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  • Articles  (131)
  • Springer  (131)
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  • Articles  (131)
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  • 1
    ISSN: 1432-0827
    Keywords: Key words: PTH receptor — Osteocalcin — Alkaline phosphatase.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Abstract. Primary fetal rat calvarial cell cultures were examined for the expression of different osteoblastic parameters at the single cell level and in the whole population. The presence of the parathyroid hormone (PTH) receptor was studied by employing receptor autoradiography. After 3 days of culture, 10% of the cells expressed the PTH receptor. Immunolocalization of osteocalcin in 3-day-old cell cultures was found to be strongly correlated with the presence of the PTH receptor. Alkaline phosphatase (APase) localization in 3-day-old cultures correlated with only 69% of the PTH receptor expressing cells. Our results show that in 3-day-old rat calvarial cell cultures, only about 10% of the cells show markers of osteoblastic differentiation. The presence of the PTH receptor is strongly correlated with the presence of osteocalcin, but less with the presence of APase, indicating that it is the mature osteoblast that expresses the PTH receptor. After 7 days of culture, most receptor labeling, APase, and osteocalcin expression was found in multilayered areas of cells (nodules).
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  • 2
    ISSN: 1432-1114
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract  This paper presents the experimental investigation of the application of an image restoration technique aimed at improving the accuracy of infrared surface temperature measurements of cubes placed in a vertical channel flow. These cubes, used to determine distributions of the local heat transfer coefficient, show large spatial temperature gradients. The image restoration technique, developed to correct the spatial image degradation, is based on the two-dimensional Optical Transfer Function constructed from line spread functions (LSF). The benefit of the application of the infrared image restoration technique is verified with temperature measurements acquired from a second independent technique.
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  • 3
    ISSN: 1432-0703
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering , Medicine
    Notes: Abstract. Data sets on CB concentrations in fish-eating mammals from five laboratories were combined to test and refine a pharmacokinetic model. Clear differences in PCB patterns were observed between species. The ability to metabolize chlorobiphenyl (CB) congeners with vicinal H-atoms only in the ortho- and meta-positions and with one ortho-chlorine substituent generally increased in the order otter 〈 cetaceans (harbor porpoise, common dolphin) 〈 phocid seals (harbor and grey seal), but the metabolism of congeners with vicinal H-atoms in the meta- and para-positions and with two ortho-chlorines increased in the order cetaceans 〈 seals 〈 otter. Both categories of congeners are probably metabolized by different families of cytochrome P450 (1A and 2B) of which levels apparently differed between the cetaceans, the pinnipeds, and the otter. Within-species CB patterns differed in a concentration-dependent manner. The induction of cytochrome P450 enzymes offers the most likely explanation for this phenomenon, but starvation could have a similar effect on occasion.
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  • 4
    ISSN: 1432-0630
    Keywords: PACS: 41.60.Cr; 42.60.By; 42.65.Ky
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Abstract.  The use of a Free-Electron Laser (FEL) allows the study of (non)linear optical properties of materials over unsurpassed large spectral intervals. As an example, we report on the use of a FEL as the infrared source in spectroscopic infrared–visible Sum-Frequency Generation (SFG). Employing the extremely wide tunability of the Free-Electron Laser for Infrared eXperiments (FELIX) at Rijnhuizen, we have studied the frequency dependence of the nonline ar susceptibility for sum- frequency generation in gallium phosphide between 20 and 32 μm in great detail. We have developed a short-pulse visible laser system that is highly synchronous with FELIX thereby creating a two-color setup that can be broadly applied. Resonantly enhanced SFG in alpha-quartz has been used to study the relative timing stability of FELIX and the synchronized picosecond-laser system.
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  • 5
    ISSN: 1432-0630
    Keywords: PACS: 07.65.Gj; 42.65.Ky; 78.66.-w
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: 2 , BaF2, MgO, LiNbO3 were determined. The nonlinear spectroscopy applications of SEW–FEL techniques to studies of a second-harmonic generation (SHG) on crystal surfaces in the regime of counterpropagating SEWs – the frequency dependence of efficiency and the influence of a thin film deposition on a quartz surface – are described.
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  • 6
    ISSN: 1432-0649
    Keywords: PACS: 79.20.D; 87.50.H; 41.60.C
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: 2 . Dynamic parameters such as the extension of the ablation cloud, the initial velocity and momentum of the ablated particles as well as the ablation threshold, the ablated mass, and the particle size were investigated. The ablation plume was made visible with a stroboscopic technique. For a fluence of 3.1 J/cm2 the average initial velocity of the ejected particles was deduced from the extension of the plume to range from 120–400 m/s. Measurements of the recoil momentum using a sensitive pendulum led to values between 0.5 and 2.0 mm g/s. All measured properties were related to the spectroscopically determined absorption coefficient of cornea αcornea. Where absorption due to proteins is high (at λ=6.2 and 6.5 μm), ablated mass, velocity and recoil momentum behave according to αcornea. For the first time, variations of the ablation plume from pulse to pulse were observed. Those, as well as the particle size, not only depend on the absorption coefficient, but also on the predominant absorber.
