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  • Articles  (12)
  • Springer  (12)
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  • Articles  (12)
  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Marine biology 45 (1978), S. 93-95 
    ISSN: 1432-1793
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Design and operation of a new sampler for zooplankton are described. The sampler facilitates reliable duplicate samples; it can be closed at a defined depth during vertical towing, and can be rapidly reloaded for repeated tows.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Archives of microbiology 111 (1977), S. 225-238 
    ISSN: 1432-072X
    Keywords: Cyanobacteria ; Phycobiliproteins ; Allophycocyanins ; Hybrid proteins ; Immunology ; Protein evolution ; Cyanidium caldurium
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Allophycocyanins were purified from diverse cyanobacteria and one rhodophytan alga (Cyanidium caldarium). The native proteins are trimeric molecules with the structure (αβ)3. Representative native allophycocyanins and their α and β subunits were characterized with respect to molecular weight, amino acid composition, isoelectric point, absorption and fluorescence spectra and immunological properties. All of the allophycocyanins studied were strikingly similar with respect to each of these properties. Renatured α and β subunits of allophycocyanin were distinct immunologically from each other, and both cross-reacted with the antiserum to the native protein. Trimeric allophycocyanin was readily reconstituted from the purified α and β subunits. Formation of hybrid allophycocyanins was demonstrated by direct isolation and characterization of the hybrid proteins and by immunological techniques. The results support the view that allophycocyanins are a highly conserved group of proteins.
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  • 3
    ISSN: 1432-1017
    Keywords: K+ channel ; Chromaffn granule ; Membrane reconstitution ; Planar bilayer
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Physics
    Notes: Abstract A K+ channel was incorporated into voltage-clamped planar lipid bilayers from bovine chromaffin granules and resealed granule membranes (“ghosts”). It was not incorporated from plasma membrane-rich fractions from the adrenal medulla. The channel had a conductance of ∼ 400 pS in symmetric 450 mM KCI, with the permeability sequence K+ 〉 Rb+ 〉 Cs+ 〉 Na+ 〉 Li+, and was insensitive to both Ca2+ and charybdotoxin. It exhibited complex gating kinetics, consistent with the presence of multiple open and closed states, and its gating was voltage-dependent. The channels appeared to incorporate into bilayers with the same orientation, and were blocked from one side (the side of vesicle addition) by 0.2-1 mM TEA'. The block was slightly voltage-dependent. Acidification of resealed granule membranes in response to external ATP (which activated the vacuolartype ATPase) was significantly reduced in the presence of 1 mM intralumenal TEACI (with 9 mM KCl), and parallel measurements with the potential-sensitive dye Oxonol V showed that such vesicles tended to develop higher internal-positive membrane potentials than control vesicles containing only 10 mM KCI. 1 mM TEA+ had no effect on proton-pumping activity when applied externally, and did not directly affect either the proton-pumping or ATP hydrolytic activity of the partially-purified ATPase. These results suggest that chromaffin granule membranes contain a TEA+-sensitive K+ channel which may have a role in regulating the vesicle membrane potential.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Molecular biology reports 2 (1975), S. 65-72 
    ISSN: 1573-4978
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Yeast tRNAPhe has been labelled with 125I under conditions which conserve the tertiary structure. Significant labelling was only found to occur on specific cytidines in single stranded regions, while other cytidines in single stranded regions and all those in the double stranded region underwent iodination to a very small extent. The pattern obtained from iodine labelling satisfies the conformation of a model recently proposed for this tRNA.
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  • 5
    ISSN: 1573-5044
    Keywords: antibiotics ; bactericides ; contamination ; fungicides ; in vitro ; orchids ; shoot tip ; seed germination ; tissue culture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Amphotericin B, benomyl, gentamycin, nystatin, quintozene penicillin G, sodium omadine, and vancomycin singly and in several combinations have no deleterious effects on the germination of orchid seeds, but inhibit the growth in vitro of shoot tip explants.
