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RFLP Mapping of QTL for Fusarium Head Blight Resistance in Wheat (1999)

Waldron, B. L. ; Moreno-Sevilla, B. ; Anderson, J. A. ; [et al.]

Springer

Crop science 39 (1999), S. 805-811

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ISSN: 1435-0653

Source: Springer Online Journal Archives 1860-2000

Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition

Notes: Fusarium graminearum Schwabe (telemorph:Gibberella zeae) , in the USA and Canada have caused severe yield and quality losses in wheat (Triticum aestivum L.). Development of resistant cultivars has been difficult because of the complex inheritance of resistance and confounding environmental effects. This study was conducted to identify and map DNA markers linked to genes associated with FHB resistance. A population of 112 F5-derived recombinant inbred (RI) wheat lines from the cross 'Sumai 3' (resistant)/'Stoa' (moderately susceptible) was evaluated in two greenhouse experiments for Type II resistance (spread within the spike). On the basis of restriction fragment length polymorphism (RFLP) marker analyses, five genomic regions were significantly (P 〈 0.01) associated with FHB resistance, three derived from Sumai 3 and two from Stoa. Regions on Chromosomes 3BS (from Sumai 3) and 2AL (from Stoa) were identified by interval analysis using a LOD threshold of 3.0. These two quantitative trait loci (QTL) have been assigned the gene designations AFhs.ndsu-3B and QFhs.ndsu-2A, respectively. Recombinant inbred lines with these two QTL had a median severity of 20.9%, compared with 36.2% for all RI lines. The best RFLP marker in the 3BS region explained 15.4% of the variation and a multiple regression model consisting of three QTL explained 29.5% of the variation. These results indicate that resistance to FHB is inherited in a quantitative manner and that marker-assisted selection may aid the development of FHB-resistant cultivars.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/

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Computerised method for acquisition and display of gastrointestinal motility data (1991)

Waldron, B. ; Storey, B. E. ; Smith, D. ; [et al.]

Springer

Medical & biological engineering & computing 29 (1991), S. 304-308

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ISSN: 1741-0444

Keywords: Computer ; Motility ; Signal processing

Source: Springer Online Journal Archives 1860-2000

Topics: Biology , Chemistry and Pharmacology , Medicine

Notes: Abstract A computerised system is developed for the acquisition and display of gastrointestinal motility data which utilises a purpose developed software program called ‘PC-motil’, running on an IBM compatible microcomputer. ‘PC-motil’ displays data during collection, writes data to disk file and compresses all data at the end of a study on to a single monitor screen for convenient overview. Any area of interest, in single or multiple channels, may be selected and expanded for detailed examination. This system is tested by the recording of gastric and jejunal motility patterns of 11 healthy volunteers in fasting and fed states. All antral and jejunal migrating motor complexes (MMCs) in fasting studies, as well as all fed motility patterns were recognisable in both ‘compressed’ and ‘expanded’ form. The reproduction of motility patterns by the computer based system was indistinguishable from that of a conventional analogue chart recorder. This computerised system provides a convenient and cost-effective means of acquisition, storage and display of motility data in digital form.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF02446713

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