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  • 7
    ISSN: 1432-0649
    Keywords: PACS: 42.62.Fi; 82.40.Py
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract. We report a series of Raman-Rayleigh-LIF measurements in two turbulent natural-gas jet diffusion flames produced by the Delft piloted jet diffusion flame burner. The main objective of the Raman-Rayleigh-LIF measurements was to obtain detailed information on the major species concentrations in the flames. The measurements provide simultaneous data on temperature, the concentrations of the major species and the radicals OH and NO and mixture fraction. The application of the Raman technique in the undiluted natural-gas flames proves to be very challenging because of the high fluorescence interference levels. The interference contributions to the recorded Raman signals are identified and subtracted using empirical correlations between the Raman signals and the signals on fluorescence interference monitor channels. The calibration and data reduction of the Raman-Rayleigh and LIF signals are discussed in detail. The resulting dataset compares excellently with data from previous experiments. Because the Raman-Rayleigh-LIF data provide quantitative concentrations and accordingly quantitative mixture fractions, they form a valuable and useful extension of the existing database for the Delft piloted jet diffusion flame burner.
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  • 8
    ISSN: 1573-0867
    Keywords: acidification ; ANE ; ANR ; calcium ammonium nitrate ; cattle slurry ; dilution ; grassland ; nitric acid ; nitrogen ; residual effect
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Acidification of cattle slurry with nitric acid to pH 4.5 was studied on experimental dairy farms in the Netherlands as a measure to reduce ammonia losses. This paper presents the results of two field experiments, carried out to assess the utilization of nitrogen (N) from nitric acid treated cattle slurry following surface application to grassland. Three aspects were studied: (i) the utilization of N from nitric acid treated cattle slurry following a single surface application to grassland; (ii) the effects of multiple applications of nitric acid treated cattle slurry on N utilization, residual inorganic N in the soil and sward quality; and (iii) the residual effects of nitric acid treated slurry on DM and N yield. In these experiments undiluted acidified slurry (UAS) and diluted acidified slurry (DAS) were compared with calcium ammonium nitrate (CAN). Apparent N recoveries (ANR) and apparent N efficiencies (ANE) were calculated on the basis of inorganic N in applied fertilizer and slurries. Following single applications of UAS and CAN on the sward on the clay soil, the average ANR values of UAS and CAN were 0.74 and 0.75 kg kg-1, respectively. The average ANE values of UAS and CAN were 30.3 and 29.5 kg DM per kg N, respectively. Single applications of DAS and CAN on the sandy soil resulted in average ANR values of 0.65 and 0.84 kg kg-1 and average ANE values of 24.1 and 29.2 kg DM per kg N for DAS and CAN, respectively. On average, the direct ANR values after four successive applications of CAN, UAS or DAS were 0.82, 0.82 and 0.74 kg kg-1, respectively and the direct ANE values were 28.4, 27.8 and 27.0 kg DM per kg N. On the sandy soil, the amount of residual inorganic N in the soil after the 4th cut increased with increasing rates of inorganic N application, with a slightly higher amount on plots treated with acidified slurry than on plots fertilized with CAN. On the clay soil, the amount of residual inorganic N was not affected by N application rate nor N source. On average, the residual ANR values, in the unfertilized 5th and 6th cuts, of CAN, UAS or DAS were 0.08, 0.10 and 0.09 kg kg-1, respectively and the residual ANE values were 2.7, 3.3 and 3.0 kg DM per kg N. It was concluded that nitric acid treated cattle slurry is an effective N fertilizer on grassland and that the residual effect of nitric acid treated cattle slurry is only marginally higher than that of CAN. Repeated applications of nitric acid treated cattle slurry did not affect sward composition.
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  • 9
    Electronic Resource
    Electronic Resource
    Springer
    GeoJournal 41 (1997), S. 359-367 
    ISSN: 1572-9893
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geography
    Notes: Abstract The relationship between the university and the city is expressed, amongst others, by the transfer of knowledge from the university to local actors. The university’s role as a local source of knowledge is studied by analyzing the outflow of the two main knowledge assets to the local area: graduates and research results. By comparing the results from universities in the Netherlands and the United Kingdom comparative evidence is discussed.
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  • 10
    Electronic Resource
    Electronic Resource
    Springer
    Flow, turbulence and combustion 62 (1999), S. 53-68 
    ISSN: 1573-1987
    Keywords: jet flame ; flame structures ; methane combustion
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract Laser Doppler Anemometry (LDA) and Planar Laser-Induced Fluorescence (PLIF) measurements have been performed in a turbulent nonpremixed jet flame. One of the features of this configuration is a central co-axial fuel jet surrounded by a turbulent annular air flow. The whole is placed within a low-speed coflowing air stream. This three-flow system with turbulent primary air differs from flow systems used for nonpremixed jet flames reported in the literature and is very useful for obtaining information on the mixing process between fuel and primary air. Next to the characterization of the velocity field, special attention has been paid to the conditional seeding of the central fuel jet and of the annular air flow. Together with visualizations of the OH radical, an important combustion intermediate which is formed during combustion, and the NO radical, which is seeded to the central jet flow, the resulting statistics reveal the properties of small- and large-scale structures in the flame.
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