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  • Springer  (226)
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  • 1
    ISSN: 1432-072X
    Keywords: Key words K+-ATPase ; Lactobacilli ; Potassium pump ; 2 ; 3-Butanedione ; Enzyme-phosphate complex
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Lactobacillus helveticus ATCC 15009 (wild-type) membrane preparations hydrolyzed Mg2+-ATP as a function of K+ concentration (2–200 mM). Mg2+-ATP hydrolysis by L. helveticus membranes was strongly inhibited in the absence of exogenous K+, while it amounted to 6 nmol ATP hydrolyzed min–1 (mg membrane protein)–1 at 50 mM KCl (saturating conditions) and pH 7.2. The K+-dependent ATPase of L. helveticus displayed a relatively high affinity for potassium ions (K m = 800 μM) and was not affected by pretreatment of membranes with N,N’-dicyclohexylcarbodiimide. Membrane preparations were subjected to hypotonic shock to obtain a maximum yield of open profiles. The formation of a maximum level of enzyme-phosphate complex with a molecular mass of approximately 82 kDa was induced upon treatment of L. helveticus membrane preparations with low concentrations of [γ-32P]ATP in the presence of K+ and La3+ ions and was visualized by acidic SDS-PAGE. It was concluded that L. helveticus membranes contain an inwardly directed K+ pump whose presence is discussed in terms of its putative role in cytoplasmic pH regulation.
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  • 2
    Publication Date: 1997-09-08
    Print ISSN: 0302-8933
    Electronic ISSN: 1432-072X
    Topics: Biology
    Published by Springer
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Pure and applied geophysics 7 (1945), S. 1-15 
    ISSN: 1420-9136
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences , Physics
    Description / Table of Contents: Summary Applying the proceedings of Malkin verified by experiments of Ramsayer is studied the influence on the declination in upper levels of some magnetic anomalies in Italy, for which exist detailed reliefs in the vertical component (Colli Euganei, Stromboli). For the anomalous zones capable to influence the maintaining of the route in compass-airnavigation special maps are proposed containing the position of the isogons at various fondamental heights, an instance of which is presented for the isle of Stromboli. An examination of the magnetic anomalies in Italy shows the zones of probable and those of secure danger for compass-airnavigation as long as it will not be possible to prepare maps in the proposed type. For the rest of the Italian air-space the opportunity is prospected to construct the isogons for the pilots on the basis of the normal field.
    Abstract: Zusammenfassung Nach dem Malkinschen Verfahren wird der Einfluss einiger magnetischer Störgebiete (Colli Euganei, Stromboli) auf die Flugnavigation berechnet. Es wird vorgeschlagen, wo nötig, spezielle Deklinationskarten mit den Isogonen zu verschiedenen Haupthöhen vorzubereiten; ein Beispiel hiefür wird für die Insel Stromboli gegeben. Die Störgebiete, welche eine Gefahr für die Flugnavigation mit dem Magnetkompass bilden können, werden für Italien begrenzt. In dem übrigen Raum in Italien wird als das beste Verfahren für die Flugnavigation vorgeschlagen, die Isogonen nach dem vom Verf. berechneten Normalfeld darzustellen.
    Notes: Riassunto Con i procedimenti del Malkin, verificati sperimentalmente dal Ramsayer, viene intrapreso lo studio dell'influsso sulla declinazione in quota di alcune anomalie magnetiche in Italia, per cui esistono rilievi di dettaglio nella componente verticale (Colli Euganei, Stromboli). Per le zone anomale, capaci di influenzare il mantenimento della rotta nella navigazione aerea con la bussola, vengono proposte carte speciali con l'andamento delle isogone a varie quote fondamentali, di cui si presenta un saggio per l'isola di Stromboli. Attraverso un esame delle anomalie magnetiche in Italia, si indicano le zone di probabile e quelle di sicuro pericolo per la navigazione aerea con la bussola finchè non si potrà costruire per esse carte del tipo proposto. Per il rimanente spazio aereo italiano si prospetta l'opportunità che le isogone per i piloti siano costruite sulla base del campo normale.
