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  • 1
    Electronic Resource
    Electronic Resource
    Relationship between transcript production and virus resistance in transgenic tobacco expressing the potato leafroll virus coat protein gene (1993)
    Springer
    Plant cell reports 13 (1993), S. 54-58 
    Details
    ISSN: 1432-203X
    Keywords: Genetically engineered resistance ; segregation ratios ; potato leafroll luteovirus ; coat protein ; transcript RNA
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The coat protein (CP) gene of potato leafroll luteovims (PLRV) was inserted into tobacco (Nicotiana tabacum) using disarmed Agrobacterium tumefaciens (LBA4404) containing a binary expression vector. PLRV CP gene transcript was detected in transgenic plants but its abundance differed between transformed lines. CP was not detected in virus-free transgenic plants. The segregation of kanamycin resistance in S1 seedling progenies (obtained by selfing transformed plants) indicated that multiple (up to five) integration events involving vector T-DNA had occurred in most transformants. However, the amount of detectable CP transcript was not related to the neomycin phosphotransferase II gene copy number. Multiplication of PLRV in mature transgenic plants was diminished by up to 6-fold; the greatest diminution was in those transformed lines in which most CP gene transcript was detected. However, S1 progeny seedlings of transgenic plants were no more resistant to infection, following inoculation with viruliferous aphids, than seedlings of non-transformed control plants.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00232316
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  • 2
    Electronic Resource
    Electronic Resource
    Characterization of dominant hamster cell mutants resistant to oxygenated sterols (1982)
    Springer
    Somatic cell and molecular genetics 8 (1982), S. 557-574 
    Details
    ISSN: 1572-9931
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Stable mutants of Dede and CHO cells, resistant to suppression of cholesterogenesis by oxygenated sterols, have been isolated in a single step. Luria-Delbrück fluctuation analysis indicated a random occurrence of resistance at a rate of 1×10 −7 mutations/cell/generation. Cholesterol biosynthesis, 3-hydroxy-3-methylglutaryl coenzyme A reductase activity, and growth of the mutant cells were coordinately resistant to oxygenated sterols in the culture medium, and this resistance was expressed as a dominant traint in somatic cell hybrids of the wild-type and mutant cells. The dominant resistance was employed in the selection of various cell hybrids. There was complete additivity of reductase activities in mixed lysates of inhibited wild-type and uninhibited mutant cells, indicating that cytosolic (in)activation factors were not causative of this resistance. We suggest that oxygenated sterols are (co)repressors in suppression of the synthesis of the reductase and that the resistance mutant phenotypes result from altered regulatory loci.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01542851
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  • 3
    Electronic Resource
    Electronic Resource
    A common region of loss of heterozygosity in Wilms' tumor and embryonal rhabdomyosarcoma distal to the D11S988 locus on chromosome 11p15.5 (1996)
    Springer
    Human genetics 〈Berlin〉 97 (1996), S. 163-170 
    Details
    ISSN: 1432-1203
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The development of Wilms' tumor has been associated with two genetic loci on chromosome 11: WTI in 11p13 and WT2 in 11p15.5. Here, we have used loss of heterozygosity (LOH) in Wilms' tumors to narrow the WT2 locus distal to the D11S988 locus. A similar region was apparent for the clinically associated tumor, embryonal rhabdomyosarcoma. We have also demonstrated that a constitutional chromosome translocation breakpoint associated with Beckwith-Wiedemann syndrome and an acquired somatic chromosome translocation breakpoint in a rhabdoid tumor each occur in the same chromosomal interval as the smallest region of LOH in Wilms' tumors and embryonal rhabdomyosarcoma. Finally, we report the first Wilms' tumor without a cytogenetic deletion that shows targeted LOH for 11p15 and 11p13 while maintaining germline status for 11p14.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02265259
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  • 4
    Electronic Resource
    Electronic Resource
    A new highly polymorphic DNA restriction site marker in the 5′ region of the human tyrosine hydroxylase gene (TH) detecting loss of heterozygosity in human embryonal rhabdomyosarcoma (1994)
    Springer
    Human genetics 〈Berlin〉 93 (1994), S. 349-350 
    Details
    ISSN: 1432-1203
