causes little aggregation of PMNs. In monocyte suspensions, pretreated with human recombinant IL-1 receptor antagonist (IL-Ira) 500 ng/ml for 10 min and then treated with LPS or hrIL-1β 10 μg/ml, the release of T×B2 was partially inhibited. IL-Ira may play a significant role in the control of IL-1 and LPS induction in the release of T×B2.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00420919
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  • 8
    Electronic Resource
    Electronic Resource
    Limiting distributions of randomly accelerated motions (1997)
    Springer
    Lithuanian mathematical journal 37 (1997), S. 219-229 
    Details
    ISSN: 1573-8825
    Keywords: order statistics ; Central Limit Theorem ; uniform acceleration ; Poisson process
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mathematics
    Notes: Abstract In this paper, the process {X(t); t〉0}, representing the position of a uniformly accelerated particle (with Poisson-paced) changes of its acceleration, is studied. It is shown that the distribution ofX(t) (suitably normalized), conditionally on the numbern of changes of acceleration, tends in distribution to a normal variate asn goes to infinity. The asymptotic normality of the unconditional distribution ofX(t) for large values oft is also shown. The study of these limiting distributions is motivated by the difficulty of evaluating exactly the conditional and unconditional probability laws ofX(t). In fact, the results obtained in this paper permit us to give useful approximations of the probability distributions of the position of the particle.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02465352
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  • 9
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    Calcium content of experimental granuloma as determined by X-ray fluorescence. Influence of non-steroid anti-inflammatory drugs (1980)
    Springer
    Details
    Publication Date: 1980-02-01
    Print ISSN: 0014-4754
    Topics: Biology , Medicine
    Published by Springer
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  • 10
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    Human recombinant interleukin-1β induces thromboxane A2 release in polymorphonuclear leukocytes, macrophages and platelets: Effect of IL-1 receptor antagonist (1996)
    Springer
    Details
    Publication Date: 1996-09-01
    Print ISSN: 0300-8177
    Electronic ISSN: 1573-4919
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Published by Springer
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