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Pure and applied geophysics 9 (1946), S. 1-18 
    ISSN: 1420-9136
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences , Physics
    Description / Table of Contents: Summary Principally, on the base of the numerous measures of gravity established after 1930, and of the new compensated values for the reference stations, and with suitable hypothesis to reduce to a minimum the causes of error, which are examined and discussed, it is calculated the value ofg e in the two hypothesis ofBouguer and isostatical (h c =−113,7 km). The result is 978,043 in Potsdam System.
    Abstract: Zusammenfassung Auf Grund vor allem der zahlreichen nach 1930 ausgeführten Schweremessungen, der neuen kompensierten Werte fur die Hauptstationen, und durch geeignete Hypothesen um die Fehlerursachen, welche geprüft und erötert werden, zu reduzieren, wird esg e in den beiden Hypothesen vonBouguer und der Isostasie (h c =−113,7 km) berechnet. Das Resultat ist 978,043 im Potsdamer System.
    Notes: Riassunto Sulla base soprattutto delle numerose misure di gravità successive al 1930, dei nuovi valori compensati per le stazioni di riferimento e con opportune ipotesi per ridurre al minimo le cause di errore, che vengono esaminate e discusse, si procede al calcolo dig e nelle due ipotesi diBouguer ed isostatica (hc=−113,7 km). Il risultato è 978,043 nel sistema di Potsdam.
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  • 5
    Electronic Resource
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    Springer
    Pure and applied geophysics 18 (1950), S. 143-147 
    ISSN: 1420-9136
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences , Physics
    Description / Table of Contents: Summary It is shown how is possible to give pratical applications toEötvös' formulas on relations between gravitational and magnetic anomalies, in such a way as to obtain informations on magnetic properties of rocks that cause the anomaly. It follows the opportunity of executing together with torsion balance measures, in as much as possible, also magnetic measurements.
    Notes: Riassunto Viene mostrato come sia possibile dare pratiche applicazioni alle formule diEötvös sulle relazioni fra anomalie gravimetriche e magnetiche, in modo da ricavare le proprietà magnetiche delle rocce che causano l'anomalia. Da ciò l'opportunità di eseguire sempre unitamente a misurazioni con la bilancia di torsione anche misure magnetiche.
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  • 6
    Electronic Resource
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    Springer
    Pure and applied geophysics 8 (1946), S. 81-93 
    ISSN: 1420-9136
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences , Physics
    Description / Table of Contents: Summary It is shown that the «conventional» base-value of gravity at Potsdam (981, 274), considered until few years ago as one of the more precisious results, is instead affected very probably by a big error. This is demonstred first by the new absolute measure at Washington, and seems to be confirmed by the absolute measure at Leningrad too, for which definitive results are not yet known. — A new «international» reference system is therefore proposed, based no more on only one absolute measure of gravity, but on the most important ones until now executed. The relative differences between them result from a new compensation of the international net of the reference-stations excuted from the Author. — We shall be very thankfull to all those Geodesists who will kindly begun the discussion on this problem for its definitive solution.
    Abstract: Resumé Ce travail a le but d'exposer le plus clairement possible le problème du système de référence pour les mesures de gravité rélative et de montrer que la valeur fondamentale «conventionnelle» de Potsdam (981,274), considerée il y a quelques années comme un des résultats de la plus grande précision, se présent au contraire frappée très probablement d'une faute considérable. Tout cela est démontré surtout par la nouvelle mesure absolue faite à Washington et il semble être confirmé même par la mesure absolue de Leningrad, dont nous ne connaissons pas encore les résultats définitifs. — C'est pour cela que nous proposons l'adoption d'un nouveau système de référence international, qui soit fondé non plus sur le résultat d'une seule mesure absolue de la gravité, mais sur les plus importantes effectuées jusqu'ici. Les différences rélatives entr'elles resultent d'une nouvelle compensation du reseau international des stations de référence executée par l'Auteur. — Nous serons bien grès à tous ces Géodésiens qui voudront bien commencer la discussion sur ce problème, pur sa résolution définitive.