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  • 6
    ISSN: 1871-4528
    Keywords: minimum stomatal resistance ; soil water ; Solanum tuberosum L. ; photosynthetic photon flux density ; vapour pressure deficit
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Leaf stomatal resistance varies considerably in response to changes in environmental conditions. Understanding cultivar differences in the response of stomata to these conditions is important for effectively simulating water-use and growth. In this study the stomatal resistance of three field grown potato (Solanum tuberosum L.) cultivars Atlantic. Monona and Norchip was observed in response to photosynthetic photon flux density, leaf to air vapour pressure difference and root zone available soil water. Substantial variation in stomatal resistance was accounted for by functions of the environmental variables for both Monona and Norchip. The explained variation for Atlantic was considerably less. Light saturation occurred near 500 μmol m−2 s−1. Increasing stomatal resistance above 1000 μmol m−2s−1 was also observed but is likely an artifact of stomatal closure on days in which mid-day leaf water potentials dropped below critical levels. Although the leaf presumably insufficient to obtain substantial effects. The soil water status was partially linked to the average 1000 h to 1500 h stomatal resistance on clear, sunny days. Significant differences were observed among the cultivars in the response of stomata to changes in the available soil water.
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    Potato research 37 (1994), S. 393-402 
    ISSN: 1871-4528
    Keywords: measurement ; LAI ; Solanum tuberosum L
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Leaf area index (LAI) is widely used in many facets of potato (Solanum tuberosum L.) modelling but direct measurements have historically been difficult. This investigation tested the accuracy of a commercially available instrument (LI-COR LAI-2000) for measuring LAI non-destructively on a potato crop. Accurate estimates of LAI were difficult to obtain with small plots of≈1 m2. Results from larger field plots were extremely favourable and indicate that non-destructive measurements of LAI in situ can routinely be estimated within 5 to 10% of the destructively measured LAI. Six thinning tests performed on four potato cultivars produced average root mean square error measurements of LAI that ranged from 0.09 to 0.27.
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  • 8
    Electronic Resource
    Electronic Resource
    Springer
    Potato research 40 (1997), S. 251-266 
    ISSN: 1871-4528
    Keywords: LAI ; canopy expansion ; radiation use efficiency ; Solanum tuberosum L. ; soil water
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The growth and duration of crop leaf area determines the amount of solar radiation intercepted by the canopy and therefore influences the extent of photosynthesis, evaporation, transpiration and final dry matter yield. The objective of this study was to develop cultivar specific relationships to estimate the daily leaf area index (LAI) for the potato crop (Solanum tuberosum L.) that included the effects of available soil water. The model is divided into three LAI growth stages, the durations of which are partially related to potato heat units (PHU). The LAI in the first stage is estimated from a cultivar specific leaf area-based radiation use efficiency index with a soil water reduction factor. The second stage involves the maintenance of a constant LAI with the duration related to both PHU and a soil water index that can accelerate senescence. The final stage includes a decrease in the LAI from a maximum to zero in response to a cultivar specific PHU accumulation. Model simulations compared favourably with independent LAI measurements obtained with a LI-COR plant canopy analyzer over two seasons.
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  • 9
    Electronic Resource
    Electronic Resource
    Springer
    Potato research 40 (1997), S. 267-276 
    ISSN: 1871-4528
    Keywords: Solanum tuberosum L. ; transpiration estimates
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary A heat balance method for measuring sap flow in actively growing plants was tested on three potato (Solanum tuberosum L.) cultivars Monona, Norchip and Atlantic. The method is based on applying a known heat source to the stem of an intact plant: by measuring the axial and radial heat dissipation the magnitude of sap flow can be quantified. The technique shows promise as 24 h accumulated sap flow measurements were highly correlated (r2=0.935, n=36) with gravimetric measurements of transpiration and produced an average root mean square error (RMSE) of 20 g d−1. The accuracy of the method improved as daily accumulated flow increased. Under low daily flow rates overall RMSE increased, supporting the need for a heat storage term in the heat balance equation. Short term flow rates were less significantly correlated (r2=0.764, n=1572) and produced an average RMSE of 2.89 g h−1. Identification of these short term flow rates during high flow periods may be difficult. The presence of water potential gradients and the likelihood of re-hydration during high flow events may have also affected the short term performance of the method. At low flow rates, many different errors may be introduced.
    Type of Medium: Electronic Resource
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  • 10
    Publication Date: 1977-01-01
    Print ISSN: 0302-8933
    Electronic ISSN: 1432-072X
    Topics: Biology
    Published by Springer
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