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  • 1
    ISSN: 1432-072X
    Keywords: Endoglucanase ; Exoglucanase ; Cellulomonas fimi ; Divergent promoter ; Broad-host range
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A cartridge was constructed which contained the divergent tet promoters of transposon Tn10 between an exoglucanase gene (cex) and an endoglucanase gene (cenA) of Cellulomonas fimi. When carried in a broad-host-range vector, the cartridge gave expression of cex and cenA in Escherichia coli, Rhodobacter capsulatus and Klebsiella pneumoniae.
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Monatshefte für Chemie 103 (1972), S. 482-491 
    ISSN: 1434-4475
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Description / Table of Contents: Zusammenfassung In Au-diffundierten, Phosphor-dotierten Si-Scheiben wurden durch Transmissionselektronenmikroskopie feine Ausscheidungsteilchen beobachtet, von denen angenommen wurde, daß sie aus einer Au−P-Verbindung bestehen. Um die für eine Identifizierung dieser Ausscheidung mittels Elektronenbeugung notwendigen Daten der Kristallstruktur zu eihalten, wurde eine geringe Menge von Au2P3-Einkristallen durch eine Gasphasentransportreaktion der Elemente hergestellt. Die erhaltenen Kristalle wurden chemisch und durch Röntgenbeugung untersucht. Weiters wurden Aussagen über die Morphologie und die Stabilität der Au2P3-Kristalle erhalten.
    Notes: Abstract Fine precipitate particles assumed to consist of a Au−P compound were observed by transmission electron microscopy in Au-diffused phosphorus-doped Si-wafers. To obtain crystal structure data required for an electron diffraction identification of this precipitate, a small amount of Au2P3 single crystals was grown from the elements by a vapor transport method and analyzed by chemical and X-ray diffraction methods. In addition, information on the morphology and stability of Au2P3 crystals was obtained.
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Calcified tissue international 9 (1972), S. 122-130 
    ISSN: 1432-0827
    Keywords: Tetracycline ; Development ; Calcification ; Statolith ; Nematocysts ; Aurelia
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Description / Table of Contents: Résumé L'effet de la tétracycline HCl sur la synthèse de statolithes de sulfate de calcium chezAurelia a été étudié. La tétracycline inhibe la synthèse de statolithes et nématocystes à un stade précoce de strobilation. La tétracycline, cependant, n'est pas incorporée dans les statolithes ou nématocystes en formation. Comme la tétracycline ne se combine pas avec le calcium des statolithes de sulfate de calcium dihydraté d'Aurelia, l'explication des effets d'inhibition sur la différenciation de statolithes et nématocystes ne semble pas liée avec un facteur en rapport avec l'incorporation. Des étudesin vitro de quatre systèmes inorganiques de calcium et de tétracycline montrent que le sulfate de calcium dihydraté (gypse) n'incorpore pas la tétracycline: il en est de même de son équivalent isostructural, le phosphate de calcium hydrogéné dihydraté (brushite). Le carbonate de calcium et le phosphate de calcium (apatite) incorpore la tétracycline. L'explication des différences de comportement du calcium peut être liée à la structure cristalline des composés respectifs, et, en particulier, au fait que l'ion Ca est prêt ou non à réagir avec la tétracycline.