    Notes: Riassunto Il presente lavoro si prefigge lo scopo di esporre il problema del sistema di riferimento per le misure di gravità relativa e di mostrare che il valore base «convenzionale» di Potsdam (981.274), considerato fino a pochi anni addietro come uno dei risultati di maggiore precisione, si presenta invece affetto molto probabilmente da un notevole errore. Ciò viene dimostrato principalmente dalla nuova misura assoluta al Nat. Bureau of Standards di Washington, e sembra essere confermato anche dalla misura assoluta di Leaingrado, di cui non ci sono ancora noti i risultati definitive. — Si propone perciò l'adozione di un nuovo sistema di riferimento «internazionale», basato cioè non più sul risultato di una sola misura assoluta della gravità, ma sulle più importanti finora eseguite. Le differenze relative fra di esse risultano da una nuova compensazione della rete internazionale delle stazioni di riferimento eseguita dall'A. — Saremo molto grati a tutti quei Geodeti che vorranno cortesemente iniziare la discussione su questo problema.
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  • 7
    Electronic Resource
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    Springer
    Pure and applied geophysics 6 (1944), S. 14-22 
    ISSN: 1420-9136
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences , Physics
    Description / Table of Contents: Zusammenfassung Das erdmagnetische Feld in Albanien ist (zum ersten Mal) von Ital. Mil. Geogr. Institut studiert worden. Ein magnetisches Netz erster Ordnung von 65 Stationen (ungefähr 20 Km Punktabstand) inD, H undI wurde gemessen. Die Ergebnisse sind in einer Karte dargestellt, Massstab 1∶750.000, Isolinien am 1942,0. Ausser zwei ausgedehnten Störgebieten von Eruptivgesteinen (Eozän) in Nord-und Süd-Ost-Albanien, sind drei andere Regionalstörungen im Gebiet NO von Scutari, SW von Kruja und NW Koriza, und eine Lokalstörung SO von Valona zu erkennen. Die erste und die dritte sind wahrscheinlich den Ergussgesteinen zuzuschreiben.
    Notes: Riassunto Il campo magnetico terrestre in Albania è stato studiato (per la prima volta) dall'Istituto Geografico Militare attraverso l'esecuzione di una rete del 10 crdine (65 stazioni assolute nei tre elementiD, H edI a circa 20 Km l'una dall'altra). I risultati sono compendiati in una carta magnetica della regione, scala 1∶750.000, isolinee al 1942.0. Oltre a due zone molto estese di anomalie dovute a rocce eruttive del periodo eocenico nell' Albania settentrionale e sud-orientale, sono messe in evidenza altre tre importanti anomalie regionali nella regione a NE di Scutari, a SO di Kruja, a NE di Korcia ed una locale a SE di Valona. L'esame geofisico indica come probabile causa per la prima e la terza la presenza di rocce basiche a profondità non molto rilevanti (dell' ordine di qualche Km).
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  • 8
    Electronic Resource
    Electronic Resource
    Springer
    Pure and applied geophysics 6 (1944), S. 23-36 
    ISSN: 1420-9136
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences , Physics
    Description / Table of Contents: Zusammenfassung Der Verfasser behandelt den Beitrag des ital. Geogr. Mil. Instituts zur Erkenntniss des erdmagnetischen Feldes in Italien und anderen Gegenden. Einzelheiten werden über die magnetischen Karten von Italien gegeben und die Deklinationskarten für Libien, Albanien, Dalmatien, Mittelmeer, Schweiz und Ostmark, usw. erwähnt. Für Italien sind auch die Karten der magnetischen (ΔD und ΔH) und gravimetrischen (Faye und Bouguer) Isanomalen konstruiert worden.
