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  • 1
    Electronic Resource
    Electronic Resource
    Hemopoietic functions of marrow-derived osteogenic cells (1992)
    Springer
    Calcified tissue international 51 (1992), S. 195-201 
    Details
    ISSN: 1432-0827
    Keywords: Marrow stroma ; Stromal cell lines ; Osteoblasts ; Cytokines ; MBA-15
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Summary Osteoblasts, members of the marrow stromal cellular network, may play an active role in the hemopoietic microenvironment as well as in bone remodeling. In this study, we examined the extent to which marrow-derived osteogenic cells (MBA-15) possess various stromal functions. This marrow stromal-derived cell line was shown by us to exhibit osteoblastic characteristics in culture and to form bone in vivo. These cells are shown here to constitutively produce and secrete cytokines identified as M-CSF, GM-CSF, and IL-6. MBA-15 cells modulate growth of normal and malignant myeloid and lymphoid cells as well as leukemia cell lines in vitro. Cell-cell interactions were studied in co-cultures with adherent MBA-15 cells and the target hemopoietic cells. Growth inhibition effects, observed under various experimental conditions, can be attributed to the presence of different soluble and membrane-bound inhibitory activities produced by MBA-15 cells. Thus, MBA-15 cells spontaneously produce both stimulators and inhibitors that can affect myeloid and lymphoid cell growth. Marrow osteogenic cells may therefore participate in the stromal regulation of hemopoiesis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00334547
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  • 2
    Electronic Resource
    Electronic Resource
    Histomorphometric Analysis of Heterotopic Bone Formed by Stromal-Osteogenic Subpopulations (1996)
    Springer
    Calcified tissue international 59 (1996), S. 254-258 
    Details
    ISSN: 1432-0827
    Keywords: Key words: Ectopic bone formation — Marrow osteoblasts — Cell differentiation — Histomorphometry.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Abstract. MBA-15.4 and MBA-15.6 cell lines are marrow stromal clonal subpopulations and represent various stages of differentiation of the osteoblastic family. These cells vary in terms of morphology, proliferation rate, synthesis of matrix proteins as collagen and noncollagenous proteins, and by their responses to hormones and growth factors. Their differential properties directly reflect the clonal cells' ability to form bone in vivo. When the cells were transplanted at an ectopic site, under the kidney capsule, MBA-15.4 line formed small foci of bone whereas MBA-15.6 cell line formed massive woven bone during the same period of time. In this study, we focused on the histomorphometric analysis of ectopic ossicles formed by the clonal cell lines. Assessments of bone mass changes involved measurements of cellular components, osteoid, and formation of primary bone. The bony tissue formed was condensed, no hemopoiesis was noted, and the ossicle was not remodeled. The histology studies were used for quantitative analysis of the ossicle formation and describe the dynamics of ossicles formed by the individual cell types.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002239900119
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  • 3
    Electronic Resource
    Electronic Resource
    Mineralization of marrow-stromal osteoblasts MBA-15 on three-dimensional carriers (1994)
    Springer
    Calcified tissue international 55 (1994), S. 120-127 
    Details
    ISSN: 1432-0827
    Keywords: Mineralization ; In vitro ; Stromal osteoblasts ; 3-D culture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Abstract The present study describes a new three-dimensional (3-D) culture system that enables the maintenance and phenotypic expression of bone marrow stromal osteoblasts. This culture substratum is advantageous in that it provides suitable conditions for attachment, growth, and differentiation of cells forming 3-D layers. The MBA-15 cell line was grown in unlimited quantities on 3-D Fibro-Cel carriers. These cells mineralized when exposed to ascorbic acid and β-glycerophosphate (βGP). Under these mineralization conditions, mRNA expressions of procollagen α2(I) and [3H]-proline-labeled protein were increased. The expression of mRNA for osteonectin and to a lesser extent, for osteopontin was increased, whereas alkaline phosphatase and biglycan remained unaffected under similar conditions. Exposure of mineralizing cultures to dexamethasone reduced mRNA of procollagen α2(I) and osteonectin to control level. Scanning electron microscopy revealed that cells were grown along the fabric's fibers and produced collagen fiberils. Under appropriate conditions, extensive mineralization had taken place. The mineralization process involves the formation of calcospherites, and correlates with an increase in calcium content. The Fibro-Cel carriers enable formation of 3-D architecture and mineralized tissue in vitro.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00297187
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