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract We have isolated a new marker (cos11-5TH) that detects an MspI restriction fragment length polymorphism in the 5′ region of the human tyrosine hydroxylase gene (TH) on chromosome band 11p15.5. This region of human chromosome 11 contains several important loci for disease phenotypes including Beckwith-Wiedemann syndrome (BWS), Wilms' tumor, and embryonal rhabdomyosarcoma. Thus, identification of new polymorphic markers in this region are important for future gene mapping and linkage analyses. To better define the region of 11p15.5 deleted in embryonal rhabdomyosarcoma, this new marker was used to investigate allelic losses in embryonal rhabdomyosarcoma tumors.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00212038
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  • 5
    Electronic Resource
    Electronic Resource
    Loss of heterozygosity in malignant gliomas involves at least three distinct regions on chromosome 10 (1993)
    Springer
    Human genetics 〈Berlin〉 92 (1993), S. 169-174 
    Details
    ISSN: 1432-1203
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract A panel of glial tumors consisting of 11 low grade gliomas, 9 anaplastic gliomas, and 29 glioblastomas were analyzed for loss of heterozygosity by examining at least one locus for each chromosome. The frequency of allele loss was highest among the glioblastomas, suggesting that genetic alterations accumulate during glial tumor development. The most common genetic alteration detected involved allele losses of chromosome 10 loci; these losses were observed in all glioblastomas and in three of the anaplastic gliomas. In order to delineate which chromosome 10 region or regions were deleted in association with glial tumor development, a deletion mapping analysis was performed, and this revealed the partial loss of chromosome 10 in eight glioblastomas and two of the anaplastic gliomas. Among these cases, three distinct regions of chromosome 10 were indicated as being targeted for deletion: one telomeric region on 10p and both telomeric and centromeric locations on 10q. These data suggest the existence of multiple chromosome 10 tumor suppressor gene loci whose inactivation is involved in the malignant progression of glioma.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00219686
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  • 6
    Electronic Resource
    Electronic Resource
    Ion movements during energy-linked mitochondrial structural changes (1978)
    Springer
    Journal of bioenergetics and biomembranes 10 (1978), S. 23-44 
    Details
    ISSN: 1573-6881
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Physics
    Notes: Abstract The structure of isolated rat liver mitochondria has been observed in the electron microscope following incubation of the mitochondria in vitro under a variety of conditions. The results show that ultrastructural changes are only associated with the energization and deenergization of isolated mitochondria if the composition of the incubation medium permits ion movements in or out of the matrix. The mechanism of energy coupling does not appear to depend on these major mitochondrial structural changes. The addition of low levels of valinomycin greatly increases the rate at which the matrix compartment swells and shrinks on energization and deenergization even at low K+ concentrations.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00743225
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  • 7
    Electronic Resource
    Electronic Resource
    Experimental acidification of Little Rock Lake, Wisconsin (1986)
    Springer
    Water, air & soil pollution 31 (1986), S. 115-121 
    Details
    ISSN: 1573-2932
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract The controlled acidification of a two-basin lake is described. The lake was divided by a vinyl curtain in 1984; acidification of one basin began in 1985. Target pH values of 5.5, 5.0 and 4.5 are planned for 2-yr increments. Biotic and chemical responses and internal alkalinity generation are being studied. Baseline studies, initial results at pH 5.5, and predictions of lake responses to acidification are described.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00630825
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  • 8
    Electronic Resource
    Electronic Resource
    Experimental acidification of Little Rock Lake, Wisconsin: The first four years of chemical and biological recovery (1995)
    Springer
    Water, air & soil pollution 85 (1995), S. 1713-1719 
    Details
    ISSN: 1573-2932
    Keywords: lake acidification ; recovery from acidification ; ecosystem experiments