    Notes: Riassunto Si riferisce sul contributo portato dall'Istituto Geografico Militare alla conoscenza del campo magnetico terrestre in Italia ed altre regioni (Libia, Albania, Etiopia). Si forniscono particolari sulle carte magnetiche d'Italia e sulle carte delle isogone per la Libia, Albania, Dalmazia, Mediterraneo, Austria, Svizzera costruite dall'I.G.M. Per lo studio della geologia profonda in Italia sono state preparate anche le carte delle anomalie magnetiche (declinazione e componente orizzontale) e gravimetriche (secondo Faye e secondo Bouguer).
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  • 9
    Electronic Resource
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    Springer
    Microbial ecology 35 (1998), S. 205-212 
    ISSN: 1432-184X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Intestinal microbiota comprise a complex ecosystem whose equilibrium is crucial for the health of animal species. For humans, data exist on the microbiota composition in adult subjects, but few studies have addressed the microbiota composition in infants. In particular, data on the presence and species distribution of members of the genus Lactobacillus in newborns (less than one week old) are lacking. In the present work, the feces of healthy newborns were sampled to determine the taxonomic composition of Lactobacillus in the intestinal microbiota in a group of 16 neonates. In total, 1640 colony-forming units (CFU) were isolated, of which 420 grouped in the Lactobacillus genus by means of primary phenotypic characterization. The 420 isolates were further grouped into 125 strains on the basis of identical plasmid profiles. Of these 125 strains, 21 turned out to be permanent, i.e., they were identified in the feces of the same subject on several consecutive days. Sugar fermentation, DNA/DNA hybridization, and S-layer protein determination enabled us to classify 52 of the 125 strains as follows: L. paracasei (40 strains), L. delbrueckii sp. (1 strain), and L. acidophilus (sensu stricto) (11 strains). Based on the same criteria, the remaining 73 strains were tentatively allotted to the Johnson subgroup B, although hybridization experiments with probes specific for L. gasseri and L. johnsonii species were not performed. The presence of new species among these 73 strains cannot be excluded. Surprisingly, the obligately heterofermentative lactobacilli, L. reuteri in particular, were entirely absent from the feces of healthy newborns.
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  • 10
    ISSN: 1432-1041
    Keywords: Budesonide ; liver ; man ; sulphotransferase ; testosterone ; drug metabolism
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Medicine
    Notes: Abstract Budesonide, a corticosteroid used in the treatment of asthma and allergic reactions, is almost entirely cleared by metabolism in man. We describe the sulphation of budesonide in human liver and lung and provide evidences that the sulphation of budesonide is catalysed by testosterone sulphotransferase. A rapid and reproducible radiometric assay for budesonide sulphotransferase is described. Liver specimens were obtained from 35 men and 65 women and lung specimens from 2 women and 17 men. The average hepatic budesonide sulphation rate was significantly higher in men (41.1 pmol·min−1·ml−1) than women (28.2 pmol·min−1·mg−1). In the lung, the mean budesonide sulphation rate was 5.0 pmol·min−1·mg−1. Testosterone strongly inhibited the hepatic sulphation of budesonide, whereas p-nitrophenol and dopamine were poor inhibitors; the IC50 was 7.0 uM (testosterone), 320 uM (p-nitrophenol) and 510 uM (dopamine). The hepatic rates of testosterone, p-nitrophenol and dopamine sulphation were measured in the same samples assayed for budesonide sulphotransferase. There was a correlation between the hepatic rates of budesonide and testosterone sulphation (P〈0.001; r=0.810). The activity of testosterone sulphotransferase was significantly greater in men than women (22.0 vs. 17.2 pmol·min−1·mg−1), wheres those of dopamine and p-nitrophenol sulphotransferase were not sex dependent. The hepatic activity of budesonide sulphotransferase parallels that of testosterone suggesting that sulphation is an important reaction in the metabolism of budesonide.
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