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  • 1
    Electronic Resource
    Electronic Resource
    Hans Grüneberg May 26, 1907–October 23, 1982 (1983)
    Springer
    Immunogenetics 18 (1983), S. 309-310 
    Details
    ISSN: 1432-1211
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00372463
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  • 2
    Electronic Resource
    Electronic Resource
    Probing discrete auroral arcs by ionospheric tomography (1998)
    Springer
    Annales geophysicae 16 (1998), S. 574-582 
    Details
    ISSN: 0992-7689
    Keywords: Ionosphere (Auroral ionosphere) ; Magnetospheric physics (Auroral phenomena) ; Radio science (Ionospheric physics)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences , Physics
    Notes: Abstract Optical observations of 557.7 nm and 630.0 nm emissions from discrete auroral arcs in the post-noon sector have been related to localised field-aligned enhancements in the spatial distribution of E- and F-layer electron density respectively seen in images reconstructed by ionospheric tomography. Results from two case studies are presented in which meridian scanning photometer and all-sky camera observations on Svalbard have been compared to electron-density structures found by tomographic inversion of measurements made by reception of radio signals at a chain of four stations at high latitude. The F-layer features are long-lived and show exact correspondence to the red-line emissions. Transient arcs in green-line intensity result in E-region structures that are resolved in one case, but not in another where the dynamic auroral forms are separated by less than one degree of latitude. The signature of an inverted-V precipitation event is clearly evident in one example.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s00585-998-0574-z
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  • 3
    Electronic Resource
    Electronic Resource
    Ionospheric signatures of magnetospheric boundaries in the post-noon sector (1999)
    Springer
    Annales geophysicae 18 (1999), S. 74-80 
    Details
    ISSN: 0992-7689
    Keywords: Ionosphere (auroral ionosphere; ionosphere-magnetosphere interactions; polar ionosphere)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences , Physics
    Notes: Abstract Spatial structures in ionospheric electron density revealed in a tomographic image have been identified with auroral forms and related to their sources in precipitating particles observed by DMSP satellites. The observations of plasma enhancements relate to discrete auroral arcs seen in the post-noon sector, identified by both red- and green-line emissions measured by a meridional scanning photometer. The features lie within a very narrow latitudinal band on L-shells where the satellite detectors observed electron precipitation classified as from the boundary plasma sheet (BPS). The harder particles are identified with an E-region structure, while further north the precipitation is softer, resulting in a localised F-layer blob and 630.0 nm emissions. A steep gradient in plasma density represent a signature in the ionosphere of the central plasma sheet (CPS)/BPS boundary. A transition to a less-structured F-layer is found on crossing the convection reversal boundary.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s00585-000-0074-2
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  • 4
    Electronic Resource
    Electronic Resource
    Compositional and structural control in bone regenerative coatings (1999)
    Springer
    Journal of materials science 10 (1999), S. 219-222 
    Details
    ISSN: 1573-4838
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine , Technology
    Notes: Abstract The development of a low-temperature method of producing bioactive coatings for medical implants has been shown to bypass the problems associated with high temperature processing routes, in particular the appearance of amorphous phases and non-stoichiometric hydroxyapatite (HA), and delamination of the coating from the substrate. An electric field/aqueous solution technique for producing adherent, crack-free calcium phosphate coatings on titanium and stainless steel substrates is described. The characteristics of the coating are a function of electrode spacing, supersaturation, temperature and current and voltage conditions. Scanning electron microscopy (SEM) characterized the surface morphology of the coatings, which were shown to be HA. The possibility of producing a coating of carbonate-substituted HA having the same chemical composition as bone apatite, and forming at physiological temperatures, has also been demonstrated. The size of the microstructure decreased and the morphology changed as the carbonate ion concentration in the calcium and phosphate ion solution increased. © 1999 Kluwer Academic Publishers
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1008906128187
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  • 5
    Electronic Resource
    Electronic Resource
    Rent-seeking with multiple winners (1993)
    Springer
    Public choice 77 (1993), S. 437-443 
    Details
    ISSN: 1573-7101
    Source: Springer Online Journal Archives 1860-2000
    Topics: Economics
    Notes: Abstract This paper examines the impact of the number of winners allowed by regulators on rentseeking expenditures. It is demonstrated in a widely used model that an increase in the number of winners will decrease total rent-seeking expenditures. This result is generally obtained regardless of whether the firms are risk-averse or risk-lovers. When regulators award coveted market franchises, there will be smaller welfare losses if more winners are allowed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01047881
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  • 6
    Electronic Resource
    Electronic Resource
    Plant regeneration from leaf protoplasts of Lycopersicon hirsutum f. hirsutum (1991)
    Springer
    Plant cell reports 9 (1991), S. 680-683 
    Details
    ISSN: 1432-203X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A protocol is described for high frequency plant regeneration from isolated leaf protoplasts of several genotypes of the wild tomato species Lycopersicon hirsutum f. hirsutum based on modified tomato protoplast culture methods. Three to four week old seedlings exposed to a low light pretreatment yielded protoplasts capable of sustained divisions on modified Lycopersicon Culture Medium. Plating efficiencies varied from 7.2%–25.9%. Colonies were transferred to modified solid greening medium after 25–35 days. Developing calli that turned bright green and produced dark green bud primordia were transferred to shoot induction medium. Shoot formation efficiencies ranged from 60%–85%. Shoots rooted easily and regenerated plants grown to complete maturity showed only transient somaclonal variation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00235356