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract Little Rock Lake was experimentally acidified in 1984–1990 during which sulfuric acid was added to one basin, decreasing pH from 6.1 to 5.6, 5.1 and 4.7. The lake has been allowed to recover without manipulation since autumn 1990. By the third year of recovery, ∼40% of the change necessary to return to pre-acidification values of pH, acid neutralizing capacity (ANC), sulfate (SO4 2−) and calcium (Ca2+) had occurred. During recovery years 1–2, ANC was closely predicted by models based on acidification phase observations, but recovery during years 3–4 was slower than predicted. A possible explanation for the slowed recovery is acidification of the upper 0–5 cm of sediment, which acts as a sink for the ANC generated via SO4 2− reduction, the primary recovery mechanism. Trends for Zooplankton did not follow pH recovery very closely. Species diminished by acidification (e.g. Keralella cochlearis, Daphnia dubia) have not recovered, but species that dominated the community at pH 4.7 (e.g. K. taurocephala, D. catawba) have not maintained high populations. The time required for the Zooplankton community to recover to pre-manipulation conditions is uncertain. Delays also have been observed for the mayfly species Caenis, which had disappeared at pH 4.7. In contrast, reproductive success of largemouth bass (Micropterus salmonides) mirrored that observed during acidification; egg hatch and survival of young-of-the-year to autumn recurred when pH exceeded response levels documented during acidification. Overall, recovery has not closely followed the pattern predicted by acidification responses.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00477227
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  • 9
    Electronic Resource
    Electronic Resource
    Detection of potato virus Y in potato tubers: a comparison of polymerase chain reaction and enzyme-linked immunosorbent assay (1993)
    Springer
    Potato research 36 (1993), S. 13-20 
    Details
    ISSN: 1871-4528
    Keywords: reverse transcription ; PCR ; immunoassay ; virus detection ; ELISA
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary A method was devised by which specific sequences of potato virus Y (PVY) RNA could be detected in total tuber RNA extracts by reverse transcription into cDNA and amplification by polymerase chain reaction (PCR). This method of PVY detection was tested and compared with the antibody-trapped antigen form of enzyme-linked immunosorbent assay (ATA-ELISA) using monoclonal antibodies. Both PCR and ATA-ELISA could detect PVY reliably in progeny tubers taken from growing plants of cv. Record, or tubers stored for 3 weeks after harvest. The ability to detect PVY decreased substantially after tubers had been stored for 20 weeks at 10°C. ATA-ELISA detected virus in only half the tubers from infected plants. However, PCR detected PVY very inefficiently in infected tubers after 20 weeks storage.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02359829
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  • 10
    Electronic Resource
    Electronic Resource
    Neurons and their synaptic organization in the visual cortex of the rat (1977)
    Springer
    Cell & tissue research 183 (1977), S. 499-517 
    Details
    ISSN: 1432-0878
    Keywords: Visual cortex (Rat) ; Neurons ; Synaptic Organization ; Electron microscopy of Golgi preparations
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Cells in the visual cortex (area 17) of adult rats were impregnated by the rapid Golgi method and characterized by light microscopy. Selected cells were then sectioned for electron microscopy and their cytological characteristics and the pattern of synapses on their cell bodies and dendrites were studied Twelve classical pyramidal cells from layers II–VI, two pyramid-like cells from layer VI, two inverted pyramidal cells from layers V and VI, ten spine-free non-pyramidal cells from layers II–VI and two spinous non-pyramidal cells from layer IV were examined. The cytoplasmic features of the identified cells, where these could be discerned, corresponded to those previously reported for the different cell types in conventionally prepared tissue. Pyramidal Cells received exclusively type 2 synaptic contacts on their cell bodies, type 1 contacts on their dendritic spines and a mixture of synaptic types (type II predominating) on their shafts, where synaptic density was relatively low. This pattern of synaptic contacts was consistent for all portions of the dendritic tree; inverted pyramidal cells and pyramid-like cells showed the same synaptic organization as classical pyramids. The axon collaterals of pyramidal cells established type I contacts with dendritic spines (or, rarely, shafts) of unknown origin. Non-Pyramidal Cells received both type 1 and type 2 contacts (the former predominating) on their cell bodies and dendrites. The spinous variety also received type I contacts on their dendritic spines. Axon terminal of spine-free non-pyramidal cells established type II synaptic contacts with dendritic shafts of unknown origin. The similarity in synaptic organization between the spine-free and spinous non-pyramidal cells examined in this study suggest that the latter correspond to the sparsely spinous stellate cells rather than to the spinous stellate cells of cat and monkey visual cortex.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00225663
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