    Abstract: Zusammenfassung Es wird über die Wirkung von Tetracyclinchlorhydrat auf die Synthese von Calciumsulfat-Statolithen beiAurelia berichtet. Wird das Tetracyclin in einem Frühstadium der Strobilation verabreicht, so hemmt es die Synthese der Statolithen und der Nematocysten. Das Tetracyclin wird jedoch nicht in die sich bildenden Statolithen oder Nematocysten eingebaut. Da sich das Tetracyclin nicht mit dem Calcium der Calciumsulfatdihydrat-Statolithen derAurelia verbindet, so kann dessen Hemmwirkung auf die Statolithen und die sich differenzierenden Nematocysten offenbar nicht mit einem einbaubedingten Faktor erklärt werden. Untersuchunge, die in vitro mit vier verschiedenen anorganischen Calciumsalzen und Tetracyclin ausgeführt wurden, zeigten, daß weder Calciumsulfatdihydrat (Gips), noch dessen isotrukturelles Aequivalent Calciumhydrogenphosphatdihydrat (Bruschit) Tetracyclin einbauen. Dagegen inkorporieren Calciumcarbonat und Calciumphosphat (Apatit) das Tetracyclin. Die Erklärung für dieses unterschiedliche Verhalten der Calciumsalze findet sich in der Kristallstruktur der betreffenden Verbindungen, d.h. es hängt davon ab, ob das Calciumion ür die Reaktion mit Tetracyclin leicht verfügbar ist.
    Notes: Abstract The effect of tetracycline HCl on synthesis of calcium sulphate statoliths inAurelia is reported. Tetracycline inhibits synthesis of statoliths and nematocysts when administered at an early stage of strobilation. The tetracycline, however, is not incorporated into the developing statoliths or nematocysts. As the tetracycline does not combine with the calcium of the calcium sulfate dihydrate statoliths ofAurelia, an explanation for its inhibitory effects on statoliths and nematocyst differentiation apparently does not rest with an incorporation-related factor. In vitro studies of four inorganic calcium systems and tetracycline revealed that calcium sulfate dihydrate (gypsum) did not incorporate tetracycline nor did its isostructural equivalent, calcium hydrogen phosphate dihydrate (brushite). Calcium carbonate and calcium phosphate (apatite) did incorporate tetracycline. The explanation for these different behaviors of calcium can be found in the crystal structure of the respective compounds, namely, whether or not the Ca ion is readily available to react with tetracycline.
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  • 4
    ISSN: 1617-4623
    Keywords: Key words Cell wall proteins ; Chlamydomonas reinhardtii ; Gamete-specific genes ; Gene expression patterns ; Hydroxyproline-rich glycoproteins
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Gametic differentiation in Chlamydomonas reinhardtii is a two-step process, which is controlled by the sequential action of the two extrinsic signals, nitrogen starvation and blue light. The gamete-specific genes GAS28 and GAS29 are expressed in the late phase of gametogenesis. Their light-induced expression is restricted to cells that have completed the first, nitrogen starvation-activated, phase of differentiation. A comparison of the two genes revealed striking similarities as well as differences. Their most prominent shared feature is an extended sequence homology of over 90% in their 5′-untranslated regions, suggesting a role in translational regulation. GAS28 and GAS29 both encode hydroxyproline-rich proteins (HRGPs) of very similar sizes that exhibit typical features of volvocalean cell wall constituents. GAS28 shows a high degree of homology with the Volvox pherophorin gene family, suggesting a relationship between these genes.
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Cellular and molecular life sciences 10 (1954), S. 14-15 
    ISSN: 1420-9071
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Zusammenfassung Es wird gezeigt, dass die Hyperkonjugation einen entscheidenden Einfluss auf den Siedepunkt der einfach ungesättigten aliphatischen Kohlenwasserstoffe hat. Dieser Zusammenhang wird durch vergrösserte Elektronendelokalisierung in Molekülen, die Hyperkonjugation aufweisen, theoretisch verständlich. Ein grösseres Ausmass der Elektronendelokalisierung führt zu stärkerer Kohäsion und dadurch zu erhöhten Siedepunkten. Die neue Gesetzmässigkeit ergänzt ohne Widerspruch die bekannten Hückelschen Siedepunktsregeln und erklärt Beobachtungen, die aus keiner der bisher aufgestellten Regeln ableitbar waren. Es ist zu erwarten, dass analoge Zusammenhänge in anderen Gruppen von organischen Verbindungen aufgewiesen werden können.
