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  • Springer  (15)
  • National Academy of Sciences  (1)
  • 1
    Digitale Medien
    Digitale Medien
    Surface structures of frozen-etched penicillium conidiospores (1966)
    Springer
    Naturwissenschaften 53 (1966), S. 708-708 
    Details
    ISSN: 1432-1904
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie , Allgemeine Naturwissenschaft
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00602737
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  • 2
    Digitale Medien
    Digitale Medien
    Germ pores ofChaetomium ascospores (1967)
    Springer
    Naturwissenschaften 54 (1967), S. 521-522 
    Details
    ISSN: 1432-1904
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie , Allgemeine Naturwissenschaft
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01129384
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  • 3
    Digitale Medien
    Digitale Medien
    Increased Susceptibility of Mg- and Mn-Deficient Pumpkin Seedlings to Fungal Attack (1977)
    Springer
    Naturwissenschaften 64 (1977), S. 437-438 
    Details
    ISSN: 1432-1904
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie , Allgemeine Naturwissenschaft
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00508704
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  • 4
    Digitale Medien
    Digitale Medien
    The induction of taxol production in the endophytic fungus—Periconia sp from Torreya grandifolia (1998)
    Springer
    Journal of industrial microbiology and biotechnology 20 (1998), S. 259-264 
    Details
    ISSN: 1476-5535
    Schlagwort(e): Keywords: taxol; attenuation; benzoic acid; Periconia sp; endophyte
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: A Periconia sp was isolated from Torreya grandifolia (a relative of yew that does not synthesize taxol) near Huangshan National Park in the People’s Republic of China. This fungus, not previously known as a tree endophyte, was isolated from the inner bark of a small lower limb. When freshly isolated from the tree and placed in a semi-synthetic medium, the fungus produced readily detectable quantities of the anticancer drug taxol. Other taxol-producing endophytes were also isolated from this source. The production of taxol by Periconia sp was demonstrated unequivocally via spectroscopic and immunological methods. However, successive transfers of the fungus in semi-synthetic medium resulted in gradual attenuation until low production occurred even though fungal growth was relatively unaffected. Several compounds, known previously as activators of microbial metabolism, including serinol, p-hydroxybenzoic acid, and a mixture of phenolic acids, were capable of fully or partially restoring taxol production to otherwise taxol-attenuated cultures. The compound with the most impressive ability to activate taxol production was benzoic acid at 0.01 mM. Benzoic acid was not a taxol precursor.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1038/sj.jim.2900521
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  • 5
    Digitale Medien
    Digitale Medien
    Ultrastructure of nuclei isolated from plant protoplasts (1977)
    Springer
    Protoplasma 93 (1977), S. 267-274 
    Details
    ISSN: 1615-6102
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary A simple method, involving selective Triton X-100 membrane solubilization, has been developed for the isolation of nuclei from barley and tobacco protoplasts which gives a high yield of essentially pure nuclei. The isolated nuclei resembled those in leaf cells and protoplasts when the isolated nuclei were fixed for short times (2 hours, Medium II), except that their chromatin appeared to be more highly condensed and barley nuclei also lacked the outer nuclear membrane. When longer times of fixation (12 hours, Medium I) were used, the isolated nuclei lacked the characteristic condensed chromatin appearance.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01275658
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  • 6
    Digitale Medien
    Digitale Medien
    Pseudoscopic illusions in stereo views of freeze-etch replicas (1978)
    Springer
    Protoplasma 93 (1978), S. 473-475 
    Details
    ISSN: 1615-6102
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary The use of stereo pairs of freeze-etch replicas significantly contributes to the interpretation of spatial relationships of structures within fungal spores. However, great care must be taken to assure proper orientation of prints before publication in order to prevent pseudoscopic illusions (an optical illusion which produces an apparent reversal of surface elevations and depressions). AsHarold C. Conklin [2] pointed out in his letter to Science, this phenomenon is an ever present concern for users of vertical imagery. The sensation of seeing depth in two dimensional photographs is an illusion. The goal is to create the proper illusion. In order to convey the desired information to the reader it is essential that surface photographs be oriented as if the illumination were coming mostly from the top of the page and the shadows were projected toward the bottom of the page. Examples of neglecting this simple rule may be found in publications ranging from news magazines [5] to prestigeous scientific journals [3, 4]. Articles occasionally contain photographs which are rotated 180 degrees from the proper orientation and thus convex surfaces are displayed as concave surfaces and vice versa. In stereo pairs of electron micrographs of freeze-etch replicas pseudoscopic illusions are also caused by transposing the right hand micrograph with the left hand micrograph. When viewed in the proper orientation the various organelle spatial relationships and membrane surfaces, and other structures, are clearly discernable and easily understood. However when the same micrographs are transposed from left to right, entirely erroneous concepts are conveyed. For example protoplasmic surfaces appear as exoplasmic surfaces [1]. An organelle whose image is actually present will vanish leaving behind only its imprint.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01276559