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  • 7
    Electronic Resource
    Electronic Resource
    Amino-acid transport into cultured tobacco cells (1981)
    Springer
    Planta 153 (1981), S. 511-518 
    Details
    ISSN: 1432-2048
    Keywords: Amino-acid transport ; Arginine ; Cell culture (tobacco) ; Nicotiana ; Transport (arginine)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Arginine transport in suspension-cultured cells of Nicotiana tabacum L. cv. Wisconsin-38 was investigated. Cells that were preincubated in the presence of Ca2+ for 6 h prior to transport exhibited stimulated transport rates. After the preincubation treatment, initial rates of uptake were constant for at least 45 min. Arginine accumulated in the cells against a concentration gradient; this accumulation was not the result of exchange diffusion. Arginine uptake over a concentration range of 2.5 μM to 1 mM was characterized by simple Michaelis-Menten kinetics with a Km of 0.1 mM and a Vmax of 9,000 nmol g-1 fresh weight h-1. Transport was inhibited by several compounds including carbonylcyanide-m-chlorophenylhydrazone, 2,4-dinitrophenol, N,N′-dicyclohexylcarbodiimide, and N-ethylmaleimide. Inhibition by these compounds was not the result of increased efflux resulting from membrane damage. A variety of amino acids and analogs, with the exception of D-arginine, inhibited transport, indicating that arginine transport was mediated by a general L-aminoacid permease. Competition experiments indicated that arginine and lysine exhibited cross-competition for transport, with Ki values similar to respective Km values. Arginine transport and low-affinity lysine transport are probably mediated by the same system in these cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00385534
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  • 8
    Electronic Resource
    Electronic Resource
    Molecular marker analysis of Helianthus annuus L. 2. Construction of an RFLP linkage map for cultivated sunflower (1995)
    Springer
    Theoretical and applied genetics 91 (1995), S. 195-199 
    Details
    ISSN: 1432-2242
    Keywords: Restriction fragment length polymorphism (RFLP) ; Linkage map ; Helianthus annuus ; Sunflower cDNAs
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A detailed linkage map of Helianthus annuus was constructed based on segregation at 234 RFLP loci, detected by 213 probes, in an F2 population of 289 individuals (derived from a cross between the inbred lines HA89 and ZENB8). The genetic markers covered 1380 centiMorgans (cM) of the sunflower genome and were aranged in 17 linkage groups, corresponding to the haploid number of chromosomes in this species. One locus was found to be unlinked. Although the average interval size was 5.9 cM, there were a number of regions larger than 20 cM that were devoid of markers. Genotypic classes at 23 loci deviated significantly from the expected ratios (1∶2∶1 or 3∶1), all showing a reduction in the ZENB8 homozygous class. The majority of these loci were found to map to four regions on linkage groups G, L and P.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00220877
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  • 9
    Electronic Resource
    Electronic Resource
    Molecular marker analysis of Helianthus annuus L. 1. Restriction fragment length polymorphism between inbred lines of cultivated sunflower (1994)
    Springer
    Theoretical and applied genetics 89 (1994), S. 435-441 
    Details
    ISSN: 1432-2242
    Keywords: Restriction fragment length polymorphism (RFLP) ; Helianthus annuus ; Cytoplasmic male sterility (CMS) ; Restorer ; Maintainer
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract cDNA and PstI genomic clones have been used to assess levels of restriction fragment length polymorphism (RFLP) in Helianthus annuus and to determine the inter-relationships between a diverse set of 24 inbred lines. Of the cDNA clones screened 45% were useful as RFLP probes, compared to less than 20% from the PstI library, which showed high levels of redundancy for high copy sequences. Fifty-seven low-copy DNA probes (23 PstI and 34 cDNA clones) were used to fingerprint 12 maintainer (B) lines and 12 restorer (R) lines. The average number of RFLP variants per probe was found to be 3.2, with a mean polymorphic index of 0.49, indicating that high levels of nuclear DNA polymorphism are to be found in cultivated sunflower. Cluster and principal coordinate analysis of the fingerprinting data clearly separated the maintainer and restorer lines, but there was a degree of association between 2 unbranched R-lines and the B-line germ plasm pool.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00225378
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  • 10
    Electronic Resource
    Electronic Resource
    Somatic hybridization of sexually incompatible petunias: Petunia parodii, Petunia parviflora (1980)
    Springer
    Theoretical and applied genetics 57 (1980), S. 1-4 
    Details
    ISSN: 1432-2242
    Keywords: Petunia parodii ; Petunia parviflora ; Sexual Incompatibility ; Somatic hybridisation ; Horticulture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Somatic hybrid plants were regenerated following the fusion of leaf mesophyll protoplasts of P. parodii with those isolated from a nuclear-albino mutant of P. parviflora. Attempts at sexual hybridization of these two species repeatedly failed thus confirming their previously established cross-incompatibility. Selection of somatic hybrid plants was possible since protoplasts of P. parodii would not develop beyond the cell colony stage, whilst those of the somatic hybrid and albino P. parviflora produced calluses. Green somatic hybrid calluses were visible against a background of albino cells/calluses, and upon transfer to regeneration media gave rise to shoots. Shoots and the resultant flowering plants were confirmed as somatic hybrids based on their growth habit, floral pigmentation and morphology, leaf hair structure, chromosome number and Fraction 1 protein profiles. The relevance of such hybrid material for the development of new, and extensively modified cultivars, is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00276001
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