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Physics and chemistry of minerals 27 (2000), S. 665-678 
    ISSN: 1432-2021
    Keywords: Key words Amber ; Rumantite ; Succinite ; Pyrolysis ; Gas chromatography ; Mass spectrometry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Geosciences , Physics
    Notes: Abstract  Romanian amber (rumanite) has been considered to be a separate species of fossil resin for more than a century. While earlier investigators held it to be very similar to succinite (Baltic amber), modern scholars have assigned it a distinctly different botanical origin. We have found that almost all of the constituents of the ether-soluble fractions of 13 specimens of authentic rumanite identified by gas chromatography–mass spectrometry have previously been reported in the soluble fraction of succinite, including succinic acid and its monoterpene esters. Additionally and significantly, the soluble fraction of rumanite contains a number defunctionalized compounds that do not preexist in succinite, but that are produced by pyrolysis of whole succinite or of its insoluble polymeric fraction. Simultaneous methylation pyrolysis–gas chromatography–mass spectrometry of the polymeric fraction of seven of the rumanite specimens yielded further copious amounts of dimethyl succinate, a number of diterpene resin acid methyl esters, and additional defunctionalized compounds known to be pyrolysis products of succinite. The evidence shows conclusively that the botanical origin of rumanite is not distinct from that of succinite. Rather, rumanite is a succinite that has suffered partial thermal degradation in the course of the folding of the Oligocene Kliwa sandstone formation in which it is most commonly found.
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    The European physical journal 338 (1991), S. 61-65 
    ISSN: 1434-601X
    Keywords: 25.70.Cd ; 25.70.Ef
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract Excitation functions for12C+12C elastic and inelastic scattering to 2+ level have been measured over the energy range 30–60 MeV (cm) by 250 keV steps using the kinematical coincidence method. The intermediate structure resonances disappear aboveE cm=35 MeV while the broad and irregular structure becomes a general feature of the interaction at higher energies.
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  • 8
    ISSN: 1434-601X
    Keywords: 25.70.Cd ; 25.70.Ef
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract The 16 O+ 15 N reaction products have been studied by the γ-ray detection method in the CM energy range 15.5 to 36.1 MeV and by the kinematical coincidence method at energies ranging from E CM =20.6 to 33.5MeV. The γ-ray yield excitation function of the 16O 3− inelastic channel shows the existence of resonant structures. Two structures with ∼ 1.6 MeV width are observed in the large angle elastic scattering excitation function, they are correlated with the resonances seen in the inelastic channel. Angular momentum assignments were made from the elastic backward angular distributions.
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  • 9
    ISSN: 1434-601X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract The properties of the two-body channels populated in the35Cl +12C reaction, at a bombarding energy of 180 KeV, have been studied by means of a kinematical coincidence technique. Binary reaction products show full energy equilibration and isotropic angular distributions in the center-of-mass frame. The asymmetrical elemental distribution of the fully-damped yields, similar to that observed previously in the32S +24Mg reaction, suggests the occurence of fusion-fission rather than orbiting.
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  • 10
    ISSN: 1434-601X
    Keywords: 25.70.Jj ; 25.70.Lm ; 25.85.Ge
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract The binary decay properties of the47V nucleus, produced in the35Cl +12C reaction, have been investigated at the35Cl bombarding energiesE lab = 180 and 200 MeV by means of a kinematical coincidence technique. Binary reaction products show full energy equilibration and a characteristic 1/sin(θ cm) angular distribution. The elemental distribution of the fully-damped products is asymmetric, similar to what has previously been observed in the decay of the56Ni nucleus. Comparison with theoretical model predictions suggests the occurrence of a fusion-fission rather than orbiting process. Moreover the calculations performed using the Extended Hauser-Feshbach Method reproduce well the experimental fission yields. A general discussion of orbiting and fusionfission experimental data of light heavy-ion systems is presented in the framework of the calculated number of available open channels for these systems.
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