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  • 7
    Digitale Medien
    Digitale Medien
    Ultrastructure of dormant and germinated sporangiospores ofRhizopus arrhizus (1973)
    Springer
    Protoplasma 77 (1973), S. 15-33 
    Details
    ISSN: 1615-6102
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary Ultrastructural investigations of dormant and germinated sporangiospores ofRhizopus arrhizus were conducted by the use of thin sectioning and freeze-etching procedures. Dormant spores contained deep furrows and prominent ridges with swellings along the sides of the ridges. The furrows and ridges almost disappeared as spores swelled during germination. The plasma membranes contained protuberances or depressions (approximately 50 nm diam) depending upon the nature of the fracture. Mitochondria in dormant spores were spherical and contained few cristae compared to mitochondria of germinated spores which were larger, more diverse in shape and contained abundant cristae. Treatments of spores with 20–25% glycerol prior to freeze-etching or thin sectioning resulted in the production of artifacts between the cell wall and the plasma membrane. Vesicles were rarely observed at the apices of young germ tubes, but were present in abundance in growing hyphal tips. Surface views of young germ tubes revealed the presence of microfibrils.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01287290
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  • 8
    Digitale Medien
    Digitale Medien
    Fine structure ofPenicillium megasporum conidiospores (1967)
    Springer
    Protoplasma 64 (1967), S. 75-88 
    Details
    ISSN: 1615-6102
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary An investigation was conducted on the fine structure ofP. megasporum conidiospores using both chemical fixation and the freeze-etching techniques. The spore cell wall was shown to consist of several layers, one of which is chiefly fibrillar in nature. The surface of the spore appears to be covered by ordered arrays of “rodlets” which are embedded in or resting upon a thin layer of homogenous material. At higher magnification, these “rodlets” appear as linear arrays of particles approximately 50 Å in diameter. This layer may be of a cutaneous nature. In addition, the plasma membrane was shown to be covered by particles, some of which appear to migrate to the inner layer of the cell wall. Numerous invaginations of the plasma membrane were also seen.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01257383
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  • 9
    Digitale Medien
    Digitale Medien
    Fine structure of germinatingPenicillium megasporum conidia (1967)
    Springer
    Protoplasma 64 (1967), S. 439-451 
    Details
    ISSN: 1615-6102
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary Penicillium megasporum conidia have spore walls consisting of several layers. There is no visible change in the outer wall layers during spore germination, but the inner layers increases in thickness on only one side of the spore, resulting in a rupture of the outer wall layers and subsequently in germ tube formation. Invaginations in the plasma membrane disappear as the germ tube forms and emerges, and the nucleus migrates into the developing germ tube. Mitochondria gather at the base of the germ tube during its formation. During germination, the amount of lipid in the spore decreases and portions migrate into the germ tube. Membrane-bound, electron dense bodies are present in resting spores. These bodies decrease in size as germination proceeds, and the cytoplasm in the developing germ tube appears much more electron dense than the cytoplasm within the spore.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01666542
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  • 10
    Digitale Medien
    Digitale Medien
    Ultrastructure of primary sporidia of a wheat-bunt fungus,Tilletia caries, during ontogeny and mating (1979)
    Springer
    Protoplasma 99 (1979), S. 189-202 
    Details
    ISSN: 1615-6102
    Schlagwort(e): Mating ; Sporidia ; Tilletia ; Ultrastructure ; Wheat bunt
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary Primary sporidia ofTilletia caries (DC.) Tul. are borne on denticles at the tips of promycelia. The promycelia contain many small vacuoles and mitochondria and numerous lipid bodies. As the primary sporidia develop, the promycelial cytoplasm passes into the nascent cells. Septa develop between the bases of mature sporidia and the tips of the denticles. Sporidia that abscise from the denticles commonly have prominent birth scars at their bases. The sporidia have very thin walls, few vacuoles, attenuated mitochondria, and numerous lipid bodies. Conjugation pegs are generally produced by both members of a conjugating pair of sporidia and there are bud scars where they emerge from the sporidia. The sporidial walls are apparently hydrolyzed during emergence of the pegs. Vesicles are sometimes present at the tips of the conjugation pegs and, before fusion, electron-dense accumulations are sometimes observed between the tips of adjacent pegs. The approaching conjugation pegs are precisely aligned prior to fusion, suggesting polar communication. The walls of the conjugation pegs fuse and then are hydrolyzed. Fused sporidia are relatively homogeneous in content. The nucleus in a sporidum is often close to the conjugation tube and occasionally is partly within the fusion tube.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01275734
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